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TitleMolecular Organization of Soluble Type III Secretion System Sorting Platform Complexes.
Journal, issue, pagesJ Mol Biol, Vol. 431, Issue 19, Page 3787-3803, Year 2019
Publish dateSep 6, 2019
AuthorsIvonne Bernal / Jonathan Börnicke / Johannes Heidemann / Dmitri Svergun / Julia A Horstmann / Marc Erhardt / Anne Tuukkanen / Charlotte Uetrecht / Michael Kolbe /
PubMed AbstractMany medically relevant Gram-negative bacteria use the type III secretion system (T3SS) to translocate effector proteins into the host for their invasion and intracellular survival. A multi-protein ...Many medically relevant Gram-negative bacteria use the type III secretion system (T3SS) to translocate effector proteins into the host for their invasion and intracellular survival. A multi-protein complex located at the cytosolic interface of the T3SS is proposed to act as a sorting platform by selecting and targeting substrates for secretion through the system. However, the precise stoichiometry and 3D organization of the sorting platform components are unknown. Here we reconstitute soluble complexes of the Salmonella Typhimurium sorting platform proteins including the ATPase InvC, the regulator OrgB, the protein SpaO and a recently identified subunit SpaO, which we show to be essential for the solubility of SpaO. We establish domain-domain interactions, determine for the first time the stoichiometry of each subunit within the complexes by native mass spectrometry and gain insight into their organization using small-angle X-ray scattering. Importantly, we find that in solution the assembly of SpaO/SpaO/OrgB/InvC adopts an extended L-shaped conformation resembling the sorting platform pods seen in in situ cryo-electron tomography, proposing that this complex is the core building block that can be conceivably assembled into higher oligomers to form the T3SS sorting platform. The determined molecular arrangements of the soluble complexes of the sorting platform provide important insights into its architecture and assembly.
External linksJ Mol Biol / PubMed:31288030
MethodsSAS (X-ray synchrotron)
Structure data

SASDC68: Surface presentation of antigens protein, SpaOc (Surface presentation of antigens protein SpaO SpaO(SPOA2), SpaOc)
Method: SAXS/SANS

SASDC78: Surface presentation of antigens protein SpaOc-SpaO(SPOA1,2)
Method: SAXS/SANS

SASDC88: Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus
Method: SAXS/SANS

SASDC98: Surface presentation of antigens protein SpaOc-SpaO N-terminus
Method: SAXS/SANS

SASDEJ7: The InvC-ATP synthase, surface presentation of antigens and oxygen-regulated invasion protein (ISO) complex
Method: SAXS/SANS

SASDEK7: SpaO C-terminus (Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus)
Method: SAXS/SANS

Source
  • Salmonella enterica subsp. enterica serovar typhimurium (bacteria)

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