EMDB-35720: Cryo-EM structure of Euglena gracilis super-complex I+III2+IV, co...

Basic information

Entry

Database: EMDB / ID: EMD-35720

• Title: Cryo-EM structure of Euglena gracilis super-complex I+III2+IV, composite

Sample

• Complex: Euglena gracilis respirasome supercomplex I+III2+IV
  • Complex: Euglena gracilis respirasome supercomplex I+III2+IV
    • Protein or peptide: x 89 types
• Protein or peptide: x 1 types
• Ligand: x 18 types

• Keywords: Electron transport chain / supercomplex / membrane protein / Euglena gracilis / ELECTRON TRANSPORT

Function / homology

Function:

cytochrome-c oxidase / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / oxidative phosphorylation / cytochrome-c oxidase activity / NADH:ubiquinone reductase (H+-translocating) / NADH dehydrogenase (ubiquinone) activity / ATP synthesis coupled electron transport / respirasome / respiratory electron transport chain / mitochondrial membrane / mitochondrial inner membrane / electron transfer activity / copper ion binding / heme binding / mitochondrion / membrane / metal ion binding

Domain/homology:

Cytochrome C oxidase subunit II, transmembrane domain / Cytochrome c oxidase subunit III-like superfamily / Cytochrome c1, transmembrane anchor, C-terminal / Cytochrome C oxidase subunit II, transmembrane domain / Cytochrome oxidase subunit II transmembrane region profile. / Cytochrome c/quinol oxidase subunit II / Cytochrome c1 / Cytochrome C1 family / Cytochrome b/b6, C-terminal / Cytochrome b(C-terminal)/b6/petD / Cytochrome b/b6 C-terminal region profile. / Cytochrome b/b6, C-terminal domain superfamily / Cytochrome b/b6/petB / Copper centre Cu(A) / CO II and nitrous oxide reductase dinuclear copper centers signature. / Cytochrome C oxidase subunit II, transmembrane domain superfamily / Cytochrome c oxidase, subunit I, copper-binding site / Heme-copper oxidase catalytic subunit, copper B binding region signature. / Cytochrome c oxidase-like, subunit I domain / Cytochrome oxidase subunit I profile. / Cytochrome b/b6, N-terminal / Cytochrome b/b6-like domain superfamily / Cytochrome b/b6 N-terminal region profile. / Cytochrome c oxidase subunit I / Cytochrome c oxidase-like, subunit I superfamily / Cytochrome C and Quinol oxidase polypeptide I / Di-haem cytochrome, transmembrane / Cytochrome C oxidase subunit II, periplasmic domain / Cytochrome c oxidase subunit II-like C-terminal / Cytochrome oxidase subunit II copper A binding domain profile. / Peptidase M16, N-terminal / Insulinase (Peptidase family M16) / Metalloenzyme, LuxS/M16 peptidase-like / NADH:ubiquinone oxidoreductase / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / Proton-conducting membrane transporter / Cytochrome c-like domain superfamily / Cupredoxin

Component:

Cytochrome b / NADH-ubiquinone oxidoreductase chain 4 / Cytochrome c1, heme protein / Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial / Ubiquinol-cytochrome-C reductase complex subunit IX, mitochondrial / Cytochrome c oxidase subunit 1 / Putative NADH dehydrogenase subunit 6 / Cytochrome c oxidase subunit 2

• Biological species: Euglena gracilis (euglena)
• Method: single particle reconstruction / cryo EM / Resolution: 2.81 Å
• Authors: Wu MC / Tian HT / He ZX / Hu YQ / Zhou L

Funding support

China, 1 items

Organization	Grant number	Country
Other government	ZJU100 Young Professor	China

• Citation: Journal: Nat Commun / Year: 2024; Title: Euglena's atypical respiratory chain adapts to the discoidal cristae and flexible metabolism.; Authors: Zhaoxiang He / Mengchen Wu / Hongtao Tian / Liangdong Wang / Yiqi Hu / Fangzhu Han / Jiancang Zhou / Yong Wang / Long Zhou / ; Abstract: Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron transport chain. While structures of the electron transport chain complexes and supercomplexes of most other eukaryotic clades have been reported, no similar structure is currently available for Discoba, limiting the understandings of its core metabolism and leaving a gap in the evolutionary tree of eukaryotic bioenergetics. Here, we report high-resolution cryo-EM structures of Euglena's respirasome I + III + IV and supercomplex III + IV. A previously unreported fatty acid synthesis domain locates on the tip of complex I's peripheral arm, providing a clear picture of its atypical subunit composition identified previously. Individual complexes are re-arranged in the respirasome to adapt to the non-uniform membrane curvature of the discoidal cristae. Furthermore, Euglena's conformationally rigid complex I is deactivated by restricting ubiquinone's access to its substrate tunnel. Our findings provide structural insights for therapeutic developments against euglenozoan parasite infections.

History

Deposition	Mar 24, 2023	-
Header (metadata) release	Feb 28, 2024	-
Map release	Feb 28, 2024	-
Update	Mar 6, 2024	-
Current status	Mar 6, 2024	Processing site: PDBc / Status: Released

Map

• File: Download / File: emd_35720.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.2 Å

Density

Contour Level	By AUTHOR: 5.0
Minimum - Maximum	-25.80301 - 37.68947
Average (Standard dev.)	-0.002453877 (±1.0491005)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 0 0 0 Dimensions 448 448 448 Spacing 448 448 448
Cell	A=B=C: 537.60004 Å α=β=γ: 90.0 °

Supplemental data

Half map: #2

• File: emd_35720_half_map_1.map

Half map: #1

• File: emd_35720_half_map_2.map

Sample components

Entire : Euglena gracilis respirasome supercomplex I+III2+IV

• Entire: Name: Euglena gracilis respirasome supercomplex I+III2+IV

Components

• Complex: Euglena gracilis respirasome supercomplex I+III2+IV
  • Complex: Euglena gracilis respirasome supercomplex I+III2+IV
    • Protein or peptide: NDUFS1a
    • Protein or peptide: NDUFS1b
    • Protein or peptide: ND2b
    • Protein or peptide: COXEG1
    • Protein or peptide: COXEG3
    • Protein or peptide: COXEG4
    • Protein or peptide: COXEG5
    • Protein or peptide: COXEG6
    • Protein or peptide: COXEG7
    • Protein or peptide: COXEG8
    • Protein or peptide: COXEG9
    • Protein or peptide: COXEG10
    • Protein or peptide: ND4L
    • Protein or peptide: COX5b-2
    • Protein or peptide: COX5c
    • Protein or peptide: COX6a
    • Protein or peptide: COX6b-1
    • Protein or peptide: COX7a
    • Protein or peptide: COX7c
    • Protein or peptide: NDUFA1NADH dehydrogenase (ubiquinone), alpha 1
    • Protein or peptide: NDUFA2
    • Protein or peptide: NDUFA3
    • Protein or peptide: NDUFA5
    • Protein or peptide: NDUFA6
    • Protein or peptide: NDUFA7
    • Protein or peptide: NDUFA8
    • Protein or peptide: NDUFA9
    • Protein or peptide: NDUFAB1-alpha
    • Protein or peptide: NDUFAB1-beta
    • Protein or peptide: NDUFA12
    • Protein or peptide: NDUFA13
    • Protein or peptide: NDUFA11
    • Protein or peptide: NDUFB2
    • Protein or peptide: NDUFB3
    • Protein or peptide: NDUFB4
    • Protein or peptide: NDUFB5
    • Protein or peptide: NDUFB6
    • Protein or peptide: NDUFB7
    • Protein or peptide: NDUFB8
    • Protein or peptide: NDUFB9
    • Protein or peptide: NDUFB10
    • Protein or peptide: NDUFB11
    • Protein or peptide: Cytochrome c oxidase subunit 1
    • Protein or peptide: Cytochrome c oxidase subunit 2
    • Protein or peptide: Putative NADH dehydrogenase subunit 6
    • Protein or peptide: NDUFC2
    • Protein or peptide: COX4
    • Protein or peptide: NDUEG1
    • Protein or peptide: NDUEG2
    • Protein or peptide: NDUEG3
    • Protein or peptide: NDUEG4
    • Protein or peptide: NDUEG5
    • Protein or peptide: NDUEG6
    • Protein or peptide: NDUEG7
    • Protein or peptide: NDUEG8
    • Protein or peptide: NDUEG9
    • Protein or peptide: NDUEG10
    • Protein or peptide: NDUEG11
    • Protein or peptide: NDUEG11
    • Protein or peptide: NDUEG13
    • Protein or peptide: NDUFX
    • Protein or peptide: NDUCA1
    • Protein or peptide: NDUCA2
    • Protein or peptide: NDUCA3
    • Protein or peptide: ND1
    • Protein or peptide: ND2a
    • Protein or peptide: ND3
    • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
    • Protein or peptide: ND5
    • Protein or peptide: MPP-beta
    • Protein or peptide: Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial
    • Protein or peptide: Cytochrome b
    • Protein or peptide: Cytochrome c1, heme protein
    • Protein or peptide: UQCRFS1
    • Protein or peptide: UQCRH
    • Protein or peptide: UQCRB
    • Protein or peptide: UQCRQ
    • Protein or peptide: UQCR9
    • Protein or peptide: UQCR10
    • Protein or peptide: Ubiquinol-cytochrome-C reductase complex subunit IX, mitochondrialCoenzyme Q – cytochrome c reductase
    • Protein or peptide: NDUFS2
    • Protein or peptide: NDUFS3
    • Protein or peptide: NDUFS4
    • Protein or peptide: NDUFS5
    • Protein or peptide: NDUFS6
    • Protein or peptide: NDUFS7
    • Protein or peptide: NDUFS8
    • Protein or peptide: NDUFV1
    • Protein or peptide: NDUFV2
• Protein or peptide: poly(UNK)
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
• Ligand: POTASSIUM IONPotassium
• Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
• Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
• Ligand: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine
• Ligand: CARDIOLIPIN
• Ligand: ZINC ION
• Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
• Ligand: HEME-A
• Ligand: COPPER (II) ION
• Ligand: MAGNESIUM ION
• Ligand: 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE
• Ligand: UBIQUINONE-10Coenzyme Q10
• Ligand: PROTOPORPHYRIN IX CONTAINING FE
• Ligand: HEME C
• Ligand: FLAVIN MONONUCLEOTIDE

