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Yorodumi- PDB-1e8j: SOLUTION STRUCTURE OF DESULFOVIBRIO GIGAS ZINC RUBREDOXIN, NMR, 2... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1e8j | ||||||
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Title | SOLUTION STRUCTURE OF DESULFOVIBRIO GIGAS ZINC RUBREDOXIN, NMR, 20 STRUCTURES | ||||||
Components | RUBREDOXIN | ||||||
Keywords | ELECTRON TRANSPORT / ZINC-SUBSTITUTION / THERMOSTABILITY | ||||||
Function / homology | Function and homology information | ||||||
Biological species | DESULFOVIBRIO GIGAS (bacteria) | ||||||
Method | SOLUTION NMR / TORSION ANGLE DYNAMICS WITH SIMULATED ANNEALING | ||||||
Authors | Lamosa, P. / Brennan, L. / Vis, H. / Turner, D.L. / Santos, H. | ||||||
Citation | Journal: Extremophiles / Year: 2001 Title: NMR structure of Desulfovibrio gigas rubredoxin: a model for studying protein stabilization by compatible solutes. Authors: Lamosa, P. / Brennan, L. / Vis, H. / Turner, D.L. / Santos, H. #1: Journal: Appl.Environ.Microbiol. / Year: 2000 Title: Thermostabilization of Proteins by Diglycerol Phosphate, a New Compatible Solute from the Hyperthermophile Archaeoglobus Fulgidus Authors: Lamosa, P. / Burke, A. / Peist, R. / Huber, R. / Liu, M.-Y. / Silva, G. / Rodrigues-Pousada, C. / Legall, J. / Maycock, C. / Santos, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1e8j.cif.gz | 290.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1e8j.ent.gz | 241.5 KB | Display | PDB format |
PDBx/mmJSON format | 1e8j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e8/1e8j ftp://data.pdbj.org/pub/pdb/validation_reports/e8/1e8j | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5682.304 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: IRON-SULFUR-PROTEIN, ZINC SUBSTITUTED / Source: (gene. exp.) DESULFOVIBRIO GIGAS (bacteria) / Cellular location: CYTOPLASM / Plasmid: PRPPL1 / Cellular location (production host): CYTOPLASM / Gene (production host): RDDG / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): JM109 / References: UniProt: P00270 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D-1H-NOESY 2D-1H-TOCSY 2D-1H-COSY |
-Sample preparation
Sample conditions | pH: 7.6 / Temperature: 308 K |
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-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: TORSION ANGLE DYNAMICS WITH SIMULATED ANNEALING / Software ordinal: 1 | |||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 500 / Conformers submitted total number: 20 |