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- EMDB-32462: Left PSI in the cyclic electron transport supercomplex NDH-PSI fr... -

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Basic information

Entry
Database: EMDB / ID: EMD-32462
TitleLeft PSI in the cyclic electron transport supercomplex NDH-PSI from Arabidopsis
Map data
Sample
  • Complex: Cylic electron transfer supercomplex from Arabidopsis
    • Protein or peptide: x 16 types
  • Ligand: x 11 types
Function / homology
Function and homology information


photosynthetic NADP+ reduction / photosystem I stabilization / chloroplast photosystem I / chloroplast stromal thylakoid / response to low light intensity stimulus / plastoglobule / chloroplast membrane / response to high light intensity / chloroplast thylakoid / photosynthesis, light harvesting in photosystem I ...photosynthetic NADP+ reduction / photosystem I stabilization / chloroplast photosystem I / chloroplast stromal thylakoid / response to low light intensity stimulus / plastoglobule / chloroplast membrane / response to high light intensity / chloroplast thylakoid / photosynthesis, light harvesting in photosystem I / chloroplast envelope / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / thylakoid / photosystem I / plastid / chlorophyll binding / chloroplast thylakoid membrane / response to light stimulus / photosynthesis / response to cold / chloroplast / 4 iron, 4 sulfur cluster binding / electron transfer activity / membrane => GO:0016020 / protein stabilization / protein domain specific binding / mRNA binding / magnesium ion binding / extracellular region / metal ion binding / nucleus / plasma membrane / cytosol
Similarity search - Function
4Fe-4S dicluster domain / Photosystem I PsaH, reaction centre subunit VI / Photosystem I reaction centre subunit VI / Photosystem I reaction center subunit V / Photosystem I reaction center subunit psaK, plant / Photosystem I reaction center subunit V/PsaK, plant / Photosystem I PsaG/PsaK domain, chloroplastic / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK ...4Fe-4S dicluster domain / Photosystem I PsaH, reaction centre subunit VI / Photosystem I reaction centre subunit VI / Photosystem I reaction center subunit V / Photosystem I reaction center subunit psaK, plant / Photosystem I reaction center subunit V/PsaK, plant / Photosystem I PsaG/PsaK domain, chloroplastic / Photosystem I reaction centre subunit PsaK superfamily / Photosystem I psaG and psaK proteins signature. / Photosystem I reaction center subunit V/PsaK / Photosystem I psaG / psaK / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I PsaD / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I, reaction centre subunit PsaD superfamily / Photosystem I reaction centre subunit IX / PsaJ / PsaD / Chlorophyll A-B binding protein, plant and chromista / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / Chlorophyll A-B binding protein / Chlorophyll A-B binding protein / Photosystem I protein PsaC / Photosystem I PsaA / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
Chlorophyll a-b binding protein 4, chloroplastic / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I reaction center subunit VIII / Photosystem I reaction center subunit IX / Photosystem I iron-sulfur center / Chlorophyll a-b binding protein 6, chloroplastic / Photosystem I chlorophyll a/b-binding protein 6, chloroplastic / Photosystem I reaction center subunit V, chloroplastic / Photosystem I reaction center subunit IV A, chloroplastic ...Chlorophyll a-b binding protein 4, chloroplastic / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I P700 chlorophyll a apoprotein A2 / Photosystem I reaction center subunit VIII / Photosystem I reaction center subunit IX / Photosystem I iron-sulfur center / Chlorophyll a-b binding protein 6, chloroplastic / Photosystem I chlorophyll a/b-binding protein 6, chloroplastic / Photosystem I reaction center subunit V, chloroplastic / Photosystem I reaction center subunit IV A, chloroplastic / Photosystem I reaction center subunit II-2, chloroplastic / Photosystem I reaction center subunit III, chloroplastic / Photosystem I reaction center subunit XI, chloroplastic / Photosystem I reaction center subunit psaK, chloroplastic / Photosystem I reaction center subunit VI-2, chloroplastic / Photosystem I chlorophyll a/b-binding protein 3-1, chloroplastic
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress) / thale cress (thale cress)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.25 Å
AuthorsPan X / Li M
Funding support China, 5 items
OrganizationGrant numberCountry
Ministry of Science and Technology (MoST, China)2017YFA0503702 China
Chinese Academy of SciencesXDB08020106 China
National Natural Science Foundation of China (NSFC)31930064 China
National Natural Science Foundation of China (NSFC)31970264 China
National Natural Science Foundation of China (NSFC)31770778 China
CitationJournal: Mol Plant / Year: 2022
Title: Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana.
Authors: Xiaodong Su / Duanfang Cao / Xiaowei Pan / Lifang Shi / Zhenfeng Liu / Luca Dall'Osto / Roberto Bassi / Xinzheng Zhang / Mei Li /
Abstract: Cyclic electron transport/flow (CET/CEF) in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency. A crucial CEF pathway is catalyzed by a membrane- ...Cyclic electron transport/flow (CET/CEF) in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency. A crucial CEF pathway is catalyzed by a membrane-embedded NADH dehydrogenase-like (NDH) complex that contains at least 29 protein subunits and associates with photosystem I (PSI) to form the NDH-PSI supercomplex. Here, we report the 3.9 Å resolution structure of the Arabidopsis thaliana NDH-PSI (AtNDH-PSI) supercomplex. We constructed structural models for 26 AtNDH subunits, among which 11 are unique to chloroplasts and stabilize the core part of the NDH complex. In the supercomplex, one NDH can bind up to two PSI-light-harvesting complex I (PSI-LHCI) complexes at both sides of its membrane arm. Two minor LHCIs, Lhca5 and Lhca6, each present in one PSI-LHCI, interact with NDH and contribute to supercomplex formation and stabilization. Collectively, our study reveals the structural details of the AtNDH-PSI supercomplex assembly and provides a molecular basis for further investigation of the regulatory mechanism of CEF in plants.
History
DepositionDec 26, 2021-
Header (metadata) releaseMar 16, 2022-
Map releaseMar 16, 2022-
UpdateMar 16, 2022-
Current statusMar 16, 2022Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7wfd
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7wfd
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_32462.png

