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- EMDB-12632: Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound b... -

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Basic information

Entry
Database: EMDB / ID: EMD-12632
TitleMammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S.
Map data
Sample
  • Complex: Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S.
    • Protein or peptide: x 79 types
    • RNA: x 5 types
  • Ligand: x 4 types
Function / homology
Function and homology information


L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Formation of a pool of free 40S subunits / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Translation initiation complex formation / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition ...L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Formation of a pool of free 40S subunits / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Translation initiation complex formation / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / Major pathway of rRNA processing in the nucleolus and cytosol / GTP hydrolysis and joining of the 60S ribosomal subunit / translation termination factor activity / Translesion synthesis by REV1 / Recognition of DNA damage by PCNA-containing replication complex / Translesion Synthesis by POLH / Downregulation of ERBB4 signaling / Spry regulation of FGF signaling / Downregulation of ERBB2:ERBB3 signaling / NOD1/2 Signaling Pathway / APC/C:Cdc20 mediated degradation of Cyclin B / SCF-beta-TrCP mediated degradation of Emi1 / APC-Cdc20 mediated degradation of Nek2A / EGFR downregulation / TCF dependent signaling in response to WNT / NRIF signals cell death from the nucleus / p75NTR recruits signalling complexes / NF-kB is activated and signals survival / Activated NOTCH1 Transmits Signal to the Nucleus / Downregulation of TGF-beta receptor signaling / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / Downregulation of SMAD2/3:SMAD4 transcriptional activity / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / Senescence-Associated Secretory Phenotype (SASP) / Regulation of innate immune responses to cytosolic DNA / activated TAK1 mediates p38 MAPK activation / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / Regulation of FZD by ubiquitination / PINK1-PRKN Mediated Mitophagy / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Regulation of TNFR1 signaling / TNFR1-induced NF-kappa-B signaling pathway / Translesion synthesis by POLK / Translesion synthesis by POLI / Regulation of necroptotic cell death / MAP3K8 (TPL2)-dependent MAPK1/3 activation / HDR through Homologous Recombination (HRR) / Josephin domain DUBs / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / DNA Damage Recognition in GG-NER / Formation of Incision Complex in GG-NER / Gap-filling DNA repair synthesis and ligation in GG-NER / Dual Incision in GG-NER / Fanconi Anemia Pathway / Regulation of TP53 Activity through Phosphorylation / Regulation of TP53 Degradation / Regulation of TP53 Activity through Methylation / Negative regulation of MET activity / Cyclin D associated events in G1 / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Downregulation of ERBB2 signaling / E3 ubiquitin ligases ubiquitinate target proteins / Regulation of PTEN localization / ER Quality Control Compartment (ERQC) / Regulation of expression of SLITs and ROBOs / Interferon alpha/beta signaling / Endosomal Sorting Complex Required For Transport (ESCRT) / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / IKK complex recruitment mediated by RIP1 / IRAK2 mediated activation of TAK1 complex / TRAF6-mediated induction of TAK1 complex within TLR4 complex / Alpha-protein kinase 1 signaling pathway / RAS processing / Pexophagy / Inactivation of CSF3 (G-CSF) signaling / Negative regulation of FLT3 / Regulation of BACH1 activity / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / Regulation of NF-kappa B signaling / Termination of translesion DNA synthesis / Ovarian tumor domain proteases / Negative regulators of DDX58/IFIH1 signaling / Negative regulation of FGFR1 signaling / Negative regulation of FGFR2 signaling / Negative regulation of FGFR3 signaling / Negative regulation of FGFR4 signaling / Negative regulation of MAPK pathway / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Iron uptake and transport / Deactivation of the beta-catenin transactivating complex / Metalloprotease DUBs / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / Activation of NF-kappaB in B cells / L13a-mediated translational silencing of Ceruloplasmin expression / Autodegradation of Cdh1 by Cdh1:APC/C / APC/C:Cdc20 mediated degradation of Securin / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / Cdc20:Phospho-APC/C mediated degradation of Cyclin A
Similarity search - Function
Peptide chain release factor eRF1/aRF1 / eRF1, domain 1 / eRF1 domain 2 / eRF1 domain 2 / eRF1 domain 1 / eRF1 domain 1/Pelota-like / eRF1 domain 3 / eRF1, domain 2 superfamily / eRF1 domain 3 / eRF1_1 ...Peptide chain release factor eRF1/aRF1 / eRF1, domain 1 / eRF1 domain 2 / eRF1 domain 2 / eRF1 domain 1 / eRF1 domain 1/Pelota-like / eRF1 domain 3 / eRF1, domain 2 superfamily / eRF1 domain 3 / eRF1_1 / : / Ribosomal protein L2, archaeal-type / : / metallochaperone-like domain / TRASH domain / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S21e signature. / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein L24e, conserved site / Ribosomal S17 / 40S Ribosomal protein S10 / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein L19, eukaryotic / Ribosomal protein S17e signature. / Ribosomal protein L10e signature. / Ribosomal protein L27e signature. / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L19/L19e conserved site / Ribosomal protein L39e, conserved site / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L19e signature. / Ribosomal protein L24e signature. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L34e signature. / Ribosomal protein L6e signature. / Ribosomal protein L30e signature 1. / Ribosomal protein L30e signature 2. / 60S ribosomal protein L19 / Ribosomal protein L39e signature. / Ribosomal protein L39e / Ribosomal protein L24e-related / Ribosomal protein L39e domain superfamily / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L35Ae signature. / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal L39 protein / Ribosomal protein L19e / Ribosomal protein S28e signature. / Ribosomal protein L24e / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L32e signature. / Ribosomal protein L6 signature 2. / Ribosomal protein L1e signature. / Ribosomal protein L15e signature. / Ribosomal protein L21e signature. / Ribosomal protein L37e signature. / 50S ribosomal protein L30e-like / Ubiquitin conserved site / Ubiquitin domain / Ubiquitin domain signature. / Ribosomal protein L23 signature. / Ribosomal protein L30 signature. / Ribosomal protein S3 signature. / Ubiquitin family / Ribosomal protein L5 signature. / Ribosomal protein L2, conserved site / Ribosomal protein L2 signature. / Ribosomal protein L29 signature. / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S4/S9, N-terminal / Ribosomal protein S4, conserved site / Type-2 KH domain profile. / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S4/S9 / Ribosomal protein S19 signature. / Ubiquitin homologues / Ribosomal protein L2, domain 3
Similarity search - Domain/homology
Large ribosomal subunit protein eL24 / Small ribosomal subunit protein eS32 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein eL20 / 40S ribosomal protein S10 / Large ribosomal subunit protein uL16 / Epididymis tissue sperm binding protein Li 3a / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 ...Large ribosomal subunit protein eL24 / Small ribosomal subunit protein eS32 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein eL20 / 40S ribosomal protein S10 / Large ribosomal subunit protein uL16 / Epididymis tissue sperm binding protein Li 3a / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL14 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein eL15 / Small ribosomal subunit protein uS11 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS28 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL27 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL34 / Eukaryotic peptide chain release factor subunit 1 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / 60S ribosomal protein L8 / Large ribosomal subunit protein eL19 / 40S ribosomal protein S17 / 40S ribosomal protein S26 / Large ribosomal subunit protein eL37
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit) / Rabbit (rabbit) / Bovine (cattle)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.1 Å
AuthorsPowers KT / Yadav SKN / Bufton JC / Schaffitzel C
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Wellcome Trust210701/Z/18/Z United Kingdom
CitationJournal: Nucleic Acids Res / Year: 2021
Title: Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA.
Authors: Kyle T Powers / Flint Stevenson-Jones / Sathish K N Yadav / Beate Amthor / Joshua C Bufton / Ufuk Borucu / Dakang Shen / Jonas P Becker / Daria Lavysh / Matthias W Hentze / Andreas E Kulozik ...Authors: Kyle T Powers / Flint Stevenson-Jones / Sathish K N Yadav / Beate Amthor / Joshua C Bufton / Ufuk Borucu / Dakang Shen / Jonas P Becker / Daria Lavysh / Matthias W Hentze / Andreas E Kulozik / Gabriele Neu-Yilik / Christiane Schaffitzel /
Abstract: Deciphering translation is of paramount importance for the understanding of many diseases, and antibiotics played a pivotal role in this endeavour. Blasticidin S (BlaS) targets translation by binding ...Deciphering translation is of paramount importance for the understanding of many diseases, and antibiotics played a pivotal role in this endeavour. Blasticidin S (BlaS) targets translation by binding to the peptidyl transferase center of the large ribosomal subunit. Using biochemical, structural and cellular approaches, we show here that BlaS inhibits both translation elongation and termination in Mammalia. Bound to mammalian terminating ribosomes, BlaS distorts the 3'CCA tail of the P-site tRNA to a larger extent than previously reported for bacterial ribosomes, thus delaying both, peptide bond formation and peptidyl-tRNA hydrolysis. While BlaS does not inhibit stop codon recognition by the eukaryotic release factor 1 (eRF1), it interferes with eRF1's accommodation into the peptidyl transferase center and subsequent peptide release. In human cells, BlaS inhibits nonsense-mediated mRNA decay and, at subinhibitory concentrations, modulates translation dynamics at premature termination codons leading to enhanced protein production.
History
DepositionMar 16, 2021-
Header (metadata) releaseJul 7, 2021-
Map releaseJul 7, 2021-
UpdateJan 11, 2023-
Current statusJan 11, 2023Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.014
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.014
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7nwh
  • Surface level: 0.014
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_12632.png