Supramolecule #1: Euglena gracilis respirasome supercomplex I+III2+IV

• Supramolecule: Name: Euglena gracilis respirasome supercomplex I+III2+IV / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#89
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 2.5 MDa

Supramolecule #2: Euglena gracilis respirasome supercomplex I+III2+IV

• Supramolecule: Name: Euglena gracilis respirasome supercomplex I+III2+IV / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#89
• Source (natural): Organism: Euglena gracilis (euglena)

Macromolecule #1: NDUFS1a

• Macromolecule: Name: NDUFS1a / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 43.888836 KDa

Sequence

String:

MRRVLARFGP HVPRSFHTTV SRLQEAVPAS ILNAPVGLQP SQTVTCWIDH ILCEFQYPAD ITVFELARRN GINIPHFCYN RNLPIAGNC RMCMCHRVSD KKYAIACNEI AEPNAKYITV DDNLKNIRQY ILEFILANHS LDCPICDQGG ECDLQDLAEL Y GYDTSRYD YSDIKHEPDD MPINFLIKSD MNRCIHCTKC VRFLDNFSDD GKEGELGLMG RDPQTICVFR DDGNPQSYVA DI LSANVIE ICPVGALTGR ETNHETRPWE ITRLDAINIF DGTLSAINVE VKEGTELYRV NASKDPQNPD MLLNNEFITD RAR EAPQGN EFKRMTANYA ISLDNKKLLL HHALRLYAID PLFRSKALFL LADIMNEDRH LPETKQLGH

Macromolecule #2: NDUFS1b

• Macromolecule: Name: NDUFS1b / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 59.671816 KDa

Sequence

String:

