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Yorodumi- PDB-1fhl: CRYSTAL STRUCTURE OF BETA-1,4-GALACTANASE FROM ASPERGILLUS ACULEA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fhl | ||||||
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Title | CRYSTAL STRUCTURE OF BETA-1,4-GALACTANASE FROM ASPERGILLUS ACULEATUS AT 293K | ||||||
Components | BETA-1,4-GALACTANASE | ||||||
Keywords | HYDROLASE / B/A barrel / glycosyl hydrolase / family 53 / clan GH-A | ||||||
Function / homology | Function and homology information arabinogalactan endo-beta-1,4-galactanase / arabinogalactan endo-1,4-beta-galactosidase activity / glucosidase activity / pectin catabolic process Similarity search - Function | ||||||
Biological species | Aspergillus aculeatus (mold) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.3 Å | ||||||
Authors | Ryttersgaard, C. / Larsen, S. | ||||||
Citation | Journal: Biochemistry / Year: 2002 Title: Aspergillus aculeatus beta-1,4-Galactanase: Substrate Recognition and Relations to Other Glycoside Hydrolases in Clan GH-A Authors: Ryttersgaard, C. / Leggio, L.L. / Coutinho, P.M. / Henrissat, B. / Larsen, S. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1999 Title: Crystallization and Preliminary X-ray Studies of beta-1,4-galactanase from Aspergillus aculeatus Authors: Ryttersgaard, C. / Poulsen, J. / Christgau, S. / Sandal, T. / Dalboge, H. / Larsen, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fhl.cif.gz | 71.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fhl.ent.gz | 57.9 KB | Display | PDB format |
PDBx/mmJSON format | 1fhl.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fh/1fhl ftp://data.pdbj.org/pub/pdb/validation_reports/fh/1fhl | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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Details | The biological assembly is a monomer |
-Components
#1: Protein | Mass: 36775.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Aspergillus aculeatus (mold) References: UniProt: P48842, arabinogalactan endo-beta-1,4-galactanase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.81 % | ||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 400, calcium chloride, sodium hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: Ryttersgaard, C., (1999) Acta Crystallogr.,Sect.D, 55, 929. | ||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Mar 14, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→28.8 Å / Num. all: 15582 / Num. obs: 15582 / % possible obs: 97.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.6 % / Biso Wilson estimate: 30.82 Å2 / Rmerge(I) obs: 0.096 / Net I/σ(I): 7.1 |
Reflection shell | Resolution: 2.3→2.42 Å / Redundancy: 4.4 % / Rmerge(I) obs: 0.392 / Num. unique all: 2154 / % possible all: 95.6 |
Reflection | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 28.9 Å |
Reflection shell | *PLUS % possible obs: 95.6 % / Mean I/σ(I) obs: 61.4 |
-Processing
Software |
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Refinement | Resolution: 2.3→28.8 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.3→28.8 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 28.9 Å | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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