Downloads & links

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Map

FileDownload / File: emd_12632.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.35 Å
Density
Contour LevelBy AUTHOR: 0.014 / Movie #1: 0.014
Minimum - Maximum-0.05001754 - 0.083289854
Average (Standard dev.)0.0010518832 (±0.005058572)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 405.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.351.351.35
M x/y/z300300300
origin x/y/z0.0000.0000.000
length x/y/z405.000405.000405.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS300300300
D min/max/mean-0.0500.0830.001

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Supplemental data

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Mask #1

Fileemd_12632_msk_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_12632_half_map_1.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Half map: #1

Fileemd_12632_half_map_2.map
Projections & Slices
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Sample components

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Entire : Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound b...

EntireName: Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S.
Components
  • Complex: Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S.
    • Protein or peptide: 60S ribosomal protein L8
    • Protein or peptide: uL4
    • Protein or peptide: eL31
    • Protein or peptide: 40S ribosomal protein S3
    • Protein or peptide: S29
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: Ribosomal protein L32
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: eL29
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: eL33
    • Protein or peptide: Ribosomal protein S5
    • Protein or peptide: 40S ribosomal protein S27a
    • Protein or peptide: uL30
    • Protein or peptide: 60S ribosomal protein L34
    • Protein or peptide: 40S ribosomal protein S3a
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: Epididymis tissue sperm binding protein Li 3a
    • Protein or peptide: L7a
    • Protein or peptide: uL29
    • Protein or peptide: S7
    • RNA: mRNAMessenger RNA
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: L9
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: Eukaryotic peptide chain release factor subunit 1
    • Protein or peptide: 60S ribosomal protein L10
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: 40S ribosomal protein S9
    • Protein or peptide: eRF3a
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: L38
    • Protein or peptide: 40S ribosomal protein S10
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: eL39
    • Protein or peptide: 40S ribosomal protein S11
    • Protein or peptide: Ribosomal protein L14
    • Protein or peptide: 60S RIBOSOMAL PROTEIN EL40
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: 60s ribosomal protein l41
    • Protein or peptide: ribosomal protein uS15
    • Protein or peptide: 60S RIBOSOMAL PROTEIN UL13
    • Protein or peptide: eL42
    • Protein or peptide: uS11
    • Protein or peptide: uL22
    • Protein or peptide: ribosomal protein eL43
    • Protein or peptide: 40S ribosomal protein uS19
    • Protein or peptide: eL18
    • Protein or peptide: eL28
    • Protein or peptide: Ribosomal protein S16
    • Protein or peptide: 60S ribosomal protein L19
    • Protein or peptide: 60S acidic ribosomal protein P0
    • Protein or peptide: 40S ribosomal protein S17
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: uL12
    • Protein or peptide: 40S ribosomal protein uS13
    • Protein or peptide: eL21
    • Protein or peptide: eS19
    • Protein or peptide: L22
    • Protein or peptide: 40S ribosomal protein S20
    • Protein or peptide: eL14
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: 60S ribosomal protein L24-like proteinRibosome
    • RNA: 28S Ribosomal RNA
    • Protein or peptide: Ribosomal protein S15aRibosome
    • Protein or peptide: uL23
    • RNA: 5S Ribosomal RNA
    • Protein or peptide: 40S ribosomal protein uS12
    • Protein or peptide: Ribosomal protein L26
    • RNA: 5.8S Ribosomal RNA
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 60S ribosomal protein L27
    • RNA: 18S Ribosomal RNA
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: 60S ribosomal protein L27a
    • Protein or peptide: 40S_SA_C domain-containing protein
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: uL3
    • Protein or peptide: eL30
    • Protein or peptide: 40S ribosomal protein S2
    • Protein or peptide: 40S ribosomal protein S28
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER
  • Ligand: BLASTICIDIN S

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Supramolecule #1: Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound b...

SupramoleculeName: Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S.
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#84
Details: Rabbit reticulocyte lysate derived (in vitro transcription-FLAG affinity purified) ribosomal complex.
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 3.8 MDa

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Macromolecule #1: 60S ribosomal protein L8

MacromoleculeName: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 27.129809 KDa
SequenceString: MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV ...String:
MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGNHQH IGKPSTIRRD APAGRKVGLI AA RRTGRLR GT

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Macromolecule #2: uL4

MacromoleculeName: uL4 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 42.825609 KDa
SequenceString: MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV ...String:
MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC VIYNEDNGIV KAFRNIPGIT LLNVTKLNIL KL APGGHVG RFCIWTESAF RKLDDLYGTW RKAASLKSNY NLPMHKMLNT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVERAAA ALAAKSDPKE APAKKKPVVG KK

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Macromolecule #3: eL31

MacromoleculeName: eL31 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.76689 KDa
SequenceString:
MNEVVTREYT INIHKRIHGV GFKKRAPRAL KEIRKFAMKE MGTPDVRIDT RLNKAVWAKG IRNVPYRIRV RLSRKRNEDE DSPNKLYTL VTYVPVTTFK NLQTVNVDE

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Macromolecule #4: 40S ribosomal protein S3

MacromoleculeName: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 31.146607 KDa
SequenceString: MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG ...String:
MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPSGKIGP KK PLPDHVS IVEPKDEILP TTPISEQKGG KPEPPAMPQP VPTA

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Macromolecule #5: S29

MacromoleculeName: S29 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 6.690821 KDa
SequenceString:
MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

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Macromolecule #6: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 34.350633 KDa
SequenceString: GFVKVVKNKA YFKRYQVKFR RRREGKTDYY ARKRLVIQDK NKYNTPKYRM IVRVTNRDII CQIAYARIEG DMIVCAAYAH ELPKYGVKV GLTNYAAAYC TGLLLARRLL NRFGMDKIYE GQVEVTGDEY NVESIDGQPG AFTCYLDAGL ARTTTGNKVF G ALKGAVDG ...String:
GFVKVVKNKA YFKRYQVKFR RRREGKTDYY ARKRLVIQDK NKYNTPKYRM IVRVTNRDII CQIAYARIEG DMIVCAAYAH ELPKYGVKV GLTNYAAAYC TGLLLARRLL NRFGMDKIYE GQVEVTGDEY NVESIDGQPG AFTCYLDAGL ARTTTGNKVF G ALKGAVDG GLSIPHSTKR FPGYDSESKE FNAEVHRKHI MGQNVADYMR YLMEEDEDAY KKQFSQYIKN NVTPDMMEEM YK KAHAAIR ENPVYEKKPK REVKKKRWNR PKMSLAQKKD RVAQKKASFL RAQERAAES

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Macromolecule #7: Ribosomal protein L32

MacromoleculeName: Ribosomal protein L32 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.153217 KDa
SequenceString:
MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL

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Macromolecule #8: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.654869 KDa
SequenceString: MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ...String:
MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

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Macromolecule #9: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.498884 KDa
SequenceString:
MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

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Macromolecule #10: eL29

MacromoleculeName: eL29 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.931625 KDa
SequenceString: MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE ...String:
MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE TKPKAQAQAK PKAQAQGKPK AQAQGKPKAQ AQAKPKAQAQ AKPKAQAQTK PKAQATPA

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Macromolecule #11: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 33.028336 KDa
SequenceString: MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL ...String:
MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKKLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

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Macromolecule #12: eL33

MacromoleculeName: eL33 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.580809 KDa
SequenceString:
MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

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Macromolecule #13: Ribosomal protein S5

MacromoleculeName: Ribosomal protein S5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 22.913453 KDa
SequenceString: MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT ...String:
MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

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Macromolecule #14: 40S ribosomal protein S27a

MacromoleculeName: 40S ribosomal protein S27a / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 7.98644 KDa
SequenceString:
KSYTTPKKNK HKRKKVKLAV LKYYKVDENG KISRLRRECP SDECGAGVFM ASHFDRHYCG KCCLTYCF

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Macromolecule #15: uL30

MacromoleculeName: uL30 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.51425 KDa
SequenceString: LFANNIREKK KVPAVPETLK KKRRNFAELK IKRLRKKFAQ KMLRKARRKL IYEKAKHYHK EYRQMYRTEI RMARMARKAG NFYVPAEPK LAFVIRIRGI NGVSPKVRKV LQLLRLRQIF NGTFVKLNKA SINMLRIVEP YIAWGYPNLK SVNELIYKRG Y GKINKKRI ...String:
LFANNIREKK KVPAVPETLK KKRRNFAELK IKRLRKKFAQ KMLRKARRKL IYEKAKHYHK EYRQMYRTEI RMARMARKAG NFYVPAEPK LAFVIRIRGI NGVSPKVRKV LQLLRLRQIF NGTFVKLNKA SINMLRIVEP YIAWGYPNLK SVNELIYKRG Y GKINKKRI ALTDNTLIAR SLGKYNIICM EDLIHEIYTV GKHFKEANNF LWPFKLSSPR GGMKKKTTHF VEGGDAGNRE DQ INRLIRR MN

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Macromolecule #16: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.210088 KDa
SequenceString:
LPGRCLEAAT MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYG GSMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKA

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Macromolecule #17: 40S ribosomal protein S3a

MacromoleculeName: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.94201 KDa
SequenceString: MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM ...String:
MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGGSS GK AAGDETG AKVERADGYE PPVQESV

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Macromolecule #18: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 30.201631 KDa
SequenceString: MLFRGASEAV GSLKMKLNIS FPATGCQKLI EVDDERKLRT FYEKRMATEV AADALGEEWK GYVVRISGGN DKQGFPMKQG VLTHGRVRL LLSKGHSCYR PRRTGERKRK SVRGCIVDAN LSVLNLVIVK KGEKDIPGLT DTTVPRRLGP KRASRIRKLF N LSKEDDVR ...String:
MLFRGASEAV GSLKMKLNIS FPATGCQKLI EVDDERKLRT FYEKRMATEV AADALGEEWK GYVVRISGGN DKQGFPMKQG VLTHGRVRL LLSKGHSCYR PRRTGERKRK SVRGCIVDAN LSVLNLVIVK KGEKDIPGLT DTTVPRRLGP KRASRIRKLF N LSKEDDVR QYVVRKPLNK EGKKPRTKAP KIQRLVTPRV LQHKRRRIAL KKQRTKKNKE EAAEYAKLLA KRMKEAKEKR QE QIAKRRR LSSLRASTSK SESSQK

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Macromolecule #19: Epididymis tissue sperm binding protein Li 3a

MacromoleculeName: Epididymis tissue sperm binding protein Li 3a / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 34.800305 KDa
SequenceString: MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC ...String:
MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTI

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Macromolecule #20: L7a

MacromoleculeName: L7a / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 27.61175 KDa
SequenceString: MKVVNPLFEK RPKNFGIGQD IQPKRDLTRF VKWPRYIRLQ RQRAILYKRL KVPPAINQFT QVLDRQTATQ LLKLAHKYRP ETKQEKKQR LLARAEKKAA GKGDVPTKRP PVLRAGVNTV TTLVENKKAQ LVVIAHDVDP IELVVFLPAL CRKMGVPYCI L KGKARLGR ...String:
MKVVNPLFEK RPKNFGIGQD IQPKRDLTRF VKWPRYIRLQ RQRAILYKRL KVPPAINQFT QVLDRQTATQ LLKLAHKYRP ETKQEKKQR LLARAEKKAA GKGDVPTKRP PVLRAGVNTV TTLVENKKAQ LVVIAHDVDP IELVVFLPAL CRKMGVPYCI L KGKARLGR LVHRKTCTTV AFTQVNSEDK GALAKLVEAI RTNYNDRYDE IRRHWGGNVL GPKSVARIAK LEKAKAKELA TK LG

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Macromolecule #21: uL29

MacromoleculeName: uL29 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.566599 KDa
SequenceString:
MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ESLKTKKQQR KERLYPLRKY AVKA

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Macromolecule #22: S7

MacromoleculeName: S7 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 21.847582 KDa
SequenceString:
MSAKIVKPNG EKPDEFESGI SQALLELEMN SDLKAQLREL NITAAKEIEV GGGRKAIIIF VPVPQLKSFQ KIQVRLVREL EKKFSGKHV VFIAQRRILP KPTRKSRTKN KQKRPRSRTL TAVHDAILED LVFPSEIVGK RIRVKLDGSR LIKVHLDKAQ Q NNVEHKVE TFSGVYKKLT GKDVNFEFPE FQL

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Macromolecule #24: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 9.480186 KDa
SequenceString:
MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

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Macromolecule #25: L9

MacromoleculeName: L9 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.627215 KDa
SequenceString:
MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QA

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Macromolecule #26: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.317796 KDa
SequenceString:
AAATALRYPM AVGLNKGHKV TKNVSKPRHS RRRGRLTKHT KFVRDMIREV CGFAPYERRA MELLKVSKDK RALKFIKKRV GTHIRAKRK REELSSVLAA MRKAAAKD

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Macromolecule #27: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.263387 KDa
SequenceString: MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ ...String:
MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

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Macromolecule #28: Eukaryotic peptide chain release factor subunit 1

MacromoleculeName: Eukaryotic peptide chain release factor subunit 1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 49.092742 KDa
SequenceString: MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK MLADEFGTAS NIKSRVNRLS VLGAITSVQQ RLKLYNKVP PNGLVVYCGT IVTEEGKEKK VNIDFEPFKP INTSLYLCDN KFHTEALTAL LSDDSKFGFI VIDGSGALFG T LQGNTREV ...String:
MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK MLADEFGTAS NIKSRVNRLS VLGAITSVQQ RLKLYNKVP PNGLVVYCGT IVTEEGKEKK VNIDFEPFKP INTSLYLCDN KFHTEALTAL LSDDSKFGFI VIDGSGALFG T LQGNTREV LHKFTVDLPK KHGRGGQSAL RFARLRMEKR HNYVRKVAET AVQLFISGDK VNVAGLVLAG SADFKTELSQ SD MFDQRLQ SKVLKLVDIS YGGENGFNQA IELSTEVLSN VKFIQEKKLI GRYFDEISQD TGKYCFGVED TLKALEMGAV EIL IVYENL DIMRYVLHCQ GTEEEKILYL TPEQEKDKSH FTDKETGQEH ELIESMPLLE WFANNYKKFG ATLEIVTDKS QEGS QFVKG FGGIGGILRY RVDFQGMEYQ GGDDEFFDLD DY

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Macromolecule #29: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.643057 KDa
SequenceString: MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS ...String:
MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

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Macromolecule #30: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 11.111032 KDa
SequenceString:
MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

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Macromolecule #31: 40S ribosomal protein S9

MacromoleculeName: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 22.65559 KDa
SequenceString: MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG ...String:
MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG EDEEED

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Macromolecule #32: eRF3a

MacromoleculeName: eRF3a / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 48.0565 KDa
SequenceString: APKKEHVNVV FIGHVDAGKS TIGGQIMYLT GMVDKRTLEK YEREAKEKNR ETWYLSWALD TNQEERDKGK TVEVGRAYFE TEKKHFTIL DAPGHKSFVP NMIGGASQAD LAVLVISARK GEFETGFEKG GQTREHAMLA KTAGVKHLIV LINKMDDPTV N WSNERYEE ...String:
APKKEHVNVV FIGHVDAGKS TIGGQIMYLT GMVDKRTLEK YEREAKEKNR ETWYLSWALD TNQEERDKGK TVEVGRAYFE TEKKHFTIL DAPGHKSFVP NMIGGASQAD LAVLVISARK GEFETGFEKG GQTREHAMLA KTAGVKHLIV LINKMDDPTV N WSNERYEE CKEKLVPFLK KVGFNPKKDI HFMPCSGLTG ANLKEQSDFC PWYIGLPFIP YLDNLPNFNR SVDGPIRLPI VD KYKDMGT VVLGKLESGS ICKGQQLVMM PNKHNVEVLG ILSDDVETDT VAPGENLKIR LKGIEEEEIL PGFILCDPNN LCH SGRTFD AQIVIIEHKS IICPGYNAVL HIHTCIEEVE ITALICLVDK KSGEKSKTRP RFVKQDQVCI ARLRTAGTIC LETF KDFPQ MGRFTLRDEG KTIAIGKVLK LVP

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Macromolecule #33: 60S ribosomal protein L11

MacromoleculeName: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 20.086191 KDa
SequenceString:
QDQGEKENPM RELRIRKLCL NICVGESGDR LTRAAKVLEQ LTGQTPVFSK ARYTVRSFGI RRNEKIAVHC TVRGAKAEEI LEKGLKVRE YELRKNNFSD TGNFGFGIQE HIDLGIKYDP SIGIYGLDFY VVLGRPGFSI ADKKRRTGCI GAKHRISKEE A MRWFQQKY DGIILPGK

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Macromolecule #34: L38

MacromoleculeName: L38 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 8.238948 KDa
SequenceString:
MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

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Macromolecule #35: 40S ribosomal protein S10

MacromoleculeName: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.422133 KDa
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPSGADKK AEAGAGSATE FQ

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Macromolecule #36: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.331723 KDa
SequenceString: MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ...String:
MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

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Macromolecule #37: eL39

MacromoleculeName: eL39 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 6.455775 KDa
SequenceString:
MSSHKTFRIK RFLAKKQKQN RPIPQWIWMK TGNKIRYNSK RRHWRRTKLG L

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Macromolecule #38: 40S ribosomal protein S11

MacromoleculeName: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.513824 KDa
SequenceString:
MADIQTERAY QKQPTIFQNK KRVLQGETSK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

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Macromolecule #39: Ribosomal protein L14

MacromoleculeName: Ribosomal protein L14 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.870549 KDa
SequenceString: MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG ...String:
MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG KKAAAQKAPA QKAPAQKAAG QKAAQPPKAQ KGQKPPAQKA PAPKASGKKA

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Macromolecule #40: 60S RIBOSOMAL PROTEIN EL40

MacromoleculeName: 60S RIBOSOMAL PROTEIN EL40 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.69531 KDa
SequenceString:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGLTLSDYN IQKESTLHLV LRLHGGIIEP SLRQLAQKY NCDKMICRKC YARLHPRAVN CRKKKCGHTN NLRPKKKVK