MASGSEVLRQ FLTIRKNSYK YAPAFQRLHA LVNGANSAAK LRARHQKRLG INVVLGEKSD LGLCQLADTL ADRLKLADLG VSARPAKSP AVYYGHLAAQ QHRYAVPSEL KYTESSYSSR NVYIWLWTDV QQEAPDLHTQ IFTGPTSNCN VYSFGHVHNA R AGVKPVGG MEEFVGWLEG RTNLFSRTPK LETRLSNVYV LYSDNFLEMF PTNYGDIFKK IEELLGDQTF VSFSYLSRHP VS YNAVQTY AFPPVTQLLK RNDQYRLNVL TNVQRQDYSE NESRGRFTAR LMCHSTLLRA DQPMNELVIA QKTPAEDNAA LAY IDKFGD YKSAINSIFI SEFSDKLQLM HPHQLLTYAF ALLAWPRALA RLLPLTSIPK ADEEKTFKAT HSQFLERLIR DFDN DPTRL SLIHALSLGR PALVEDLRLR LWPYTVVPGT AFNVVKAKAL LQRLNATPEY SPDGPYYEFQ TPAAPVPSAA PTPAP QRVA LKSDSIFAID CEFVRHSMPL RGHINEVNRK QHLSWCKLAP ESK

Macromolecule #3: ND2b

• Macromolecule: Name: ND2b / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 15.756794 KDa

Sequence

String:

MNNNLQIENY TNKNKIVISP ISYIGNNHPY KMYTIINLCI SSSLLITNYT IAKTSIFLYL IYIFNNNIYF IIIMLFFVLY PIIFIVLIH PFIIISVNNH LINKANNKGI IINNFI(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)

Macromolecule #4: COXEG1

• Macromolecule: Name: COXEG1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 27.278852 KDa

Sequence

String:

MLSRALCSRN VPMSLKALNR PGSAGKLPMQ LMKYASAVAP ISHEGTLVRI SQVKKLSELQ LHFNDSHLGE SELAAKVLGK LRKLEAEVL ARNQAFNEAH PLVFDPKRAF NDEIFLCCSL CCIIFLIFLF NQYEEFAHEL SFDIREQFGL GFYMLLGLHG S HVIFGTIM LALLTLWGAQ GSVGPQSHAL RFTSLYVHLV DLVFIILVLA IYSANASPEL YGGIVPNILE ARTFVSVDAA GN PQIKEF

Macromolecule #5: COXEG3

• Macromolecule: Name: COXEG3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 16.394043 KDa

Sequence

String:

MMQRIPFKKP NQIRGYFTRV HKYNHVPVPF ILNVGMSISI VTSFVYFTYT SLWVRPEYDR VVDPSKAYVN PVWVDYWLKL RDEKRIQGA LERSILEEEP EKAAEKILEW ARTSAQNKIL EDLKLLKPAL SPATIAQFEK

Macromolecule #6: COXEG4

• Macromolecule: Name: COXEG4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 19.359492 KDa

Sequence

String:

MSTVLSILGK RFQRSALTPK MNPFIRIRCQ GPIEEFQRGF IGEFHAFALP GACMLVASCL GTFHIIRCLV VNPELSLAKV IPEILQPFT NPNAQLKAAD GKDDDDSQVP KQWGMWGRHP NYGVLHVPFL DALNKEALAR GKDGVNMGAE YNLVFTKSMA D QVVDLILD DVQKRV

Macromolecule #7: COXEG5

• Macromolecule: Name: COXEG5 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 19.464051 KDa

Sequence

String:

MPSSMAWTIG WGFYAAWIMK ETWNLRSSSV GWTPITLMEA YKTKERYLRS KAMMERYNSE LEAVDDSNIT EEDAKKFELE KATPSISIW EQFRSNPYWK EVEEEISTDV RKTMLEKHPD YALLLEAVKK SGYSKLWHLP GPWMNEHYND GLHGRFLGWT P KAAHH

Macromolecule #8: COXEG6

• Macromolecule: Name: COXEG6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 8.728172 KDa

Sequence

String:

VFPSITKPLG LFKNLPRQHR AARDASIWLA ILTAGPFGIF IAFKYYADWY DKKLLMEYYK DSIVYGETYG KGKYV

Macromolecule #9: COXEG7

• Macromolecule: Name: COXEG7 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 35.651516 KDa

Sequence

String:

MLRQVVRRSN PLRMQVRGSA WNFQELMESR IPDYKGRPNR SGAELEQVKA ALPKIEFMTS YEFDVLTKTR SNLTKEYSYQ RDMRLKVTE LMLDEAPHEL EGLAVEGDAA LKQLAELKAL QTLTEYAGDL LEGQNQIVQR VNDFVDSNPV YLLDQPLREE A RWNLLPEM DHKTRSLVRT ELRDWLPAEY RQTRAVDLQQ VAAFSPPVKA DMFRAIEARA KDAEAEIRSL PPAEQAGLLA LV KDNVAKS KAFIDPTYDI TPEAINACND VDALRAMAHR VTEYSGDARL LAIYGKAAQL TGDTAAQAIL KEAKDLVF

