Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Euglena's atypical respiratory chain adapts to the discoidal cristae and flexible metabolism.
Journal, issue, pages	Nat Commun, Vol. 15, Issue 1, Page 1628, Year 2024
Publish date	Feb 22, 2024
Authors	Zhaoxiang He / Mengchen Wu / Hongtao Tian / Liangdong Wang / Yiqi Hu / Fangzhu Han / Jiancang Zhou / Yong Wang / Long Zhou /
PubMed Abstract	Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron ...Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron transport chain. While structures of the electron transport chain complexes and supercomplexes of most other eukaryotic clades have been reported, no similar structure is currently available for Discoba, limiting the understandings of its core metabolism and leaving a gap in the evolutionary tree of eukaryotic bioenergetics. Here, we report high-resolution cryo-EM structures of Euglena's respirasome I + III + IV and supercomplex III + IV. A previously unreported fatty acid synthesis domain locates on the tip of complex I's peripheral arm, providing a clear picture of its atypical subunit composition identified previously. Individual complexes are re-arranged in the respirasome to adapt to the non-uniform membrane curvature of the discoidal cristae. Furthermore, Euglena's conformationally rigid complex I is deactivated by restricting ubiquinone's access to its substrate tunnel. Our findings provide structural insights for therapeutic developments against euglenozoan parasite infections.
External links	Nat Commun / PubMed:38388527 / PubMed Central
Methods	EM (single particle)
Resolution	2.69 - 3.14 Å
Structure data	EMDB-35662: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex I peripheral arm focused Method: EM (single particle) / Resolution: 2.73 Å EMDB-35663: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex I proximal membrane arm focused Method: EM (single particle) / Resolution: 2.72 Å EMDB-35664: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex I distal membrane arm focused Method: EM (single particle) / Resolution: 2.69 Å EMDB-35665: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex III2 focused Method: EM (single particle) / Resolution: 2.77 Å EMDB-35666: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex IV focused Method: EM (single particle) / Resolution: 2.76 Å EMDB-35667: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, complex III2 focused Method: EM (single particle) / Resolution: 2.85 Å EMDB-35668: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, complex IV focused Method: EM (single particle) / Resolution: 2.89 Å EMDB-35669: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, complex IV focused Method: EM (single particle) / Resolution: 3.14 Å 35720 8iuf EMDB-35720, PDB-8iuf: Cryo-EM structure of Euglena gracilis super-complex I+III2+IV, composite Method: EM (single particle) / Resolution: 2.81 Å 35723 8iuj EMDB-35723: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, composite PDB-8iuj: Cryo-EM structure of Euglena gracilis super-complex III2+IV2, composite Method: EM (single particle) / Resolution: 3.06 Å EMDB-35819: Cryo-EM map of Euglena gracilis respirasome I+III2+IV Method: EM (single particle) / Resolution: 2.81 Å EMDB-35820: Cryo-EM map of Euglena gracilis supercomplex III2+IV2 Method: EM (single particle) / Resolution: 3.06 Å EMDB-36094: Cryo-EM map of Euglena gracilis respiratory complex I in the deactive state, peripheral arm focused Method: EM (single particle) / Resolution: 3.07 Å EMDB-36096: Cryo-EM map of Euglena gracilis respiratory complex I in the deactive state, proximal membrane arm focused Method: EM (single particle) / Resolution: 3.0 Å EMDB-36097: Cryo-EM map of Euglena gracilis respiratory complex I in the deactive state, distal membrane arm focused Method: EM (single particle) / Resolution: 3.03 Å EMDB-36098: Cryo-EM consensus map of Euglena gracilis respiratory complex I, deactive state Method: EM (single particle) / Resolution: 3.11 Å EMDB-36099: Cryo-EM map of Euglena gracilis respiratory complex I in the turnover state, peripheral arm focused Method: EM (single particle) / Resolution: 2.82 Å EMDB-36100: Cryo-EM map of Euglena gracilis respiratory complex I in the turnover state, proximal membrane arm focused Method: EM (single particle) / Resolution: 2.87 Å EMDB-36101: Cryo-EM map of Euglena gracilis respiratory complex I in the turnover state, distal membrane arm focused Method: EM (single particle) / Resolution: 2.89 Å EMDB-36102: Cryo-EM consensus map of Euglena gracilis respiratory complex I, turnover state Method: EM (single particle) / Resolution: 2.87 Å EMDB-36103: Cryo-EM map of Euglena gracilis respiratory complex I in the NADH state, peripheral arm focused Method: EM (single particle) / Resolution: 2.97 Å EMDB-36104: Cryo-EM map of Euglena gracilis respiratory complex I in the NADH state, proximal membrane arm focused Method: EM (single particle) / Resolution: 2.93 Å EMDB-36105: Cryo-EM map of Euglena gracilis respiratory complex I in the NADH state, distal membrane focused Method: EM (single particle) / Resolution: 2.94 Å EMDB-36106: Cryo-EM consensus map of Euglena gracilis respiratory complex I, NADH state Method: EM (single particle) / Resolution: 3.03 Å 36107 8j9h EMDB-36107: Cryo-EM composite map of Euglena gracilis respiratory complex I, deactive state PDB-8j9h: Cryo-EM structure of Euglena gracilis respiratory complex I, deactive state Method: EM (single particle) / Resolution: 3.11 Å 36108 8j9i EMDB-36108: Cryo-EM composite map of Euglena gracilis complex I, turnover state PDB-8j9i: Cryo-EM structure of Euglena gracilis complex I, turnover state Method: EM (single particle) / Resolution: 2.87 Å 36109 8j9j EMDB-36109: Cryo-EM composite map of Euglena gracilis complex I, NADH state PDB-8j9j: Cryo-EM structure of Euglena gracilis complex I, NADH state Method: EM (single particle) / Resolution: 3.03 Å
Chemicals	ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster ChemComp-SF4: IRON/SULFUR CLUSTER / Iron–sulfur cluster ChemComp-K: Unknown entry ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipidYM / Phosphatidylcholine ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipidYM / Phosphatidylethanolamine ChemComp-S12: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine ChemComp-CDL: CARDIOLIPIN / phospholipidYM / Cardiolipin ChemComp-ZN: Unknown entry ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate ChemComp-ZMP: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate ChemComp-HEA: HEME-A / Heme A ChemComp-CU: COPPER (II) ION / Copper ChemComp-MG: Unknown entry ChemComp-PX2: 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE ChemComp-U10: UBIQUINONE-10 / Coenzyme Q10 ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE / Heme B ChemComp-HEC: HEME C / Heme C ChemComp-FMN: FLAVIN MONONUCLEOTIDE / Flavin mononucleotide ChemComp-NAI*: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / Nicotinamide adenine dinucleotide
Source	euglena gracilis (euglena)
Keywords	ELECTRON TRANSPORT / Electron transport chain / supercomplex / membrane protein / Euglena gracilis / complex / respiratory complex