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Macromolecule #41: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.70907 KDa
SequenceString:
GVMDVNTALQ EVLKTALIHD GLARGIREAA KALDKRQAHL CVLASNCDEP MYVKLVEALC AEHQINLIKV DDNKKLGEWV GLCKIDREG KPRKVVGCSC VVVKDYGKES QAKDVIEEYF KCKK

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Macromolecule #42: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.207285 KDa
SequenceString: MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG ...String:
MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

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Macromolecule #43: 60s ribosomal protein l41

MacromoleculeName: 60s ribosomal protein l41 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 3.473451 KDa
SequenceString:
MRAKWRKKRM RRLKRKRRKM RQRSK

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Macromolecule #44: ribosomal protein uS15

MacromoleculeName: ribosomal protein uS15 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.188309 KDa
SequenceString:
MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL V

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Macromolecule #45: 60S RIBOSOMAL PROTEIN UL13

MacromoleculeName: 60S RIBOSOMAL PROTEIN UL13 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 23.533299 KDa
SequenceString: MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY ...String:
MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK ISKFTDVLKT HGLLV

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Macromolecule #46: eL42

MacromoleculeName: eL42 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.170214 KDa
SequenceString:
MVAVSHGSEE VHSGTAYKLP LLL(UNK)PLSFRA ASAPANMVNV PKTRRTFCKK CGKHQPHKVT QYKKGKDSLY AQGKRR YDR KQSGYGGQTK PIFRKKAKTT KKIVLRLECV EPNCRSKRML AIKRCKHFEL GGDKKRKGQV IQF

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Macromolecule #47: uS11

MacromoleculeName: uS11 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.916512 KDa
SequenceString:
RRRAAMAPRK GKEKKEEQVI SLGPQVAEGE NVFGVCHIFA SFNDTFVHVT DLSGKETICR VTGGMKVKAD RDESSPYAAM LAAQDVAQR CKELGITALH IKLRATGGNR TKTPGPGAQS ALRALARSGM KIGRIEDVTP IPSDSTRRKG GRRGRRL

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Macromolecule #48: uL22

MacromoleculeName: uL22 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.056182 KDa
SequenceString: MRKALPVSGQ RSPAKMVRYS LDPENPTKSC KSRGSNLRVH FKNTRETAQA IKGMHIRKAT KYLKDVTLKK QCVPFRRYNG GVGRCAQAK QWGWTQGRWP KKSAEFLLHM LKNAESNAEL KGLDVDSLVI EHIQVNKAPK MRRRTYRAHG RINPYMSSPC H IEMILTEK ...String:
MRKALPVSGQ RSPAKMVRYS LDPENPTKSC KSRGSNLRVH FKNTRETAQA IKGMHIRKAT KYLKDVTLKK QCVPFRRYNG GVGRCAQAK QWGWTQGRWP KKSAEFLLHM LKNAESNAEL KGLDVDSLVI EHIQVNKAPK MRRRTYRAHG RINPYMSSPC H IEMILTEK EQIVPKPEEE VAQKKKISQK KLKKQKLMAR E

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Macromolecule #49: ribosomal protein eL43

MacromoleculeName: ribosomal protein eL43 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.010391 KDa
SequenceString:
MRIASLPLRA LGLGSGDMAK RTKKVGIVGK YGTRYGASLR KMVKKIEISQ HAKYTCSFCG KTKMKRRAVG IWHCGSCMKT VAGGAWTYN TTSAVTVKSA IRRLKELKDQ

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Macromolecule #50: 40S ribosomal protein uS19

MacromoleculeName: 40S ribosomal protein uS19 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.049182 KDa
SequenceString:
MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

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Macromolecule #51: eL18

MacromoleculeName: eL18 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.699688 KDa
SequenceString:
MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTVTDD VRVQEVPKLK VCALRVTSRA RSRILKAGGK ILTFDQLALD SPKGRGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

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Macromolecule #52: eL28

MacromoleculeName: eL28 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.783614 KDa
SequenceString:
MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYHPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

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Macromolecule #53: Ribosomal protein S16

MacromoleculeName: Ribosomal protein S16 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.716932 KDa
SequenceString:
MRGVELVLVL GAMPSKGPLQ SVQVFGRKKT ATAVAHCKRG NGLIKVNGRP LEMIEPRTLQ YKLLEPVLLL GKERFAGVDI RVRVKGGGH VAQIYAIRQS ISKALVAYYQ KYVDEASKKE IKDILIQYDR TLLVADPRRC ESKKFGGPGA RARYQKSYR

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Macromolecule #54: 60S ribosomal protein L19

MacromoleculeName: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.535281 KDa
SequenceString: MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ...String:
MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

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Macromolecule #55: 60S acidic ribosomal protein P0

MacromoleculeName: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 34.380504 KDa
SequenceString: MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ...String:
MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ASEATLLNML NISPFSFGLI IQQVFDNGSI YNPEVLDITE DTLHSRFLEG VRNVASVCLQ IGYPTVASVP HS IINGYKR VLALSVETEY TFPLAEKVKA FLADPSAFVA AAPVAAASTA APAAAAAAPA KVEAKEESEE SDEDMGFGLF D

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Macromolecule #56: 40S ribosomal protein S17

MacromoleculeName: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.782648 KDa
SequenceString:
MKSKKELRVQ KGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERE RRDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

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Macromolecule #57: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 20.827561 KDa
SequenceString:
MKASGTLREY KVVGRCLPTP KCRTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

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Macromolecule #58: uL12

MacromoleculeName: uL12 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.426787 KDa
SequenceString: MAFRYSARRR PRCNSLRSSR IRVHPTPGAS TMPPKFDPNE IKVVYLRCTG GEVGATSALA PKIGPLGLSP KKVGDDIAKA TGDWKGLRI TVKLTIQNRQ AQIEVVPSAS ALIIKALKEP PRDRKKQKNI KHSGNITFDE IVNIARQMRH RSLARELSGT I KEILGTAQ ...String:
MAFRYSARRR PRCNSLRSSR IRVHPTPGAS TMPPKFDPNE IKVVYLRCTG GEVGATSALA PKIGPLGLSP KKVGDDIAKA TGDWKGLRI TVKLTIQNRQ AQIEVVPSAS ALIIKALKEP PRDRKKQKNI KHSGNITFDE IVNIARQMRH RSLARELSGT I KEILGTAQ SVGCNVDGRH PHDIIDDINS GAVECPAS

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Macromolecule #59: 40S ribosomal protein uS13

MacromoleculeName: 40S ribosomal protein uS13 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.759777 KDa
SequenceString:
MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

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Macromolecule #60: eL21

MacromoleculeName: eL21 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.609988 KDa
SequenceString:
MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

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Macromolecule #61: eS19

MacromoleculeName: eS19 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.10664 KDa
SequenceString:
MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AAKKKH

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Macromolecule #62: L22

MacromoleculeName: L22 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.798989 KDa
SequenceString:
MAPVKKLVAK GGKKKKQLLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VSLERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANTKES YELRYFQINQ DEEEEEDED

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Macromolecule #63: 40S ribosomal protein S20