Macromolecule #10: COXEG8

• Macromolecule: Name: COXEG8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 24.49532 KDa

Sequence

String:

MLRVLTPAIA RPGLRCFFKD GFRDNASLEL VYRVVLKSPA VSQKLIEFYA KSLDQLSVES LSALKGTTVG IPLQPYLGDP HRVLLAYSL LPHTVETEAD GNPVVETKIG DEEQKIKIID SEVISFLAKE ILGKLGLETT PQAARQYLDS LVEGAEALYA K IAPVEPSP LEKAIAEINE EIKSGTPWDT LKNRADPKEL HALKFAQLPH PITKKVEGKF KYF

Macromolecule #11: COXEG9

• Macromolecule: Name: COXEG9 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 31.754857 KDa

Sequence

String:

MMNKGRILLG TNPGDIALNS KRFTVGKFVA WACGGWGLKD WIFPSLFIGR GDGPDFDRIV KHTLQSSSAI EKVNWFDSPF ACYTEWFVE HFPGFFDSRY RFEMSAKTIL ANKYPIKDFP VVDMRSWRSS RLFDLFEVPH PEHTFVFGGP VLLNTEAKRA E RLEQEWHG KDGTFVDVHP LNVATESHTE VSVIGGIKVY NGVWQGGKDS WKRDSAKPEL TAPFHSPIWY RNMFIVKNAD QL VEHFGEN LSDETWQEVR KEHLAFHERF HKDYSFA

Macromolecule #12: COXEG10

• Macromolecule: Name: COXEG10 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 10.043338 KDa

Sequence

String:

MNHERPWVFL NKVTGKWGAC GWQPFWKATS QSLNYIPDNF IALRTNGSWV RNLWQSSKVL DRGLNASGYP TVGTTDWVRI PLDAPLEA

Macromolecule #13: ND4L

• Macromolecule: Name: ND4L / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 19.673094 KDa

Sequence

String:

MLKAIQPMSL RSRVVSRALS SGYKQFGGLT FKEVQNNEVK QNPTTFMQRP LAQIASNGLF VAWPGQFETV FDLLTSQIGP YCVIGLYLG ARGCFKPEMA WTDRLIHVEA STFLLYGVFF ITFASTPLLY WAWFFMLFSN SLKTLMFVHL SNPWYLVLDQ P MQVKFSLK RLK

Macromolecule #14: COX5b-2

• Macromolecule: Name: COX5b-2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 19.736387 KDa

Sequence

String:

MFRRGLVLAA SRSKSLLDSV HVFRPEFAQG KFRIDLDQPA KQNKLQQQIY TLTDDEREMY EDEPYIGVDH LYEAHKGSKE NPVVVEAIG VHGNDVFTGC LGGCHKDAAD AVAYYTIVPP NTLAVCIDCG IHFVARINEQ LTFWPDGTQP WEKVDFKAVE G FLYKHYKY GSPLMI

Macromolecule #15: COX5c

• Macromolecule: Name: COX5c / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 23.788312 KDa

Sequence

String:

YFFSRARMSL QLGHMRVGAL WQSNANSRGL PNLLKEVMAA EPIYPYTHPG LSAKQILWNT KFNLKLEKDL ISIAESAVQS QTVNKVVIP TMLTGDVNPK SPASVVAKDK YQLAKQMAEF QYKKSYFEKP WGYMFAYYCD EDRARLCGGI GYVGHHGGGD A EILSYTEY VSTVVYPTIF YMAITFVVSL FLMYYYIYGT FFSSTRNRFD

Macromolecule #16: COX6a

• Macromolecule: Name: COX6a / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 13.079197 KDa

Sequence

String:

MSTNKNVFFP PALHLKENSI FQYKFKNLAV RHDAARLGII LAGPTLFYWF TVYYFKGMPN GLPPVLLNPF VNRNYRGQKR DMPWGTDCA FLDTKCHEEK DTWAKRGPFF IIA

Macromolecule #17: COX6b-1

• Macromolecule: Name: COX6b-1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 32.995148 KDa