Downloads & links

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Map

FileDownload / File: emd_32462.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.04 Å
Density
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.15325998 - 0.4218805
Average (Standard dev.)0.00030863905 (±0.0071013314)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-200-200-200
Dimensions400400400
Spacing400400400
CellA=B=C: 416.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.041.041.04
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z416.000416.000416.000
α/β/γ90.00090.00090.000
start NX/NY/NZ139118109
NX/NY/NZ123164187
MAP C/R/S123
start NC/NR/NS-200-200-200
NC/NR/NS400400400
D min/max/mean-0.1530.4220.000

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Supplemental data

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Sample components

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Entire : Cylic electron transfer supercomplex from Arabidopsis

EntireName: Cylic electron transfer supercomplex from Arabidopsis
Components
  • Complex: Cylic electron transfer supercomplex from Arabidopsis
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A1
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2
    • Protein or peptide: Photosystem I iron-sulfur center
    • Protein or peptide: Photosystem I reaction center subunit II-2, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit IV A, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit III, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit V, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit VI-2, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit VIII
    • Protein or peptide: Photosystem I reaction center subunit IX
    • Protein or peptide: Photosystem I reaction center subunit psaK, chloroplastic
    • Protein or peptide: Photosystem I reaction center subunit XI, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein 6, chloroplastic
    • Protein or peptide: Photosystem I chlorophyll a/b-binding protein 3-1, chloroplastic
    • Protein or peptide: Chlorophyll a-b binding protein 4, chloroplastic
    • Protein or peptide: Photosystem I chlorophyll a/b-binding protein 6, chloroplastic
  • Ligand: CHLOROPHYLL A
  • Ligand: PHYLLOQUINONEPhytomenadione
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: BETA-CAROTENEΒ-Carotene
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: DODECYL-ALPHA-D-MALTOSIDE
  • Ligand: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
  • Ligand: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: CHLOROPHYLL B
  • Ligand: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

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Supramolecule #1: Cylic electron transfer supercomplex from Arabidopsis

SupramoleculeName: Cylic electron transfer supercomplex from Arabidopsis / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#16
Source (natural)Organism: Arabidopsis thaliana (thale cress)

