+Open data
-Basic information
Entry | Database: PDB / ID: 2f2w | ||||||
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Title | alpha-spectrin SH3 domain R21A mutant | ||||||
Components | Spectrin alpha chain, brain | ||||||
Keywords | SIGNALING PROTEIN / Src homology 3 domain SPECTRIN | ||||||
Function / homology | Function and homology information actin filament capping / costamere / cortical actin cytoskeleton / cell projection / actin filament binding / cell junction / actin cytoskeleton organization / calmodulin binding / calcium ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Camara-Artigas, A. / Conejero-Lara, F. / Casares, S. / Lopez-Mayorga, O. / Vega, C. | ||||||
Citation | Journal: Proteins / Year: 2007 Title: Cooperative propagation of local stability changes from low-stability and high-stability regions in a SH3 domain Authors: Casares, S. / Lopez-Mayorga, O. / Vega, M.C. / Camara-Artigas, A. / Conejero-Lara, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2f2w.cif.gz | 25.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2f2w.ent.gz | 15.4 KB | Display | PDB format |
PDBx/mmJSON format | 2f2w.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f2/2f2w ftp://data.pdbj.org/pub/pdb/validation_reports/f2/2f2w | HTTPS FTP |
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-Related structure data
Related structure data | 2cdtC 2f2vC 2f2xC 1shgS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 7143.128 Da / Num. of mol.: 1 / Fragment: Src-homology 3 domain / Mutation: R21A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Gene: SPTAN1, SPTA2 / Production host: Escherichia coli (E. coli) / References: UniProt: P07751 |
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#2: Chemical | ChemComp-SO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.08 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 3 Details: Ammonium sulphate 2M, pH 3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: BW7B / Wavelength: 0.8414 Å |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: May 30, 2004 |
Radiation | Monochromator: flat pre-mirror, a single crystal sideways reflecting curved Si(111) monochromator and a vertical focusing mirror Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8414 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→20 Å / Num. obs: 6723 / % possible obs: 89.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4 % / Biso Wilson estimate: 14.1 Å2 / Rmerge(I) obs: 0.096 / Rsym value: 0.084 / Net I/σ(I): 5.5 |
Reflection shell | Resolution: 1.7→1.79 Å / % possible all: 89.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1SHG Resolution: 1.7→19.37 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 657797.79 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 58.0708 Å2 / ksol: 0.403266 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.1 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.7→19.37 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.7→1.81 Å / Rfactor Rfree error: 0.026 / Total num. of bins used: 6
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Xplor file |
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