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TitleHigh-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 7241, Year 2022
Publish dateNov 24, 2022
AuthorsRuochen Ouyang / Ana Rita Costa / C Keith Cassidy / Aleksandra Otwinowska / Vera C J Williams / Agnieszka Latka / Phill J Stansfeld / Zuzanna Drulis-Kawa / Yves Briers / Daniël M Pelt / Stan J J Brouns / Ariane Briegel /
PubMed AbstractThe Klebsiella jumbo myophage ϕKp24 displays an unusually complex arrangement of tail fibers interacting with a host cell. In this study, we combine cryo-electron microscopy methods, protein ...The Klebsiella jumbo myophage ϕKp24 displays an unusually complex arrangement of tail fibers interacting with a host cell. In this study, we combine cryo-electron microscopy methods, protein structure prediction methods, molecular simulations, microbiological and machine learning approaches to explore the capsid, tail, and tail fibers of ϕKp24. We determine the structure of the capsid and tail at 4.1 Å and 3.0 Å resolution. We observe the tail fibers are branched and rearranged dramatically upon cell surface attachment. This complex configuration involves fourteen putative tail fibers with depolymerase activity that provide ϕKp24 with the ability to infect a broad panel of capsular polysaccharide (CPS) types of Klebsiella pneumoniae. Our study provides structural and functional insight into how ϕKp24 adapts to the variable surfaces of capsulated bacterial pathogens, which is useful for the development of phage therapy approaches against pan-drug resistant K. pneumoniae strains.
External linksNat Commun / PubMed:36433970 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution3.0 - 4.3 Å
Structure data

13862

8bfl

8bfp

EMDB-13862: Jumbo Phage phi-Kp24 empty capsid
PDB-8bfl: Jumbo Phage phi-kp24 empty capsid hexamers
PDB-8bfp: Jumbo Phage phi-kp24 empty capsid pentamer hexamers
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-14356: Jumbo Phage phi-Kp24 full capsid
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-14357: Jumbo Phage phi-Kp24 extended tail
Method: EM (single particle) / Resolution: 4.08 Å

15669

8au1

8bfk

EMDB-15669, PDB-8au1:
Jumbo Phage phi-kp24 tail outer sheath
PDB-8bfk: Jumbo Phage phi-kp24 tail inner tube
Method: EM (helical sym.) / Resolution: 3.0 Å

Source
  • klebsiella phage vb_kpm_fbkp24 (virus)
KeywordsSTRUCTURAL PROTEIN / Jumbo Phage / Klebsiella pneumoniae / tail / sheath / VIRUS / Klebsiella pheumoniae / Capsid

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