Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complex.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 120, Issue 29, Page e2301199120, Year 2023
Publish date	Jul 18, 2023
Authors	Jenny Jiou / Joy M Shaffer / Natalia E Bernades / Ho Yee Joyce Fung / Juliana Kikumoto Dias / Sheena D'Arcy / Yuh Min Chook /
PubMed Abstract	Previously, we showed that the nuclear import receptor Importin-9 wraps around the H2A-H2B core to chaperone and transport it from the cytoplasm to the nucleus. However, unlike most nuclear import ...Previously, we showed that the nuclear import receptor Importin-9 wraps around the H2A-H2B core to chaperone and transport it from the cytoplasm to the nucleus. However, unlike most nuclear import systems where RanGTP dissociates cargoes from their importins, RanGTP binds stably to the Importin-9•H2A-H2B complex, and formation of the ternary RanGTP•Importin-9•H2A-H2B complex facilitates H2A-H2B release to the assembling nucleosome. It was unclear how RanGTP and the cargo H2A-H2B can bind simultaneously to an importin, and how interactions of the three components position H2A-H2B for release. Here, we show cryo-EM structures of Importin-9•RanGTP and of its yeast homolog Kap114, including Kap114•RanGTP, Kap114•H2A-H2B, and RanGTP•Kap114•H2A-H2B, to explain how the conserved Kap114 binds H2A-H2B and RanGTP simultaneously and how the GTPase primes histone transfer to the nucleosome. In the ternary complex, RanGTP binds to the N-terminal repeats of Kap114 in the same manner as in the Kap114/Importin-9•RanGTP complex, and H2A-H2B binds via its acidic patch to the Kap114 C-terminal repeats much like in the Kap114/Importin-9•H2A-H2B complex. Ran binds to a different conformation of Kap114 in the ternary RanGTP•Kap114•H2A-H2B complex. Here, Kap114 no longer contacts the H2A-H2B surface proximal to the H2A docking domain that drives nucleosome assembly, positioning it for transfer to the assembling nucleosome or to dedicated H2A-H2B chaperones in the nucleus.
External links	Proc Natl Acad Sci U S A / PubMed:37450495 / PubMed Central
Methods	EM (single particle)
Resolution	3.21 - 3.78 Å
Structure data	28782 8f0x EMDB-28782, PDB-8f0x: Cryo-EM structure of Kap114 bound to H2A-H2B Method: EM (single particle) / Resolution: 3.21 Å 28788 8f19 EMDB-28788, PDB-8f19: Cryo-EM structure of Kap114 bound to Gsp1 (RanGTP) Method: EM (single particle) / Resolution: 3.49 Å 28796 8f1e EMDB-28796, PDB-8f1e: Cryo-EM structure of Kap114 bound to Gsp1 (RanGTP) and H2A-H2B Method: EM (single particle) / Resolution: 3.28 Å 28899 8f7a EMDB-28899, PDB-8f7a: Cryo-EM structure of Importin-9 bound to RanGTP Method: EM (single particle) / Resolution: 3.78 Å
Chemicals	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM / Guanosine triphosphate ChemComp-MG*: Unknown entry
Source	saccharomyces cerevisiae s288c (yeast) homo sapiens (human) saccharomyces cerevisiae (brewer's yeast)
Keywords	PROTEIN TRANSPORT/STRUCTURAL PROTEIN / Karyopherin Beta Nuclear Transport Histone Histone Chaperone / PROTEIN TRANSPORT-STRUCTURAL PROTEIN complex / PROTEIN TRANSPORT/NUCLEAR PROTEIN / Karyopherin Beta / Nuclear Transport / GTPase / PROTEIN TRANSPORT-NUCLEAR PROTEIN complex / PROTEIN TRANSPORT/STRUCTURAL PROTEIN/NUCLEAR PROTEIN / Histone Chaperone / Histones / PROTEIN TRANSPORT-STRUCTURAL PROTEIN-NUCLEAR PROTEIN complex / PROTEIN TRANSPORT / Nuclear Import / Importin / RanGTP