Sequence

String:

MEGFVDKIDD NKYLGKWETI LTDGRTHLPK HITFHDAAAI SARWNQQYVN DSGPVYYRHW LACQQTYGAG NEDCRKLRWW AQQITHPLH LAEWDDWWKD EHYDLQIGQH WNRICGEEFE EASNLLKDLK EKREGLAAKF RDLLKTKTAE DPMGKILHEV A QLEEPSKT PVADLVEAGT LSKEAVEAAA ALKIKELKAL RDDATWAEVK GSLLNGVTTT CSTLKKTSKV VAELKAQAEL ER NKTSAVK LDIPHMRVNY EKPGLYEYDT WFGKFLPRTP QFGFAESDEE

Macromolecule #18: COX7a

• Macromolecule: Name: COX7a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 20.078975 KDa

Sequence

String:

MLSRLLHGPG SDKGHGYFHP DHGLVDSAAQ NIRGPYWHAE NMQFMQQTTQ SGEKLPVPLT ETAAQSSKLP VLSAQDGKTL QKIQLTDGS KVPKAATLVK WNPKSVHQWH SDDILRVDMT KHPEWVRTMR MFHGTVWRHR PEFRHPWFSR GSRASGIIML V FGAVAYGE ITFGKRLGGV

Macromolecule #19: COX7c

• Macromolecule: Name: COX7c / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 19.897588 KDa

Sequence

String:

MQKAVVGNLL RQLSLTRPRG HGSNLYNRVH GNLPQLYVEQ LYNTDEILDT VPHSSDPVHH MFPKCAAASP LGFRPYDNNK LWDAFVLWA IVYGVTTIFC VLHILKYPQI WKHLFETLTF QYTYQREIGE EYVWQYGGGG LNPAKWRFVK SPLEELGLVT E YRSDLDYP DDL

Macromolecule #20: NDUFA1

• Macromolecule: Name: NDUFA1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 15.659606 KDa

Sequence

String:

MPGGGGWSNM VPIIILNGVV WAALGRASLA CSPPEFHKRT KNDTEFNKYL HLRFNKAVQN PESVAGQAVK AGCAPEFRPF DSPANPLVV VYGWKDEIQP RPNPGSLAQS FDDRGLSWYQ SHFSNRVVDD PKHNSLPFPG YY

Macromolecule #21: NDUFA2

• Macromolecule: Name: NDUFA2 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 21.331168 KDa

Sequence

String:

MAQVWRSRLS CHFRKLRVRY PAAKLPEAAA INWATYLDVP SPANLPAADL NKALEAMRRP NPALASSRGV REFVQRVVPE LEAENPFCP LIVDKFDPEV ASQFPSESTD PTLHAHFLDG TQVNVPLANK SAAEIEDILA DLVKLAGLLQ PQAPLEGDNL P VEDTIYAA ASRPRFPNYS RHAKQARLGD ESTEM

Macromolecule #22: NDUFA3

• Macromolecule: Name: NDUFA3 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 14.950273 KDa

Sequence

String:

MDRYEFQKIR RQPPTLHWEA GNRFENIQRL RWENAALLKD PKLTWFRREM LMRPAFFHCT LFAGAVAVGY PFVAYFYEKV FPDRQDFRS TMTLLRAVGG LEEQEYYIME RAKAIERAKA RAAVQG

Macromolecule #23: NDUFA5

• Macromolecule: Name: NDUFA5 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 20.961854 KDa

Sequence

String:

MLRAGLRCAS AAAVIGRRGY ATKVKDFTGL AGYPVEPKWR GKLLALYAET LEAVQGIPAD HPYRRSVENL TRHYARVVEE AEDYEVVET TVGLGQVEQL IRMATNELQL VRDFTVWRTW EIDERVLDKM DVDLRTSIGG HYTKRAYEVL DEVEDERRLL E KLQQEVDL EKARVAAKAA EGPKTL

Macromolecule #24: NDUFA6

• Macromolecule: Name: NDUFA6 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 48.78698 KDa

Sequence

String:

MPQMRVATKG VRPFSVRAAG GQYVLPDHGR YGQVVRPARL EEFELNPHQN PSRDRDWSVE IRGFYRDLLK SIPTMKQRFR LVIPNDVVR QNIRKRFEQG PKLTDPAALR HRALMVSADL EEYFREDFLD SQVQGKYNNM DPRTLLNQEI AAAASETQTA H RFFNEGTN VLLETGIGGE DVTENRVYIT REQAYRKGLA SLRGDAAVRH LLPAVDPANQ TTLQALAAEN DLQALVDLLG HL PAAKTAE AYVQRCEAFH KEAGLRHQKA SGGAVLAAWE KFKDEEVNST VLLHPAYKAL IADPSRNPLL RGAADWVRLV EAG GLSTTE PDSAADKLLK VAQHLYYSDQ LPEGFAQDLG VSYLADLKGV DRRLDLLLDE EIAYRQELLL KIYAHTVESI KATA SNPTD PAAVKKHLDA HDWSAFVVPT EGVKSSYEAL AL

Macromolecule #25: NDUFA7

• Macromolecule: Name: NDUFA7 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 16.358675 KDa

Sequence

String:

MTTLKLNLKE RWLAMVHKRI QQWWQVGGDE RWVHTDPANY WWGAPAGLAS AREERQFFPS YYQRDLRRIK RKSFIMNRPM MKTNALALA DGQLPPRPHL NLADPSPFWW QSHPKDRGDR TMIYQSRSED AIEHYPR

Macromolecule #26: NDUFA8

• Macromolecule: Name: NDUFA8 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 25.916332 KDa

Sequence

String:

MTAPWNSSYT AKDWKKFPEI ESHAALQEKL DREVSSKDVV NVEKVVDDIL SHPHRKHFGT LNIPNEQTKK EHEGIFGMGI PENVLFSFG HWYRMSQPLE KAREEYLVCK HNAPKRAAPT ECLEEAKNMF NLYLHMSEIP FRTCPKQTAD YTYCVETQGH R KPGMDYGA RSFTSFVRYC LPEQEAFEEC LGKTYGCKFP PAPAHGQFYQ RSARFTNLPW DNIYA

Macromolecule #27: NDUFA9

• Macromolecule: Name: NDUFA9 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 54.983121 KDa

Sequence

String:

MARPPAEVTM LIKNAGDLLT KVKLENPPTR LLLDPKTIKL ATQDPTVKGK VKDLMLKGVK VEPSTAARVE HTFIPAPKQT ENQYSKPLL GYRLRELRTK VLSNEVYSTP RPRPLRGVVA TVFGGNGFLG NQVVAQLAQY GATVICPTRI NNEEHPVVMN T RDFRQIKS LGDQGQVFPV VYNPTVFDEV AQCVERSQVV FNCIGGFYPA MNQSQSFGPE ALFANLPRNI ARACAMKGVQ RL VHTSHIN ADVSSPIPFF KYKALGEEAV LDEFPNGIII RPADIFGDRD NFTTLMVNLL KGSNWPIMST NTYLLEGNEY VEC QPVWVV DVARAMVRAA MREYTFGQTY QLPGPDRYKL IEVMRYIEAI TQLQPSHVRV YSPLEAQLRF DRPGGENHRS WIDL HLREN VVPKPGVKTW QDLEIDNSIL TKMENITGDW MSKAPYRDMP TGFDEELTDL SLPRVWGDYD KKLIAFPAVS AVAAV LYAL AILFP

Macromolecule #28: NDUFAB1-alpha

• Macromolecule: Name: NDUFAB1-alpha / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 14.936051 KDa

Sequence

String:

MLARFGRVLS VAYRTYPVGK SLVRATPFIE AQKRWGGGHG HGPVTPLDSA IHFLDREDVT KRVLKIVAAF DKVTKEVKAD SHFVNDLGL DSLDVVEVVM SLEQEFNVDM SNQDAEKIHT VKDAIDYFCA HPASM

Macromolecule #29: NDUFAB1-beta

• Macromolecule: Name: NDUFAB1-beta / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 14.899051 KDa

Sequence

String:

MLARFGRVLS VAYRTYPVGK SLVRATPFIE AQKRWGGGHG HGPVTPLDSA IHFLDREDVT KRVLKIVAAF DKVTKEVKAD SHFVNDLGL DSLDVVEVVM ALEWEFQVEM HSSEAEMIAS VADAIDYFCL HPAAH

