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    - EMDB-1552: Molecular Architecture of the 'stressosome', a signal transduction hub -

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    Basic information

    Entry
    Database: EMDB / ID: 1552
    TitleMolecular Architecture of the 'stressosome', a signal transduction hub
    KeywordsRsbR / RsbS / Stressosome / sigmaB / RsbT / stress response / bacillus
    SampleRsbR146-274RsbS stressosome core
    SourceBacillus subtilis / bacteria /
    Map dataRsbR146-274RsbS stressosome core with imposed icosahedral symmetry
    Methodsingle particle (icosahedral) reconstruction, at 6.5 A resolution
    AuthorsMarles-Wright J / Grant T / Delumeau O / van Duinen G / Firbank SJ / Lewis PJ / Murray JW / Newman JA / Quin MB / Race PR / Rohou A / Tichelaar W / van Heel M / Lewis RJ
    CitationScience, 2008, 322, 92-96

    Science, 2008, 322, 92-96 StrPapers
    Molecular architecture of the "stressosome," a signal integration and transduction hub.
    Jon Marles-Wright / Tim Grant / Olivier Delumeau / Gijs van Duinen / Susan J Firbank / Peter J Lewis / James W Murray / Joseph A Newman / Maureen B Quin / Paul R Race / Alexis Rohou / Willem Tichelaar / Marin van Heel / Richard J Lewis

    DateDeposition: Sep 8, 2008 / Header (metadata) release: Sep 11, 2008 / Map release: Apr 16, 2009 / Last update: Sep 8, 2008

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    Structure visualization

    Movie
    • Surface view with section colored by density value
    • Surface level: 30
    • Imaged by UCSF CHIMERA
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    • Surface view colored by radius
    • Surface level: 30
    • Imaged by UCSF CHIMERA
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    Map

    Fileemd_1552.map.gz (map file in CCP4 format, 3908 KB)
    Projections & slicesSize of images:
    AxesZ (Sec.)Y (Row.)X (Col.)
    100 pix
    2.54 A/pix
    = 254. A
    100 pix
    2.54 A/pix
    = 254. A
    100 pix
    2.54 A/pix
    = 254. A

    Surface

    Projections

    Slices (1/3)

    Slices (1/2)

    Slices (2/3)

    Images are generated by Spider package.

    Voxel sizeX=Y=Z: 2.54 A
    Density
    Contour Level:14, 30 (movie #1):
    Minimum - Maximum-104.048 - 109.155
    Average (Standard dev.)2.38557e-10 (10)
    Details

    EMDB XML:

    Space Group Number1
    Map Geometry
    Axis orderXYZ
    Dimensions100100100
    Origin-50-49-50
    Limit495049
    Spacing100100100
    CellA=B=C: 254 A
    Alpha=beta=gamma: 90 deg.

    CCP4 map header:

    modeImage stored as Reals
    A/pix X/Y/Z2.542.542.54
    M x/y/z100100100
    origin x/y/z0.0000.0000.000
    length x/y/z254.000254.000254.000
    alpha/beta/gamma90.00090.00090.000
    start NX/NY/NZ-81-81-81
    NX/NY/NZ160160160
    MAP C/R/S123
    start NC/NR/NS-49-50-50
    NC/NR/NS100100100
    D min/max/mean-104.048109.1550.000

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    Supplemental data

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    Sample components

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    Entire RsbR146-274RsbS stressosome core

    EntireName: RsbR146-274RsbS stressosome core / Number of components: 1

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    Component #1: protein, RsbR146-274RsbS

    ProteinName: RsbR146-274RsbS / a.k.a: Stressosome core / Recombinant expression: Yes
    SourceSpecies: Bacillus subtilis / bacteria /

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    Experimental details

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    Sample preparation

    Specimen stateparticle
    VitrificationInstrument: NONE / Cryogen name: ETHANE

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    Electron microscopy imaging

    ImagingMicroscope: FEI/PHILIPS CM200FEG
    Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: OTHER
    LensImaging mode: OTHER
    Specimen HolderHolder: Side entry liquid nitrogen-cooled cryo specimen holder
    Model: GATAN LIQUID NITROGEN

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    Image processing

    ProcessingMethod: single particle (icosahedral) reconstruction / Applied symmetry: I (icosahedral)
    3D reconstructionSoftware: IMAGIC / Resolution: 6.5 A / Resolution method: 1/2-bit

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    Atomic model buiding

    Modeling #1Refinement space: REAL
    Details: Homology models for R and S were made from 2VY9 and fitted
    Input PDB model: 2VY9

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