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Yorodumi- SASDEJ7: The InvC-ATP synthase, surface presentation of antigens and oxyge... -
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-Basic information
Entry | Database: SASBDB / ID: SASDEJ7 |
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Sample | The InvC-ATP synthase, surface presentation of antigens and oxygen-regulated invasion protein (ISO) complex
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Function / homology | Function and homology information type III protein secretion system complex / bacterial-type flagellum-dependent swarming motility / protein secretion by the type III secretion system / positive chemotaxis / H+-transporting two-sector ATPase / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism / protein transport / ATP binding / cytoplasm Similarity search - Function |
Biological species | Salmonella enterica subsp. enterica serovar Typhimurium (bacteria) |
Citation | Journal: J Mol Biol / Year: 2019 Title: Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. Authors: Ivonne Bernal / Jonathan Börnicke / Johannes Heidemann / Dmitri Svergun / Julia A Horstmann / Marc Erhardt / Anne Tuukkanen / Charlotte Uetrecht / Michael Kolbe / Abstract: Many medically relevant Gram-negative bacteria use the type III secretion system (T3SS) to translocate effector proteins into the host for their invasion and intracellular survival. A multi-protein ...Many medically relevant Gram-negative bacteria use the type III secretion system (T3SS) to translocate effector proteins into the host for their invasion and intracellular survival. A multi-protein complex located at the cytosolic interface of the T3SS is proposed to act as a sorting platform by selecting and targeting substrates for secretion through the system. However, the precise stoichiometry and 3D organization of the sorting platform components are unknown. Here we reconstitute soluble complexes of the Salmonella Typhimurium sorting platform proteins including the ATPase InvC, the regulator OrgB, the protein SpaO and a recently identified subunit SpaO, which we show to be essential for the solubility of SpaO. We establish domain-domain interactions, determine for the first time the stoichiometry of each subunit within the complexes by native mass spectrometry and gain insight into their organization using small-angle X-ray scattering. Importantly, we find that in solution the assembly of SpaO/SpaO/OrgB/InvC adopts an extended L-shaped conformation resembling the sorting platform pods seen in in situ cryo-electron tomography, proposing that this complex is the core building block that can be conceivably assembled into higher oligomers to form the T3SS sorting platform. The determined molecular arrangements of the soluble complexes of the sorting platform provide important insights into its architecture and assembly. |
Contact author |
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-Structure visualization
-Downloads & links
-Data source
SASBDB page | SASDEJ7 |
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-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-External links
Related items in Molecule of the Month |
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-Models
-Sample
Sample | Name: The InvC-ATP synthase, surface presentation of antigens and oxygen-regulated invasion protein (ISO) complex Specimen concentration: 1 mg/ml / Entity id: 824 / 825 / 1359 / 1360 |
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Buffer | Name: 10 mM Tris-HCl, 50 mM NaCl / pH: 8 |