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Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 83.315367 KDa
SequenceString: MIIRSPEPEV KILVDRDPIK TSFEEWAKPG HFSRTIAKGP DTTTWIWNLH ADAHDFDSHT SDLEEISRKV FSAHFGQLSI IFLWLSGMY FHGARFSNYE AWLSDPTHIG PSAQVVWPIV GQEILNGDVG GGFRGIQITS GFFQIWRASG ITSELQLYCT A IGALVFAA ...String:
MIIRSPEPEV KILVDRDPIK TSFEEWAKPG HFSRTIAKGP DTTTWIWNLH ADAHDFDSHT SDLEEISRKV FSAHFGQLSI IFLWLSGMY FHGARFSNYE AWLSDPTHIG PSAQVVWPIV GQEILNGDVG GGFRGIQITS GFFQIWRASG ITSELQLYCT A IGALVFAA LMLFAGWFHY HKAAPKLAWF QDVESMLNHH LAGLLGLGSL SWAGHQVHVS LPINQFLNAG VDPKEIPLPH EF ILNRDLL AQLYPSFAEG ATPFFTLNWS KYSEFLTFRG GLDPVTGGLW LTDIAHHHLA IAILFLIAGH MYRTNWGIGH GIK DILEAH KGPFTGQGHK GLYEILTTSW HAQLSLNLAM LGSLTIIVAH HMYSMPPYPY LATDYATQLS LFTHHMWIGG FLIV GAAAH AAIFMVRDYD PTNRYNDLLD RVLRHRDAII SHLNWVCIFL GFHSFGLYIH NDTMSALGRP QDMFSDTAIQ LQPVF AQWI QNTHALAPGV TAPGETASTS LTWGGGELVA VGGKVALLPI PLGTADFLVH HIHAFTIHVT VLILLKGVLF ARSSRL IPD KANLGFRFPC DGPGRGGTCQ VSAWDHVFLG LFWMYNAISV VIFHFSWKMQ SDVWGSISDQ GVVTHITGGN FAQSSIT IN GWLRDFLWAQ ASQVIQSYGS SLSAYGLFFL GAHFVWAFSL MFLFSGRGYW QELIESIVWA HNKLKVAPAT QPRALSII Q GRAVGVTHYL LGGIATTWAF FLARIIAVG

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Macromolecule #2: Photosystem I P700 chlorophyll a apoprotein A2

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 82.555883 KDa
SequenceString: MALRFPRFSQ GLAQDPTTRR IWFGIATAHD FESHDDITEE RLYQNIFASH FGQLAIIFLW TSGNLFHVAW QGNFETWVQD PLHVRPIAH AIWDPHFGQP AVEAFTRGGA LGPVNIAYSG VYQWWYTIGL RTNEDLYTGA LFLLFLSALS LIGGWLHLQP K WKPRVSWF ...String:
MALRFPRFSQ GLAQDPTTRR IWFGIATAHD FESHDDITEE RLYQNIFASH FGQLAIIFLW TSGNLFHVAW QGNFETWVQD PLHVRPIAH AIWDPHFGQP AVEAFTRGGA LGPVNIAYSG VYQWWYTIGL RTNEDLYTGA LFLLFLSALS LIGGWLHLQP K WKPRVSWF KNAESRLNHH LSGLFGVSSL AWTGHLVHVA IPASRGEYVR WNNFLNVLPH PQGLGPLFTG QWNLYAQNPD SS SHLFGTS QGSGTAILTL LGGFHPQTQS LWLTDMAHHH LAIAILFLIA GHMYRTNFGI GHSIKDLLEA HIPPGGRLGR GHK GLYDTI NNSIHFQLGL ALASLGVITS LVAQHMYSLP AYAFIAQDFT TQAALYTHHQ YIAGFIMTGA FAHGAIFFIR DYNP EQNED NVLARMLDHK EAIISHLSWA SLFLGFHTLG LYVHNDVMLA FGTPEKQILI EPIFAQWIQS AHGKTSYGFD VLLSS TSGP AFNAGRSIWL PGWLNAINEN SNSLFLTIGP GDFLVHHAIA LGLHTTTLIL VKGALDARGS KLMPDKKDFG YSFPCD GPG RGGTCDISAW DAFYLAVFWM LNTIGWVTFY WHWKHITLWQ GNVSQFNESS TYLMGWLRDY LWLNSSQLIN GYNPFGM NS LSVWAWMFLF GHLVWATGFM FLISWRGYWQ ELIETLAWAH ERTPLANLIR WKDKPVALSI VQARLVGLAH FSVGYIFT Y AAFLIASTSG KFG

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Macromolecule #3: Photosystem I iron-sulfur center

