[English] 日本語
Yorodumi- EMDB-1555: Molecular Architecture of the 'stressosome', a signal transduction hub -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-1555 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Molecular Architecture of the 'stressosome', a signal transduction hub | |||||||||
Map data | RsbR RsbS binary 'Stressosome' complex | |||||||||
Sample |
| |||||||||
Keywords | RsbR / RsbS / Stressosome / sigmaB / RsbT / stress response / bacillus | |||||||||
Biological species | Bacillus subtilis (bacteria) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 8.0 Å | |||||||||
Authors | Marles-Wright J / Grant T / Delumeau O / van Duinen G / Firbank SJ / Lewis PJ / Murray JW / Newman JA / Quin MB / Race PR ...Marles-Wright J / Grant T / Delumeau O / van Duinen G / Firbank SJ / Lewis PJ / Murray JW / Newman JA / Quin MB / Race PR / Rohou A / Tichelaar W / van Heel M / Lewis RJ | |||||||||
Citation | Journal: Science / Year: 2008 Title: Molecular architecture of the "stressosome," a signal integration and transduction hub. Authors: Jon Marles-Wright / Tim Grant / Olivier Delumeau / Gijs van Duinen / Susan J Firbank / Peter J Lewis / James W Murray / Joseph A Newman / Maureen B Quin / Paul R Race / Alexis Rohou / Willem ...Authors: Jon Marles-Wright / Tim Grant / Olivier Delumeau / Gijs van Duinen / Susan J Firbank / Peter J Lewis / James W Murray / Joseph A Newman / Maureen B Quin / Paul R Race / Alexis Rohou / Willem Tichelaar / Marin van Heel / Richard J Lewis / Abstract: A commonly used strategy by microorganisms to survive multiple stresses involves a signal transduction cascade that increases the expression of stress-responsive genes. Stress signals can be ...A commonly used strategy by microorganisms to survive multiple stresses involves a signal transduction cascade that increases the expression of stress-responsive genes. Stress signals can be integrated by a multiprotein signaling hub that responds to various signals to effect a single outcome. We obtained a medium-resolution cryo-electron microscopy reconstruction of the 1.8-megadalton "stressosome" from Bacillus subtilis. Fitting known crystal structures of components into this reconstruction gave a pseudoatomic structure, which had a virus capsid-like core with sensory extensions. We suggest that the different sensory extensions respond to different signals, whereas the conserved domains in the core integrate the varied signals. The architecture of the stressosome provides the potential for cooperativity, suggesting that the response could be tuned dependent on the magnitude of chemophysical insult. | |||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_1555.map.gz | 5.5 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-1555-v30.xml emd-1555.xml | 7.8 KB 7.8 KB | Display Display | EMDB header |
Images | emd1555.png | 272.9 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-1555 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1555 | HTTPS FTP |
-Validation report
Summary document | emd_1555_validation.pdf.gz | 234.2 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_1555_full_validation.pdf.gz | 233.3 KB | Display | |
Data in XML | emd_1555_validation.xml.gz | 6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1555 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1555 | HTTPS FTP |
-Related structure data
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|
-Map
File | Download / File: emd_1555.map.gz / Format: CCP4 / Size: 10.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | RsbR RsbS binary 'Stressosome' complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.54 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Sample components
-Entire : RsbR RsbS binary complex
Entire | Name: RsbR RsbS binary complex |
---|---|
Components |
|
-Supramolecule #1000: RsbR RsbS binary complex
Supramolecule | Name: RsbR RsbS binary complex / type: sample / ID: 1000 / Number unique components: 1 |
---|
-Macromolecule #1: RsbR RsbS binary complex
Macromolecule | Name: RsbR RsbS binary complex / type: protein_or_peptide / ID: 1 / Name.synonym: Stressosome / Recombinant expression: Yes |
---|---|
Source (natural) | Organism: Bacillus subtilis (bacteria) |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Vitrification | Cryogen name: ETHANE / Instrument: OTHER / Details: Vitrification instrument: Vitrobot |
---|
-Electron microscopy
Microscope | FEI/PHILIPS CM200FEG |
---|---|
Image recording | Digitization - Scanner: NIKON SUPER COOLSCAN 9000 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Sample stage | Specimen holder: Side entry liquid nitrogen-cooled cryo specimen holder Specimen holder model: GATAN LIQUID NITROGEN |
-Image processing
Final reconstruction | Applied symmetry - Point group: D2 (2x2 fold dihedral) / Resolution.type: BY AUTHOR / Resolution: 8.0 Å / Resolution method: OTHER / Software - Name: IMAGIC |
---|