MacromoleculeName: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 13.268555 KDa
SequenceString:
AFKDTGKAPV EQEVAIHRIR ITLTSRNVKS LEKVCADLIR GAKEKNLKVK GPVRMPTKTL RITTRKTPCG EGSKTWDRFQ MRIHKRLID LHSPSEIVKQ ITSISIEPGV EVEVTIADA

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Macromolecule #64: eL14

MacromoleculeName: eL14 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.10362 KDa
SequenceString:
MSGAKFRISL GLPVGAVINC ADNTGAKNLY IISVKGIKGR LNRLPAAGVG DMVMATVKKG KPELRKKVHP AVVIRQRKSY RRKDGVFLY FEDNAGVIVN NKGEMKGSAI TGPVAKECAD LWPRIASNAG SIA

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Macromolecule #65: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 9.124389 KDa
SequenceString:
MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

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Macromolecule #66: 60S ribosomal protein L24-like protein

MacromoleculeName: 60S ribosomal protein L24-like protein / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.538256 KDa
SequenceString:
MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKEKR

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Macromolecule #68: Ribosomal protein S15a

MacromoleculeName: Ribosomal protein S15a / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.778584 KDa
SequenceString:
MHEASLAATM VRMNVLADAL KSINNAEKRG KRQVLIRPCS KVIVRFLTVM MKHGYIGEFE IIDDHRAGKI VVNLTGRLNK CGVISPRFD VQLKDLEKWQ NNLLPSRQFG FIVLTTSAGI MDHEEARRKH TGGKILGFFF

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Macromolecule #69: uL23

MacromoleculeName: uL23 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.768246 KDa
SequenceString:
MAPKAKKEAP APPKVEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

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Macromolecule #71: 40S ribosomal protein uS12

MacromoleculeName: 40S ribosomal protein uS12 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.757587 KDa
SequenceString:
MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPR

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Macromolecule #72: Ribosomal protein L26

MacromoleculeName: Ribosomal protein L26 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.891787 KDa
SequenceString:
MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGK

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Macromolecule #74: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.007125 KDa
SequenceString:
MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KEVSWRWITA EGVKILQ

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Macromolecule #75: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.835831 KDa
SequenceString:
MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

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Macromolecule #77: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.450798 KDa
SequenceString:
KDDKKKKDAG KSAKKDKDPV NKSGGKAKKK KWSKGKVRDK LNNLVLFDKA TYDKLCKEVP NYKLITPAVV SERLKIRGSL ARAALQELL SKGLIKLVSK HRAQVIYTRN TKGGDAPAAG EDA

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Macromolecule #78: 60S ribosomal protein L27a

MacromoleculeName: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.489363 KDa
SequenceString:
PSRLRKTRKL RGHVSHGHGR IGKHRKHPGG RGNAGGMHHH RINFDKYHPG YFGKVGMRHY HLKRNQSFCP TVNLDKLWTL VSEQTRVNA AKNKTGAAPI IDVVRSGYYK VLGKGKLPKQ PVIVKAKFFS RRAEEKIKGV GGACVLVA

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Macromolecule #79: 40S_SA_C domain-containing protein

MacromoleculeName: 40S_SA_C domain-containing protein / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 32.958016 KDa
SequenceString: MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQTAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA ...String:
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQTAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGTTTEWS

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Macromolecule #80: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.147561 KDa
SequenceString:
MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAVSVAL STADMVGA

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Macromolecule #81: uL3

MacromoleculeName: uL3 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 45.976781 KDa
SequenceString: SHRKFSAPRH GSLGFLPRKR SSRHRGKVKS FPKDDPSKPV HLTAFLGYKA GMTHIVREVD RPGSKVNKKE VVEAVTIVET PPMVVVGIV GYVETPRGLR TFKTVFAEHI SDECKRRFYK NWHKSKKKAF TKYCKKWQDD AGKRQLDKDF SSMKKYCQVI R VLAHTQMR ...String:
SHRKFSAPRH GSLGFLPRKR SSRHRGKVKS FPKDDPSKPV HLTAFLGYKA GMTHIVREVD RPGSKVNKKE VVEAVTIVET PPMVVVGIV GYVETPRGLR TFKTVFAEHI SDECKRRFYK NWHKSKKKAF TKYCKKWQDD AGKRQLDKDF SSMKKYCQVI R VLAHTQMR LLPLRQKKAH LMEIQVNGGT VAEKLDWARE RLEQQVPVSQ VFGQDEMIDV IGVTKGKGYK GVTSRWHTKK LP RKTHRGL RKVACIGAWH PARVAFSVAR AGQKGYHHRT EINKKIYKIG QGYLIKDGKL IKNNASTDYD LSDKSINPLG GFV HYGEVT NDFVMLKGCV VGTKKRVLTL RKSLLVQTKR RALEKIDLKF IDTTSKFGHG RFQTVEEKKA FMGPLKKDRI AKEE GA

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Macromolecule #82: eL30

MacromoleculeName: eL30 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.807065 KDa
SequenceString:
MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQSLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLSIIDP GDSDIIRSMP EQTGEK

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Macromolecule #83: 40S ribosomal protein S2

MacromoleculeName: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Bovine (cattle)
Molecular weightTheoretical: 27.995727 KDa
SequenceString: MADDAGAAGG PGGLGARGGK AEDKEWLPVT KLGRLVKDMK IKSLEEIYLF SLPIKESEII DFFLGASLKD EVLKIMPVQK QTRAGQRTR FKAFVAIGDY NGHVGLGVKC SKEVATAIRG AIILAKLSIV PVRRGYWGNK IGKPHTVPCK VTGRCGSVLV R LIPAPRGT ...String:
MADDAGAAGG PGGLGARGGK AEDKEWLPVT KLGRLVKDMK IKSLEEIYLF SLPIKESEII DFFLGASLKD EVLKIMPVQK QTRAGQRTR FKAFVAIGDY NGHVGLGVKC SKEVATAIRG AIILAKLSIV PVRRGYWGNK IGKPHTVPCK VTGRCGSVLV R LIPAPRGT GIVSAPVPKK LLMMAGIDDC YTSARGCTAT LGNFAKATFD AISKTYSYLT PDLWKETVFT KSPYQEFTDH LV KTHTRVS VQRTQAPAVA TT

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Macromolecule #84: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 7.836005 KDa
SequenceString:
MDTSHVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

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Macromolecule #23: mRNA

MacromoleculeName: mRNA / type: rna / ID: 23 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 4.753857 KDa
SequenceString:
UCAAAGUUUG AGCUC

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Macromolecule #67: 28S Ribosomal RNA