MacromoleculeName: Photosystem I iron-sulfur center / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 9.049509 KDa
SequenceString:
MSHSVKIYDT CIGCTQCVRA CPTDVLEMIP WDGCKAKQIA SAPRTEDCVG CKRCESACPT DFLSVRVYLW HETTRSMGLA Y

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Macromolecule #4: Photosystem I reaction center subunit II-2, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit II-2, chloroplastic
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 22.336598 KDa
SequenceString: MATQAAGIFS PAITTTTSAV KKLHLFSSSH RPKSLSFTKT AIRAEKTESS SAAPAVKEAP VGFTPPQLDP NTPSPIFAGS TGGLLRKAQ VEEFYVITWN SPKEQIFEMP TGGAAIMREG PNLLKLARKE QCLALGTRLR SKYKITYQFY RVFPNGEVQY L HPKDGVYP ...String:
MATQAAGIFS PAITTTTSAV KKLHLFSSSH RPKSLSFTKT AIRAEKTESS SAAPAVKEAP VGFTPPQLDP NTPSPIFAGS TGGLLRKAQ VEEFYVITWN SPKEQIFEMP TGGAAIMREG PNLLKLARKE QCLALGTRLR SKYKITYQFY RVFPNGEVQY L HPKDGVYP EKANPGREGV GLNMRSIGKN VSPIEVKFTG KQSYDL

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Macromolecule #5: Photosystem I reaction center subunit IV A, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit IV A, chloroplastic
type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 14.984955 KDa
SequenceString:
MAMTTASTVF VLPANVTSVA GASSSRSSVS FLPMRNAGSR LVVRAAEDPA PASSSSKDSP AAAAAPDGAT ATKPKPPPIG PKRGSKVKI LRRESYWFKN VGSVVAVDQD PKTRYPVVVR FAKVNYANIS TNNYALDEVE EVAA

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Macromolecule #6: Photosystem I reaction center subunit III, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit III, chloroplastic
type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 24.203125 KDa
SequenceString: MSLTIPANLV LNPRSNKSLT QSVPKSSARF VCSDDKSSSS TPQSMKAFSA AVALSSILLS APMPAVADIS GLTPCKDSKQ FAKREKQQI KKLESSLKLY APESAPALAL NAQIEKTKRR FDNYGKYGLL CGSDGLPHLI VNGDQRHWGE FITPGILFLY I AGWIGWVG ...String:
MSLTIPANLV LNPRSNKSLT QSVPKSSARF VCSDDKSSSS TPQSMKAFSA AVALSSILLS APMPAVADIS GLTPCKDSKQ FAKREKQQI KKLESSLKLY APESAPALAL NAQIEKTKRR FDNYGKYGLL CGSDGLPHLI VNGDQRHWGE FITPGILFLY I AGWIGWVG RSYLIAISGE KKPAMKEIII DVPLASRIIF RGFIWPVAAY REFLNGDLIA KDV

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Macromolecule #7: Photosystem I reaction center subunit V, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit V, chloroplastic
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 17.103271 KDa
SequenceString:
MATSASALLS PTTFSTAISH KNPNSISFHG LRPLRLGGSS SALPKLSTTG RKSSSAVVRA ELSPSIVISL STGLSLFLGR FVFFNFQRE NVAKQGLPEQ NGKTHFEAGD DRAKEYVSLL KSNDPIGFNI VDVLAWGSIG HIVAYYILAT SSNGYDPSFF G

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Macromolecule #8: Photosystem I reaction center subunit VI-2, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit VI-2, chloroplastic
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 15.291522 KDa
SequenceString:
MASFATIAAV QPSAAVKGLG GSSLAGAKLF IKPSRQSFKT KSTRAGAVVA KYGDKSVYFD LEDLGNTTGQ WDVYGSDAPS PYNPLQSKF FETFAAPFTK RGLLLKFLIL GGGSLLTYVS ANSTGDVLPI KRGPQEPPKL GPRGKL

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Macromolecule #9: Photosystem I reaction center subunit VIII

MacromoleculeName: Photosystem I reaction center subunit VIII / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 4.137024 KDa
SequenceString:
MTTFNNLPSI FVPLVGLVFP AIAMASLFLH IQKNKIF

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Macromolecule #10: Photosystem I reaction center subunit IX

