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- EMDB-33146: Cryo-EM structures of human mitochondrial NAD(P)+-dependent malic... -
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Open data
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Basic information
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Title | Cryo-EM structures of human mitochondrial NAD(P)+-dependent malic enzyme in a ternary complex with NAD+ and allosteric inhibitor EA | |||||||||
![]() | ME2-EA Full Map | |||||||||
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![]() | Human mitochondrial NAD(P)+-dependent malic enzyme (ME2) / ![]() ![]() | |||||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Wang CH / Hsieh JT / Ho MC / Hung HC | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Suppression of the human malic enzyme 2 modifies energy metabolism and inhibits cellular respiration Authors: Hsieh JY / Chen KC / Wang CH / Liu GY / Ye JA / Chou YT / Lin YC / Lyu CJ / Chang RY / Liu YL / Li YH / Lee MR / Ho MC / Hung HC | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 204 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.1 KB 15.1 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13.9 KB | Display | ![]() |
Images | ![]() | 212.5 KB | ||
Others | ![]() ![]() | 200.7 MB 200.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7xdfMC ![]() 7xdeC ![]() 7xdgC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | ME2-EA Full Map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.82 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: ME2-EA Half Map 1
File | emd_33146_half_map_1.map | ||||||||||||
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Annotation | ME2-EA Half Map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: ME2-EA Half Map 2
File | emd_33146_half_map_2.map | ||||||||||||
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Annotation | ME2-EA Half Map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : human mitochondrial NAD(P)+-dependent malic enzyme in a ternary c...
Entire | Name: human mitochondrial NAD(P)+-dependent malic enzyme in a ternary complex with NAD+ and allosteric inhibitor EA |
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Components |
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-Supramolecule #1: human mitochondrial NAD(P)+-dependent malic enzyme in a ternary c...
Supramolecule | Name: human mitochondrial NAD(P)+-dependent malic enzyme in a ternary complex with NAD+ and allosteric inhibitor EA type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: NAD-dependent malic enzyme, mitochondrial
Macromolecule | Name: NAD-dependent malic enzyme, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO EC number: ![]() |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 65.521434 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MLSRLRVVST TCTLACRHLH IKEKGKPLML NPRTNKGMAF TLQERQMLGL QGLLPPKIET QDIQALRFHR NLKKMTSPLE KYIYIMGIQ ERNEKLFYRI LQDDIESLMP IVYTPTVGLA CSQYGHIFRR PKGLFISISD RGHVRSIVDN WPENHVKAVV V TDGERILG ...String: MLSRLRVVST TCTLACRHLH IKEKGKPLML NPRTNKGMAF TLQERQMLGL QGLLPPKIET QDIQALRFHR NLKKMTSPLE KYIYIMGIQ ERNEKLFYRI LQDDIESLMP IVYTPTVGLA CSQYGHIFRR PKGLFISISD RGHVRSIVDN WPENHVKAVV V TDGERILG LGDLGVYGMG IPVGKLCLYT ACAGIRPDRC LPVCIDVGTD NIALLKDPFY MGLYQKRDRT QQYDDLIDEF MK AITDRYG RNTLIQFEDF GNHNAFRFLR KYREKYCTFN DDIQGTAAVA LAGLLAAQKV ISKPISEHKI LFLGAGEAAL GIA NLIVMS MVENGLSEQE AQKKIWMFDK YGLLVKGRKA KIDSYQEPFT HSAPESIPDT FEDAVNILKP STIIGVAGAG RLFT PDVIR AMASINERPV IFALSNPTAQ AECTAEEAYT LTEGRCLFAS GSPFGPVKLT DGRVFTPGQG NNVYIFPGVA LAVIL CNTR HISDSVFLEA AKALTSQLTD EELAQGRLYP PLANIQEVSI NIAIKVTEYL YANKMAFRYP EPEDKAKYVK ERTWRS EYD SLLPDVYEWP ESASSPPVIT E |
-Macromolecule #2: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
Macromolecule | Name: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / type: ligand / ID: 2 / Number of copies: 8 / Formula: NAD |
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Molecular weight | Theoretical: 663.425 Da |
Chemical component information | ![]() ChemComp-NAD: |
-Macromolecule #3: 4-[(3-carboxy-2-oxidanyl-naphthalen-1-yl)methyl]-3-oxidanyl-napht...
Macromolecule | Name: 4-[(3-carboxy-2-oxidanyl-naphthalen-1-yl)methyl]-3-oxidanyl-naphthalene-2-carboxylic acid type: ligand / ID: 3 / Number of copies: 4 / Formula: JBR |
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Molecular weight | Theoretical: 388.37 Da |
Chemical component information | ![]() ChemComp-JBR: |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |