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Open data
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Basic information
Entry | Database: PDB / ID: 1bk2 | ||||||
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Title | A-SPECTRIN SH3 DOMAIN D48G MUTANT | ||||||
![]() | A-SPECTRIN | ||||||
![]() | SH3-DOMAIN / ![]() | ||||||
Function / homology | ![]() actin filament capping / ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Martinez, J.C. / Pisabarro, M.T. / Serrano, L. | ||||||
![]() | ![]() Title: Obligatory steps in protein folding and the conformational diversity of the transition state. Authors: Martinez, J.C. / Pisabarro, M.T. / Serrano, L. #1: ![]() Title: Crystal Structure of a Src-Homology 3 (SH3) Domain Authors: Musacchio, A. / Noble, M. / Pauptit, R. / Wierenga, R. / Saraste, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 25.4 KB | Display | ![]() |
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PDB format | ![]() | 14.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1shgS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 6637.656 Da / Num. of mol.: 1 / Fragment: SH3-DOMAIN / Mutation: D48G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.34 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | pH: 6 / Details: pH 6.0 | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 1, 1998 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→15 Å / Num. obs: 47630 / % possible obs: 96.5 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.044 |
Reflection shell | Resolution: 2→2.1 Å / Rmerge(I) obs: 0.121 / % possible all: 95.5 |
Reflection | *PLUS Num. obs: 4758 / Num. measured all: 47630 |
Reflection shell | *PLUS % possible obs: 95.5 % |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1SHG Resolution: 2.01→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 35.29 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.01→8 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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