EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and genome ejection mechanism of phage P68.
ジャーナル・号・ページ	Sci Adv, Vol. 5, Issue 10, Page eaaw7414, Year 2019
掲載日	2019年10月16日
著者	Dominik Hrebík / Dana Štveráková / Karel Škubník / Tibor Füzik / Roman Pantůček / Pavel Plevka /
PubMed 要旨	Phages infecting can be used as therapeutics against antibiotic-resistant bacterial infections. However, there is limited information about the mechanism of genome delivery of phages that infect ...Phages infecting can be used as therapeutics against antibiotic-resistant bacterial infections. However, there is limited information about the mechanism of genome delivery of phages that infect Gram-positive bacteria. Here, we present the structures of native phage P68, genome ejection intermediate, and empty particle. The P68 head contains 72 subunits of inner core protein, 15 of which bind to and alter the structure of adjacent major capsid proteins and thus specify attachment sites for head fibers. Unlike in the previously studied phages, the head fibers of P68 enable its virion to position itself at the cell surface for genome delivery. The unique interaction of one end of P68 DNA with one of the 12 portal protein subunits is disrupted before the genome ejection. The inner core proteins are released together with the DNA and enable the translocation of phage genome across the bacterial membrane into the cytoplasm.
リンク	Sci Adv / PubMed:31663016 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2 - 22.7 Å
構造データ	4435 6iac EMDB-4435, PDB-6iac: Portal and tail of native bacteriophage P68 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-4436: Five-fold symmetrized map of native bacteriophage P68 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-4437: Asymmetric map of native bacteriophage P68 手法: EM (単粒子) / 解像度: 4.7 Å 4438 6iat EMDB-4438, PDB-6iat: Icosahedrally averaged capsid of bacteriophage P68 手法: EM (単粒子) / 解像度: 3.3 Å 4440 6iaw EMDB-4440, PDB-6iaw: Structure of head fiber and inner core protein gp22 of native bacteriophage P68 手法: EM (単粒子) / 解像度: 3.8 Å 4442 6ib1 EMDB-4442, PDB-6ib1: Icosahedrally averaged capsid of empty particle of bacteriophage P68 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-4449: Localized reconstruction of tailknob of native bacteriophage P68 手法: EM (単粒子) / 解像度: 11.3 Å EMDB-4450: Localized reconstruction of tail-spike of bacteriophage P68 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-4451: Icosahedrally averaged genome release intermediate capsid of bacteriophage P68 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-4453: Five-fold averaged reconstruction of the genome release intermediate bacteriophage P68 手法: EM (単粒子) / 解像度: 22.7 Å EMDB-4454: Dodecameric reconstruction of portal and tail of genome release intermediate state of bacteriophage P68 手法: EM (単粒子) / 解像度: 18.8 Å EMDB-4455: Dodecameric reconstruction of portal and tail of genome release intermediate state of bacteriophage P68 (subset with tailknob) 手法: EM (単粒子) / 解像度: 22.7 Å EMDB-4456: Five-fold symmetric reconstruction of empty particle of bacteriophage P68 手法: EM (単粒子) / 解像度: 4.28 Å EMDB-4457: Dodecameric reconstruction of portal and tail of empty particle of bacteriophage P68 (subset without tailknob) 手法: EM (単粒子) / 解像度: 7.1 Å EMDB-4458: Dodecameric reconstruction of portal and tail of empty partice of bacteriophage P68 (subset with tailknob) 手法: EM (単粒子) / 解像度: 21.7 Å 4459 6q3g EMDB-4459, PDB-6q3g: Structure of native bacteriophage P68 手法: EM (単粒子) / 解像度: 3.8 Å PDB-6iab: Tail fiber of Staphylococcus aureus phage P68 手法: X-RAY DIFFRACTION / 解像度: 2.0 Å
化合物	ChemComp-HOH: WATER / 水
由来	staphylococcus phage p68 (ファージ) staphylococcus aureus phage p68 (ファージ)
キーワード	SUGAR BINDING PROTEIN / structural protein (タンパク質) / receptor binding protein (受容体) / beta-propeller (Βプロペラドメイン) / structural proteins (構造) / bacteriophage portal / bacteriophage tail / native complex / bacteriophage capsid / HK97 fold / bacteriophage (ファージ) / head fiber / inner core protein (内核) / VIRUS (ウイルス) / complex