MacromoleculeName: 28S Ribosomal RNA / type: rna / ID: 67 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 1.19969525 MDa
SequenceString: CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ...String:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGACACAC AGGGGGCCCA AGUCCUUCUG AUCGAGGCCC AGCCCGUGGA CGGUGUGAGG CC GGUAGCG GCCCCCGGCG CGCCGGGCCC GGGUCUUCCC GGAGUCGGGU UGCUUGGGAA UGCAGCCCAA AGCGGGUGGU AAA CUCCAU CUAAGGCUAA AUACCGGCAC GAGACCGAUA GUCAACAAGU ACCGUAAGGG AAAGUUGAAA AGAACUUUGA AGAG AGAGU UCAAGAGGGC GUGAAACCGU UAAGAGGUAA ACGGGUGGGG UCCGCGCAGU CCGCCCGGAG GAUUCAACCC GGCGG CGCG GUCCGGCCGU GCCGGCGGAC AGGCGGAUCU ACCCCGCUCC CACACACACA CAGUCGGCGG GGGACCGCCC CCCGGC CGG CGACCGGCCG CCGCCGGGCG CAUUUCCACC GCGGCGGUGC GCCGCGACCG GCUCCGGGAC GGCUGGGAAG GCCCCGG CG GGGAAGGUGG CCCGGGGGAC ACACACACAC CCCGGGUGUU ACAGCCCCGC CCCGGCAGCA GCAGCUCGCC GAAUCCCG G GGCCGAGGGA GCGAGACCCG UGCGCCGCGC UCUCCCCCCU CGGGGGGGCC ACACACACAC AGGGCCACCC CUCCCACGG CGCGACCGCU ACACACACAC AGGGGCGGAC UGUCACCAGU GCGCCCCGGG CGGGUCGCGC CGUCGGGCCC GGGACACACA CACAGGGCC ACACACGCGU CCCCCACACA CACACAGCGA GCGCACGGGG UCGGCGGCGA UGUCGGCUAC CCACCCGACC C GUCUUGAA ACACGGACCA AGGAGUCUAA CACGUGCGCG AGUCGGGGGC UACAACGAAA GCCGCCGUGG CGCAAUGAAG GU GAAGGCC GGCGGCGCGC CGCCGGCCAC GAGGUGGGAU CCCGAGGCCU UCCAGUCCGC CGAGGGCGCA CCACCGGCCC GUC UCGCCC GCCGCGCCGG GGAGGUGGAG CACGAGCGCA CGUGUUAGGA CCCGAAAGAU GGUGAACUAU GCCUGGGCAG GGCG AAGCC AGAGGAAACU CUGGUGGAGG UCCGUAGCGG UCCUGACGUG CGAAUCGGUC GUCCGACCUG GGUAUAGGGG CGAAA GACU AAUCGAACCA UCUAGUAGCU GGUUCCCUCC AAAGUUUCCC UCAGGAUAGC UGGCGCUCUC ACACACACAC ACGCAG UUU UAUCCGGUAA AGCGAAUGAU UAGAGGUCUU GGGGCCGAAA CGAUCUCAAC CUAUUCUCAA ACUUUAAAUG GGUAAGA AG CCCGGCUCGC UGGUGUGGAG CCGGGCGUGG AAUGCGAGUG CCUAGUGGGC CACUUUUGGU AAGCAGAACU GGCGCUGC G GGAUGAACCG AACGCCGGGU UAAGGCGCCC GAUGCCGACG CUCAUCAGAC CCCAGAAAAG GUGUUGGUUG AUAUAGACA GCAGGAUGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACCUGCCG AAUCAACUAG CCCUGAAAAU GGAUGGCGC UGGAGCGUCG GGCCCAUACC CGGCCGUCGC UGGCAGUCGG CAGAGCGAGA AAGAGAGUGG ACACACACAC A CGCGGACG CUACGCCGCG ACGAGUAGGA GGGCCGCUGC GGUGAGCCUU GAAGCCUAGG GCGCGGGCCC GGGUGGAGCC GC CGCAGGU GCAGAUCUUG GUGGUAGUAG CAAAUAUUCA AACGAGAACU UUGAAGGCCG AAGUGGAGAA GGGUUCCAUG UGA ACAGCA GUUGAACAUG GGUCAGUCGG UCCUGAGAGA UGGGCGAGCG CCGUUCCGAA GGGACGGGCG AUGGCCUCCG UUGC CCUCA GCCGAUCGAA AGGGAGUCGG GUUCAGAUCC CCGAAUCCGG AGUGGCGGAG AUGGGCGCCG CGAGGCGUCC AGUGC GGUA ACGCGACCGA UCCCGGAGAA GCCGGCGGGA GCCCCGGGGA GAGUUCUCUU UUCUUUGUGA AGGGCAGGGC GCCCUG GAA UGGGUUCGCC CCGAGAGAGG GGCCCGUGCC UUGGAAAGCG UCGCGGUUCC GGCGGCGUCC GGUGAGCUCU CGCUGGC CC UUGAAAAUCC GGGGGAGAGG GUGUAAAUCU CGCGCCGGGC CGUACCCAUA UCCGCAGCAG GUCUCCAAGG UGAACAGC C UCUGGCAUGU UGGAACAAUG UAGGUAAGGG AAGUCGGCAA GCCGGAUCCG UAACUUCGGG AUAAGGAUUG GCUCUAAGG GCUGACACAC ACACAGCAGC CGACUUAGAA CUGGUGCGGA CCAGGGGAAU CCGACUGUUU AAUUAAAACA AAGCAUCGCG AAGGCCCGC GGCGGGUGUU GACGCGAUGU GAUUUCUGCC CAGUGCUCUG AAUGUCAAAG UGAAGAAAUU CAAUGAAGCG C GGGUAAAC GGCGGGAGUA ACUAUGACUC UCUUAAGGUA GCCAAAUGCC UCGUCAUCUA AUUAGUGACG CGCAUGAAUG GA UGAACGA GAUUCCCACU GUCCCUACCU ACUAUCCAGC GAAACCACAG CCAAGGGAAC GGGCUUGGCG GAAUCAGCGG GGA AAGAAG ACCCUGUUGA GCUUGACUCU AGUCUGGCAC GGUGAAGAGA CACACACACA CAGUUUUUUC ACUGACCCGG UGAG GCGGG GGGGCGAGCC ACACAGGGCU CUCGCUUCUG GCGCCAAGCG CCCGGCCGGC CGGGCGCGAC CCGCUCCGGG GACAG UGCC AGGUGGGGAG UUUGACUGGG GCGGUACACC UGUCAAACGG UAACGCAGGU GUCCUAAGGC GAGCUCAGGG AGGACA GAA ACCUCCCGUG GAGCAGAAGG GCAAAAGCUC GCUUGAUCUU GAUUUUCAGU ACGAAUACAG ACCGUGAAAG CGGGGCC UC ACGAUCCUUC UGACCUUUUG GGUUUUAAGC AGGAGGUGUC AGAAAAGUUA CCACAGGGAU AACUGGCUUG UGGCGGCC A AGCGUUCAUA GCGACGUCGC UUUUUGAUCC UUCGAUGUCG GCUCUUCCUA UCAUUGUGAA GCAGAAUUCA CCAAGCGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGUCGUG AGACAGGUUA GUUUUACCCU ACUGAUGAUG UGUUGUUGC CAUGGUAAUC CUGCUCAGUA CGAGAGGAAC CGCAGGUUCA GACAUUUGGU GUAUGUGCUU GGCUGAGGAG C CAAUGGGG CGAAGCUACC AUCUGUGGGA UUAUGACUGA ACGCCUCUAA GUCAGAAUCC CGCCCAGGCG GAACGAUAAC AC GCGCCGA CGAGCCUCGG UUGGCCUCGG AUAGCCGGUC CCCCGCACAC ACACACACGG GGUCCGGUGC GGAGAGCCCU UCG UCCCGG GAAACGGGGA CCGGCCGGAA AGGGGGCCGC CCUCUCGCCC GUCACGCACC GCACGUUCGU GGGGAACCUG GCGC UAAAC CAUUCGUAGA CGACCUGCUU CUGGGUCGGG GUUUCGUACG UAGCAGAGCA GCUACACAGC UGCGAUCUAU UGAAA GUCA GCCCUCGACA CAAGGGUUUG U

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Macromolecule #70: 5S Ribosomal RNA

MacromoleculeName: 5S Ribosomal RNA / type: rna / ID: 70 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 38.691914 KDa
SequenceString:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

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Macromolecule #73: 5.8S Ribosomal RNA

MacromoleculeName: 5.8S Ribosomal RNA / type: rna / ID: 73 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 48.545672 KDa
SequenceString:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGU UGAUCAUCG ACACUUCGAA CGCACUUGCG GCCCCGGGUU CCUCCCGGGG CUACGCCUGU CUGAGCGUCG CU

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Macromolecule #76: 18S Ribosomal RNA

MacromoleculeName: 18S Ribosomal RNA / type: rna / ID: 76 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 573.842125 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GACACACACA UACUUGGAUA ACUGUGGUAA U UCUAGAGC ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GACACACACA UACUUGGAUA ACUGUGGUAA U UCUAGAGC UAAUACAUGC CGACGGGCGC UGACCCCCUU CGCGGGGGGG AUGCGUGCAU UUAUCAGACA CACACACAUG GU GACUCUA GAUAACCUCG GGCCGAUCGC ACGCCCACAC AGGCGGCGAC GACCCAUUCG AACGUCUGCC CUAUCAACUU UCG AUGGUA GUCGCCGUGC CUACCAUGGU GACCACGGGU GACGGGGAAU CAGGGUUCGA UUCCGGAGAG GGAGCCUGAG AAAC GGCUA CCACAUCCAA GGAAGGCAGC AGGCGCGCAA AUUACCCACU CCCGACCCGG GGAGGUAGUG ACGAAAAAUA ACAAU ACAG GACUCUUUCG AGGCCCUGUA AUUGGAAUGA GUCCACUUUA AAUCCUUUAA CGAGGAUCCA UUGGAGGGCA AGUCUG GUG CCAGCAGCCG CGGUAAUUCC AGCUCCAAUA GCGUAUAUUA AAGUUGCUGC AGUUAAAAAG CUCGUAGUUG GAUCUUG GG AGCGGACACA CACACACCGU CCCCACACAC UGCACAUCGG CGCCACACAC ACACAGGCCC GAAGCGUUUA CUUUGAAA A AAUUAGAGUG UUCAAAGCAG GCCACACACG CCUGGAUACC GCAGCUAGGA AUAAUGGAAU AGGACCGCGG UUCUAUUUU GUUGGUUUUC GGAACUGAGG CCAUGAUUAA GAGGGACGGC CGGGGGCAUU CGUAUUGCGC CGCUAGAGGU GAAAUUCUUG GACCGGCGC AAGACGGACC AGAGCGAAAG CAUUUGCCAA GAAUGUUUUC AUUAAUCAAG AACGAAAGUC GGAGGUUCGA A GACGAUCA GAUACCGUCG UAGUUCCGAC CAUAAACGAU GCCGACUGGC GAUGCGGCGG CGUUAUUCCC AUGACCCGCC GG GCAGCUU CCGGGAAACC AAAGUCUUUG GGUUCCGGGG GGAGUAUGGU UGCAAAGCUG AAACUUAAAG GAAUUGACGG AAG GGCACC ACCAGGAGUG GAGCCUGCGG CUUAAUUUGA CUCAACACGG GAAACCUCAC CCGGCCCGGA CACGGACAGG AUUG ACAGA UUGAUAGCUC UUUCUCGAUU CCGUGGGUGG UGGUGCAUGG CCGUUCUUAG UUGGUGGAGC GAUUUGUCUG GUUAA UUCC GAUAACGAAC GAGACUCUGG CAUGCUAACU AGUUACGCGA CCCACACACG GUCGGCGUAC ACAAACUUCU UAGAGG GAC AAGUGGCGUU CAGCCACCCG AGAUUGAGCA AUAACAGGUC UGUGAUGCCC UUAGAUGUCC GGGGCUGCAC GCGCGCU AC ACUGACUGGC UCAGCGUGUG CCUACCCUAC GCCGGCAGGC GCGGGUAACC CGUUGAACCC CAUUCGUGAU GGGGAUCG G GGAUUGCAAU UAUUCCCCAU GAACGAGGAA UUCCCAGUAA GUGCGGGUCA UAAGCUUGCG UUGAUUAAGU CCCUGCCCU UUGUACACAC CGCCCGUCGC UACUACCGAU UGGAUGGUUU AGUGAGGCCC UCGGAUCGGC CCCGCCGGGG UACACACACA GCCCUGGCG GAGCGCUGAG AAGACGGUCG AACUUGACUA UCUAGAGGAA GUAAAAGUCG UAACAAGGUU UCCGUAGGUG A ACCUGCGG AAGGAUCAUU A

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Macromolecule #85: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 85 / Number of copies: 8 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #86: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 86 / Number of copies: 281 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #87: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER

MacromoleculeName: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / type: ligand / ID: 87 / Number of copies: 1 / Formula: GCP
Molecular weightTheoretical: 521.208 Da
Chemical component information

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

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Macromolecule #88: BLASTICIDIN S

MacromoleculeName: BLASTICIDIN S / type: ligand / ID: 88 / Number of copies: 1 / Formula: BLS
Molecular weightTheoretical: 422.439 Da
Chemical component information

ChemComp-BLS:
BLASTICIDIN S / antibiotic*YM / Blasticidin S

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.63 mg/mL
BufferpH: 7.4
Details: 50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 70 % / Chamber temperature: 288.15 K / Instrument: LEICA EM GP
Details: 30s sample incubation on grid followed by 1.1s blotting time..
Details165nM (OD260nm~10.5)

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Calibrated defocus max: 2.0 µm / Calibrated defocus min: 0.4 µm / Calibrated magnification: 79000 / Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
DetailsCollected in super-resolution mode (0.675A pixel size)
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Number grids imaged: 1 / Number real images: 3500 / Average exposure time: 8.0 sec. / Average electron dose: 41.92 e/Å2
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 730463
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationNumber classes: 5 / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionNumber classes used: 1 / Resolution.type: BY AUTHOR / Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 18937
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

5lzt

Output model

PDB-7nwh:
Mammalian pre-termination 80S ribosome with eRF1 and eRF3 bound by Blasticidin S.

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