Entity #824 | Name: SpaOc / Type: protein Description: Surface presentation of antigens protein SpaO SpaO(SPOA2) Formula weight: 12.374 / Num. of mol.: 2 Source: Salmonella enterica subsp. enterica serovar Typhimurium References: UniProt: P40699 Sequence: METLDIQHIE EENNTTETAE TLPGLNQLPV KLEFVLYRKN VTLAELEAMG QQQLLSLPTN AELNVEIMAN GVLLGNGELV QMNDTLGVEI HEWLSESGNG ESAWSHPQFE K |
Entity #825 | Name: SpaO / Type: protein Description: Surface presentation of antigens protein SpaO(SPOA1,2) Formula weight: 33.793 / Num. of mol.: 1 Source: Salmonella enterica subsp. enterica serovar Typhimurium References: UniProt: P40699 Sequence: MSLRVRQIDR REWLLAQTAT ECQRHGREAT LEYPTRQGMW VRLSDAEKRW SAWIKPGDWL EHVSPALAGA AVSAGAEHLV VPWLAATERP FELPVPHLSC RRLCVENPVP GSALPEGKLL HIMSDRGGLW FEHLPELPAV GGGRPKMLRW PLRFVIGSSD TQRSLLGRIG ...Sequence: MSLRVRQIDR REWLLAQTAT ECQRHGREAT LEYPTRQGMW VRLSDAEKRW SAWIKPGDWL EHVSPALAGA AVSAGAEHLV VPWLAATERP FELPVPHLSC RRLCVENPVP GSALPEGKLL HIMSDRGGLW FEHLPELPAV GGGRPKMLRW PLRFVIGSSD TQRSLLGRIG IGDVLLIRTS RAEVYCYAKK LGHFNRVEGG IIVETLDIQH IEEENNTTET AETLPGLNQL PVKLEFVLYR KNVTLAELEA MGQQQLLSLP TNAELNVEIM ANGVLLGNGE LVQMNDTLGV EIHEWLSESG NGE |
Entity #1359 | Name: OrgB / Type: protein / Description: Oxygen-regulated invasion protein OrgB / Formula weight: 26.46 / Num. of mol.: 2 Source: Salmonella enterica subsp. enterica serovar Typhimurium References: UniProt: P0CL45 Sequence: MLKNIPIPSP LSPVEGILIK RKTLERYFSI ERLEQQAHQR AKRILREAEE EAKTLRMYAY QEGYEQGMID ALQQVAAYLT DNQTMAWKWM EKIQIYAREL FSAAVDHPET LLTVLDEWLR DFDKPEGQLF LTLPVNAKKD HQKLMVLLME NWPGTFNLKY HQEQRFIMSC ...Sequence: MLKNIPIPSP LSPVEGILIK RKTLERYFSI ERLEQQAHQR AKRILREAEE EAKTLRMYAY QEGYEQGMID ALQQVAAYLT DNQTMAWKWM EKIQIYAREL FSAAVDHPET LLTVLDEWLR DFDKPEGQLF LTLPVNAKKD HQKLMVLLME NWPGTFNLKY HQEQRFIMSC GDQIAEFSPE QFVETAVGVI KHHLDELPQD CRTISDNAIN ALIDEWKTKT QAEVIR |
Entity #1360 | Name: InvC / Type: protein / Description: ATP synthase InvC / Formula weight: 47.61 / Num. of mol.: 1 Source: Salmonella enterica subsp. enterica serovar Typhimurium References: UniProt: E8XL22 Sequence: MKTPRLLQYL AYPQKITGPI IEAELRDVAI GELCEIRRGW HQKQVVARAQ VVGLQRERTV LSLIGNAQGL SRDVVLYPTG RALSAWVGYS VLGAVLDPTG KIVERFTPEV APISEERVID VAPPSYASRV GVREPLITGV RAIDGLLTCG VGQRMGIFAS AGCGKTMLMH ...Sequence: MKTPRLLQYL AYPQKITGPI IEAELRDVAI GELCEIRRGW HQKQVVARAQ VVGLQRERTV LSLIGNAQGL SRDVVLYPTG RALSAWVGYS VLGAVLDPTG KIVERFTPEV APISEERVID VAPPSYASRV GVREPLITGV RAIDGLLTCG VGQRMGIFAS AGCGKTMLMH MLIEQTEADV FVIGLIGERG REVTEFVDML RASHKKEKCV LVFATSDFPS VDRCNAAQLA TTVAEYFRDQ GKRVVLFIDS MTRYARALRD VALASGERPA RRGYPASVFD NLPRLLERPG ATSEGSITAF YTVLLESEEE ADPMADEIRS ILDGHLYLSR KLAGQGHYPA IDVLKSVSRV FGQVTTPTHA EQASAVRKLM TRLEELQLFI DLGEYRPGEN IDNDRAMQMR DSLKAWLCQP VAQYSSFDDT LSGMNAFADQ N |
-Experimental information
Beam | Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm | ||||||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 2M | ||||||||||||||||||||||||||||||||||||
Scan | Title: The InvC-ATP synthase, surface presentation of antigens and oxygen-regulated invasion protein (ISO) complex Measurement date: Apr 12, 2017 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 3600 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 442 /
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Result | Type of curve: sec Comments: SEC-SAXS was performed at 20°C using the following parameters: Column type, XXXX; Flow rate: XXXX mL/min; Total acquisition time: 60 min (3600 x 1 s frames); Injection concentration: XXXX ...Comments: SEC-SAXS was performed at 20°C using the following parameters: Column type, XXXX; Flow rate: XXXX mL/min; Total acquisition time: 60 min (3600 x 1 s frames); Injection concentration: XXXX mg/mL; Injection volume: XXXX μL.
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