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TitleStructure of mechanically activated ion channel OSCA2.3 reveals mobile elements in the transmembrane domain.
Journal, issue, pagesStructure, Vol. 32, Issue 2, Page 157-167.e5, Year 2024
Publish dateFeb 1, 2024
AuthorsSebastian Jojoa-Cruz / Batuujin Burendei / Wen-Hsin Lee / Andrew B Ward /
PubMed AbstractMembers of the OSCA/TMEM63 family are mechanically activated ion channels and structures of some OSCA members have revealed the architecture of these channels and structural features that are ...Members of the OSCA/TMEM63 family are mechanically activated ion channels and structures of some OSCA members have revealed the architecture of these channels and structural features that are potentially involved in mechanosensation. However, these structures are all in a similar state and information about the motion of different elements of the structure is limited, preventing a deeper understanding of how these channels work. Here, we used cryoelectron microscopy to determine high-resolution structures of Arabidopsis thaliana OSCA1.2 and OSCA2.3 in peptidiscs. The structure of OSCA1.2 matches previous structures of the same protein in different environments. Yet, in OSCA2.3, the TM6a-TM7 linker adopts a different conformation that constricts the pore on its cytoplasmic side. Furthermore, coevolutionary sequence analysis uncovered a conserved interaction between the TM6a-TM7 linker and the beam-like domain (BLD). Our results reveal conformational heterogeneity and differences in conserved interactions between the TMD and BLD among members of the OSCA family.
External linksStructure / PubMed:38103547 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 2.8 Å
Structure data

41043

8t56

EMDB-41043, PDB-8t56:
Structure of mechanically activated ion channel OSCA1.2 in peptidiscs
Method: EM (single particle) / Resolution: 2.8 Å

41044

8t57

EMDB-41044, PDB-8t57:
Structure of mechanically activated ion channel OSCA2.3 in peptidiscs
Method: EM (single particle) / Resolution: 2.7 Å

Chemicals

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

ChemComp-PLM:
PALMITIC ACID / Palmitic acid

ChemComp-LBN:
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / phospholipid*YM / POPC

ChemComp-CLR:
CHOLESTEROL / Cholesterol

Source
  • arabidopsis thaliana (thale cress)
  • synthetic construct (others)
KeywordsMEMBRANE PROTEIN / mechanically activated ion channel

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