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Yorodumi- PDB-1bbs: X-RAY ANALYSES OF PEPTIDE INHIBITOR COMPLEXES DEFINE THE STRUCTUR... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1bbs | ||||||
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Title | X-RAY ANALYSES OF PEPTIDE INHIBITOR COMPLEXES DEFINE THE STRUCTURAL BASIS OF SPECIFICITY FOR HUMAN AND MOUSE RENINS | ||||||
Components | RENIN | ||||||
Keywords | ASPARTIC PROTEINASE | ||||||
Function / homology | Function and homology information renin / juxtaglomerular apparatus development / mesonephros development / response to cGMP / renin-angiotensin regulation of aldosterone production / drinking behavior / regulation of MAPK cascade / response to immobilization stress / angiotensin maturation / amyloid-beta metabolic process ...renin / juxtaglomerular apparatus development / mesonephros development / response to cGMP / renin-angiotensin regulation of aldosterone production / drinking behavior / regulation of MAPK cascade / response to immobilization stress / angiotensin maturation / amyloid-beta metabolic process / Metabolism of Angiotensinogen to Angiotensins / cell maturation / response to cAMP / insulin-like growth factor receptor binding / hormone-mediated signaling pathway / kidney development / regulation of blood pressure / male gonad development / cellular response to xenobiotic stimulus / apical part of cell / peptidase activity / response to lipopolysaccharide / aspartic-type endopeptidase activity / signaling receptor binding / proteolysis / extracellular space / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.8 Å | ||||||
Authors | Dhanaraj, V. / Blundell, T.L. | ||||||
Citation | Journal: Nature / Year: 1992 Title: X-ray analyses of peptide-inhibitor complexes define the structural basis of specificity for human and mouse renins. Authors: Dhanaraj, V. / Dealwis, C.G. / Frazao, C. / Badasso, M. / Sibanda, B.L. / Tickle, I.J. / Cooper, J.B. / Driessen, H.P. / Newman, M. / Aguilar, C. / Wood, S.P. / Blundell, T.L. / Hobart, P.M. ...Authors: Dhanaraj, V. / Dealwis, C.G. / Frazao, C. / Badasso, M. / Sibanda, B.L. / Tickle, I.J. / Cooper, J.B. / Driessen, H.P. / Newman, M. / Aguilar, C. / Wood, S.P. / Blundell, T.L. / Hobart, P.M. / Geoghegan, K.F. / Ammirati, M.J. / Danley, D.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bbs.cif.gz | 122.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bbs.ent.gz | 100.9 KB | Display | PDB format |
PDBx/mmJSON format | 1bbs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bb/1bbs ftp://data.pdbj.org/pub/pdb/validation_reports/bb/1bbs | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 23 / 2: CIS PROLINE - PRO 111 / 3: CIS PROLINE - PRO 294 / 4: CIS PROLINE - PRO 297 | ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.7821, -0.3605, -0.5084), Vector: Details | THIS ENTRY CONTAINS ONLY ONE OF THE TWO PROTEIN CHAINS IN CRYSTALLOGRAPHIC ASYMMETRIC UNIT. THE TRANSFORMATION PRESENTED ON *MTRIX* RECORDS BELOW MAY BE USED TO GENERATE THE RELATED PROTEIN CHAIN. | |
-Components
#1: Protein | Mass: 37267.008 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / References: UniProt: P00797, renin Sequence details | THE REVISED 'PEPSIN NUMBERING' SCHEME IS USED FOR NUMBERING THE RESIDUES. | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.28 Å3/Da / Density % sol: 62.44 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 59.8 % | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.8 Å / Num. obs: 22383 / % possible obs: 88.6 % / Rmerge(I) obs: 0.076 |
-Processing
Software | Name: RESTRAIN / Classification: refinement | ||||||||||||
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Refinement | Rfactor obs: 0.196 / Highest resolution: 2.8 Å | ||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.8 Å
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Refine LS restraints |
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Software | *PLUS Name: RESTRAIN / Classification: refinement | ||||||||||||
Refinement | *PLUS Lowest resolution: 8 Å / Num. reflection obs: 21297 / Rfactor obs: 0.196 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS |