+Open data
-Basic information
Entry | Database: PDB / ID: 1g3n | ||||||
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Title | STRUCTURE OF A P18(INK4C)-CDK6-K-CYCLIN TERNARY COMPLEX | ||||||
Components |
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Keywords | cell cycle / signaling protein / Cyclin-dependent kinase / INK4 inhibitor / Viral cyclin | ||||||
Function / homology | Function and homology information cyclin D2-CDK6 complex / cell dedifferentiation / Drug-mediated inhibition of CDK4/CDK6 activity / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / FBXO family protein binding / lateral ventricle development / negative regulation of myeloid cell differentiation / type B pancreatic cell development / negative regulation of monocyte differentiation ...cyclin D2-CDK6 complex / cell dedifferentiation / Drug-mediated inhibition of CDK4/CDK6 activity / Evasion of Oncogene Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / Evasion of Oxidative Stress Induced Senescence Due to Defective p16INK4A binding to CDK4 and CDK6 / FBXO family protein binding / lateral ventricle development / negative regulation of myeloid cell differentiation / type B pancreatic cell development / negative regulation of monocyte differentiation / astrocyte development / dentate gyrus development / negative regulation of phosphorylation / gliogenesis / cyclin-dependent protein serine/threonine kinase inhibitor activity / regulation of cell motility / Regulation of RUNX1 Expression and Activity / regulation of hematopoietic stem cell differentiation / positive regulation of cell-matrix adhesion / generation of neurons / oligodendrocyte differentiation / regulation of cyclin-dependent protein serine/threonine kinase activity / regulation of G1/S transition of mitotic cell cycle / Defective binding of RB1 mutants to E2F1,(E2F2, E2F3) / negative regulation of cellular senescence / negative regulation of cell differentiation / negative regulation of cell cycle / hematopoietic stem cell differentiation / cyclin-dependent protein kinase holoenzyme complex / negative regulation of osteoblast differentiation / cyclin-dependent kinase / cyclin-dependent protein serine/threonine kinase activity / negative regulation of stem cell proliferation / Notch signaling pathway / ruffle / regulation of G2/M transition of mitotic cell cycle / cyclin binding / response to organic substance / stem cell proliferation / G1/S transition of mitotic cell cycle / response to virus / regulation of erythrocyte differentiation / Oncogene Induced Senescence / negative regulation of cell growth / Cyclin D associated events in G1 / positive regulation of fibroblast proliferation / negative regulation of epithelial cell proliferation / T cell differentiation in thymus / Senescence-Associated Secretory Phenotype (SASP) / regulation of gene expression / Oxidative Stress Induced Senescence / regulation of cell cycle / cell cycle / cell division / negative regulation of cell population proliferation / protein phosphorylation / protein serine kinase activity / centrosome / positive regulation of gene expression / protein kinase binding / negative regulation of transcription by RNA polymerase II / signal transduction / nucleoplasm / ATP binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Human herpesvirus 8 | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Jeffrey, P.D. / Tong, L. / Pavletich, N.P. | ||||||
Citation | Journal: Genes Dev. / Year: 2000 Title: Structural basis of inhibition of CDK-cyclin complexes by INK4 inhibitors. Authors: Jeffrey, P.D. / Tong, L. / Pavletich, N.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1g3n.cif.gz | 265.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1g3n.ent.gz | 216.3 KB | Display | PDB format |
PDBx/mmJSON format | 1g3n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g3/1g3n ftp://data.pdbj.org/pub/pdb/validation_reports/g3/1g3n | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 36987.328 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CYCLIN-DEPENDENT KINASE 6 / Production host: unidentified baculovirus / Strain (production host): HI5 / References: UniProt: Q00534, EC: 2.7.1.37 #2: Protein | Mass: 18149.357 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: P18(INK4C) / Production host: Escherichia coli (E. coli) / References: UniProt: P42773 #3: Protein | Mass: 28094.281 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human herpesvirus 8 / Genus: Rhadinovirus / Gene: K-CYCLIN (VIRAL CYCLIND HOMOLOG) / Production host: Escherichia coli (E. coli) / References: UniProt: O40946, UniProt: Q98147*PLUS |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.51 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.6 Details: (NH4)2SO4, Dioxane, Tris-HCl, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Mar 20, 1999 / Details: Mirrors |
Radiation | Monochromator: Mirror, Yale Design / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→100 Å / Num. all: 40272 / Num. obs: 40272 / % possible obs: 95 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Biso Wilson estimate: 55 Å2 / Rmerge(I) obs: 0.057 / Net I/σ(I): 17.4 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.299 / % possible all: 91.9 |
Reflection | *PLUS Lowest resolution: 100 Å / Num. measured all: 131920 |
Reflection shell | *PLUS % possible obs: 91.9 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.9→15 Å / Cross valid method: THROUGHOUT / σ(F): 1 / Stereochemistry target values: CNS
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Refinement step | Cycle: LAST / Resolution: 2.9→15 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.9 Å / Lowest resolution: 15 Å / σ(F): 1 / % reflection Rfree: 5 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |