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Structure paper

TitleSupramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana.
Journal, issue, pagesMol Plant, Vol. 15, Issue 3, Page 454-467, Year 2022
Publish dateMar 7, 2022
AuthorsXiaodong Su / Duanfang Cao / Xiaowei Pan / Lifang Shi / Zhenfeng Liu / Luca Dall'Osto / Roberto Bassi / Xinzheng Zhang / Mei Li /
PubMed AbstractCyclic electron transport/flow (CET/CEF) in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency. A crucial CEF pathway is catalyzed by a membrane- ...Cyclic electron transport/flow (CET/CEF) in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency. A crucial CEF pathway is catalyzed by a membrane-embedded NADH dehydrogenase-like (NDH) complex that contains at least 29 protein subunits and associates with photosystem I (PSI) to form the NDH-PSI supercomplex. Here, we report the 3.9 Å resolution structure of the Arabidopsis thaliana NDH-PSI (AtNDH-PSI) supercomplex. We constructed structural models for 26 AtNDH subunits, among which 11 are unique to chloroplasts and stabilize the core part of the NDH complex. In the supercomplex, one NDH can bind up to two PSI-light-harvesting complex I (PSI-LHCI) complexes at both sides of its membrane arm. Two minor LHCIs, Lhca5 and Lhca6, each present in one PSI-LHCI, interact with NDH and contribute to supercomplex formation and stabilization. Collectively, our study reveals the structural details of the AtNDH-PSI supercomplex assembly and provides a molecular basis for further investigation of the regulatory mechanism of CEF in plants.
External linksMol Plant / PubMed:35123031
MethodsEM (single particle)
Resolution3.25 - 4.33 Å
Structure data

32462

7wfd

EMDB-32462, PDB-7wfd:
Left PSI in the cyclic electron transport supercomplex NDH-PSI from Arabidopsis
Method: EM (single particle) / Resolution: 3.25 Å

32463

7wfe

EMDB-32463, PDB-7wfe:
Right PSI in the cyclic electron transfer supercomplex NDH-PSI from Arabidopsis
Method: EM (single particle) / Resolution: 3.25 Å

32464

7wff

EMDB-32464, PDB-7wff:
Subcomplexes B,M and L in the Cylic electron transfer supercomplex NDH-PSI from Arabidopsis
Method: EM (single particle) / Resolution: 3.59 Å

32465

7wfg

EMDB-32465, PDB-7wfg:
Subcomplexes A and E in NDH complex from Arabidopsis
Method: EM (single particle) / Resolution: 4.33 Å

32477

7wg5

EMDB-32477, PDB-7wg5:
Cyclic electron transport supercomplex NDH-PSI from Arabidopsis
Method: EM (single particle) / Resolution: 3.89 Å

Chemicals

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-LMU:
DODECYL-ALPHA-D-MALTOSIDE / detergent*YM

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

Source
  • arabidopsis thaliana (thale cress)
  • thale cress (thale cress)
KeywordsELECTRON TRANSPORT / Supercomplex / PSI / NDH-PSI / plant / Arabidopsis / cyclic electron transport / Cylic electron trasport / Subcomplex B / subcomplex M / subcomplex L / Arabidopisis / cyclic electron transport supercomplex / Subcomplex A / subcomplex E

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