MacromoleculeName: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 5.011897 KDa
SequenceString:
MRDLKTYLSV APVLSTLWFG SLAGLLIEIN RLFPDALTFP FFSF

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Macromolecule #11: Photosystem I reaction center subunit psaK, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit psaK, chloroplastic
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 13.219431 KDa
SequenceString:
MASTMMTTLP QFNGLRATKI SAAPVQGLAS VQPMRRKGNG ALGAKCDFIG SSTNLIMVTS TTLMLFAGRF GLAPSANRKA TAGLRLEAR DSGLQTGDPA GFTLADTLAC GTVGHIIGVG VVLGLKNIGA I

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Macromolecule #12: Photosystem I reaction center subunit XI, chloroplastic

MacromoleculeName: Photosystem I reaction center subunit XI, chloroplastic
type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 23.070557 KDa
SequenceString: MAASASPMAS QLRSSFSSAS LSQRLAVPKG ISGAPFGVSP TKRVSSFTVR AVKSDKTTFQ VVQPINGDPF IGSLETPVTS SPLIAWYLS NLPGYRTAVN PLLRGVEVGL AHGFFLVGPF VKAGPLRNTA YAGSAGSLAA AGLVVILSMC LTIYGISSFK E GEPSIAPS ...String:
MAASASPMAS QLRSSFSSAS LSQRLAVPKG ISGAPFGVSP TKRVSSFTVR AVKSDKTTFQ VVQPINGDPF IGSLETPVTS SPLIAWYLS NLPGYRTAVN PLLRGVEVGL AHGFFLVGPF VKAGPLRNTA YAGSAGSLAA AGLVVILSMC LTIYGISSFK E GEPSIAPS LTLTGRKKQP DQLQTADGWA KFTGGFFFGG ISGVTWAYFL LYVLDLPYFV K

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Macromolecule #13: Chlorophyll a-b binding protein 6, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein 6, chloroplastic / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 26.021895 KDa
SequenceString: MASNSLMSCG IAAVYPSLLS SSKSKFVSAG VPLPNAGNVG RIRMAAHWMP GEPRPAYLDG SAPGDFGFDP LGLGEVPANL ERYKESELI HCRWAMLAVP GILVPEALGY GNWVKAQEWA ALPGGQATYL GNPVPWGTLP TILAIEFLAI AFVEHQRSME K DPEKKKYP ...String:
MASNSLMSCG IAAVYPSLLS SSKSKFVSAG VPLPNAGNVG RIRMAAHWMP GEPRPAYLDG SAPGDFGFDP LGLGEVPANL ERYKESELI HCRWAMLAVP GILVPEALGY GNWVKAQEWA ALPGGQATYL GNPVPWGTLP TILAIEFLAI AFVEHQRSME K DPEKKKYP GGAFDPLGYS KDPKKLEELK VKEIKNGRLA LLAFVGFCVQ QSAYPGTGPL ENLATHLADP WHNNIGDIVI PF N

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Macromolecule #14: Photosystem I chlorophyll a/b-binding protein 3-1, chloroplastic

MacromoleculeName: Photosystem I chlorophyll a/b-binding protein 3-1, chloroplastic
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 29.206311 KDa
SequenceString: MAAQALVSSS LTSSVQTARQ IFGSKPVASA SQKKSSFVVK AAATPPVKQG ANRPLWFASS QSLSYLDGSL PGDYGFDPLG LSDPEGTGG FIEPRWLAYG EIINGRFAML GAAGAIAPEI LGKAGLIPAE TALPWFQTGV IPPAGTYTYW ADNYTLFVLE M ALMGFAEH ...String:
MAAQALVSSS LTSSVQTARQ IFGSKPVASA SQKKSSFVVK AAATPPVKQG ANRPLWFASS QSLSYLDGSL PGDYGFDPLG LSDPEGTGG FIEPRWLAYG EIINGRFAML GAAGAIAPEI LGKAGLIPAE TALPWFQTGV IPPAGTYTYW ADNYTLFVLE M ALMGFAEH RRLQDWYNPG SMGKQYFLGL EKGLAGSGNP AYPGGPFFNP LGFGKDEKSL KELKLKEVKN GRLAMLAILG YF IQGLVTG VGPYQNLLDH LADPVNNNVL TSLKFH

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Macromolecule #15: Chlorophyll a-b binding protein 4, chloroplastic

MacromoleculeName: Chlorophyll a-b binding protein 4, chloroplastic / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 27.760461 KDa
SequenceString: MATVTTHASA SIFRPCTSKP RFLTGSSGRL NRDLSFTSIG SSAKTSSFKV EAKKGEWLPG LASPDYLTGS LAGDNGFDPL GLAEDPENL KWFVQAELVN GRWAMLGVAG MLLPEVFTKI GIINVPEWYD AGKEQYFASS STLFVIEFIL FHYVEIRRWQ D IKNPGSVN ...String:
MATVTTHASA SIFRPCTSKP RFLTGSSGRL NRDLSFTSIG SSAKTSSFKV EAKKGEWLPG LASPDYLTGS LAGDNGFDPL GLAEDPENL KWFVQAELVN GRWAMLGVAG MLLPEVFTKI GIINVPEWYD AGKEQYFASS STLFVIEFIL FHYVEIRRWQ D IKNPGSVN QDPIFKQYSL PKGEVGYPGG IFNPLNFAPT QEAKEKELAN GRLAMLAFLG FVVQHNVTGK GPFENLLQHL SD PWHNTIV QTFN

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Macromolecule #16: Photosystem I chlorophyll a/b-binding protein 6, chloroplastic

MacromoleculeName: Photosystem I chlorophyll a/b-binding protein 6, chloroplastic
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: thale cress (thale cress)
Molecular weightTheoretical: 29.969236 KDa
SequenceString: MAFAIASALT STLTLSTSRV QNPTQRRPHV ASTSSTGGRL MRERLVVVRA GKEVSSVCEP LPPDRPLWFP GSSPPEWLDG SLPGDFGFD PLGLGSDPDT LKWFAQAELI HSRWAMLAVT GIIIPECLER LGFIENFSWY DAGSREYFAD STTLFVAQMV L MGWAEGRR ...String:
MAFAIASALT STLTLSTSRV QNPTQRRPHV ASTSSTGGRL MRERLVVVRA GKEVSSVCEP LPPDRPLWFP GSSPPEWLDG SLPGDFGFD PLGLGSDPDT LKWFAQAELI HSRWAMLAVT GIIIPECLER LGFIENFSWY DAGSREYFAD STTLFVAQMV L MGWAEGRR WADLIKPGSV DIEPKYPHKV NPKPDVGYPG GLWFDFMMWG RGSPEPVMVL RTKEIKNGRL AMLAFLGFCF QA TYTSQDP IENLMAHLAD PGHCNVFSAF TSH

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Macromolecule #17: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 17 / Number of copies: 143 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

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Macromolecule #18: PHYLLOQUINONE

MacromoleculeName: PHYLLOQUINONE / type: ligand / ID: 18 / Number of copies: 2 / Formula: PQN
Molecular weightTheoretical: 450.696 Da
Chemical component information

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

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Macromolecule #19: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 19 / Number of copies: 8 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #20: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 20 / Number of copies: 26 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

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Macromolecule #21: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 21 / Number of copies: 3 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

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Macromolecule #22: DODECYL-ALPHA-D-MALTOSIDE

MacromoleculeName: DODECYL-ALPHA-D-MALTOSIDE / type: ligand / ID: 22 / Number of copies: 5 / Formula: LMU
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

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Macromolecule #23: DIGALACTOSYL DIACYL GLYCEROL (DGDG)

MacromoleculeName: DIGALACTOSYL DIACYL GLYCEROL (DGDG) / type: ligand / ID: 23 / Number of copies: 1 / Formula: DGD
Molecular weightTheoretical: 949.299 Da
Chemical component information

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

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Macromolecule #24: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL

MacromoleculeName: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 24 / Number of copies: 5 / Formula: LUT
Molecular weightTheoretical: 568.871 Da
Chemical component information

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

+
Macromolecule #25: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 25 / Number of copies: 3 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

+
Macromolecule #26: CHLOROPHYLL B

MacromoleculeName: CHLOROPHYLL B / type: ligand / ID: 26 / Number of copies: 11 / Formula: CHL
Molecular weightTheoretical: 907.472 Da
Chemical component information

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

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Macromolecule #27: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...

MacromoleculeName: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
type: ligand / ID: 27 / Number of copies: 4 / Formula: XAT
Molecular weightTheoretical: 600.87 Da
Chemical component information

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 60.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5zji

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.25 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 136022

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