Macromolecule #30: NDUFA12

• Macromolecule: Name: NDUFA12 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 33.338133 KDa

Sequence

String:

MIRTLLLRHS APAVITGRPQ WWTQAIAVPP TQAEMELFQP KEVVHTKPYK PHPWFKDFGQ GRRHIVGPPE RGEFWRFRKF YAVMREKTK ELGVRGALRF LVRKLRTQRE AWYEKGYEED ILVGEDEMGN KYWQSSYTTA VQSRWVEYGT GSTFTKDASV V APEWYQWL HGAPDPEVQE LRPRHPAALT KGLTGDYWYR MKHSESQYAF GRKYWPRGNP HPKNTKYDDF LLRKRRLSKR RG FMEFDPF VLPAERLRKR AKWAPNPVSD RRHSAYSKNL PLGA

Macromolecule #31: NDUFA13

• Macromolecule: Name: NDUFA13 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 22.683824 KDa

Sequence

String:

MAEKAPEVHH PQDKLPEWFD PNSQQFTRNW RQAMPPKGGY PAVRHRYLSP GKGVSMRTAW AIIGSLTLFG LYQRRVDRAF ILDLGDDFH ARNCAAVPYM EAENQLKDLI SLHYEKEYRN FVAPGLKLDP SQFFNHPIPG AVGEYIRKTK PQRSTKNRVA W MNLELCTE PISQLSGAPQ SGPDKYERML GFTVYEGPAF

Macromolecule #32: NDUFA11

• Macromolecule: Name: NDUFA11 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 32.617457 KDa

Sequence

String:

MFQPVWQPIL MVGSPDIILH SAERRALAWD HPNRFSALRN ALYQARLLEQ PRPENRIALL GQDLLEDTIY TTVGAYLFAG VSCIQRLGG HVPFTPSFTG QNIWTMPKWA SRLLHQVRMM RYFSAYWAVG MTYFTTYNIL TGFMGFPVNE YHNYQPQASV L SVIPTALI YAALHPNRRP ERLWVGKATP FVGRFFLSGI VGAALAVFAA RRFAHATVSE LYHPSGSDSY FETLRNSAPS AD LVADMPY IPFYKEARCS PGLPVKSPYY DPEYVAKAKE EVKRKLDSLY

Macromolecule #33: NDUFB2

• Macromolecule: Name: NDUFB2 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 17.202416 KDa

Sequence

String:

RSSDLVRRGA LIASLVRNRT IRPKPLQVRT GSGGHHSHSV DPNDPFDGVE NELFLPPQAY YYFSRILFVT TLIVWLKTYR DKAIDQFSF KKWVDSLPEM TIKEEEIYLD EWEDWEAPQH DVARLFPRYT KKFRKADDWN FGATVW

Macromolecule #34: NDUFB3

• Macromolecule: Name: NDUFB3 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 6.831087 KDa

Sequence

String:

MVFWNHRRYQ FHPFINAWGK NMRHAFFPNL FWSR(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #35: NDUFB4

• Macromolecule: Name: NDUFB4 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 19.511402 KDa

Sequence

String:

MAERTFRSLG RLPAPSAPLP HDKIAWPEKI RFLPEGSIMM PDGYSLDPNQ HFEDPYHWSV LRRAWEPKQD VFVGKDSHGN VVKQYNSPQ VRHLHPGFKL FYLYRKGAAR RFQVNAQNIA YAAIFAGTCL ALCEFGLRVQ NWAGGVNTLE KLQSTERKVK M NGKVIVAP DSF

Macromolecule #36: NDUFB5

• Macromolecule: Name: NDUFB5 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 15.815024 KDa

Sequence

String:

FWQAEHKVYP PSKIHPGVHK GYIRTGVVHN LMRLLA(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)WAPVTALA SFATWR YRA EVAEWLDNLS NVDYPKWLAE REIAIRRVET EIFWRIEGHA QQRSQVASGQ GRAMLNKAAK ASYEESEASR LHGAGVS DL LK

Macromolecule #37: NDUFB6

• Macromolecule: Name: NDUFB6 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 10.926597 KDa

Sequence

String:

DIDLGPHPYR PDFAGHAPNP KHWRRNTFFL SCGILLIYYQ CCKLEFYHGR FLEPPIRPFP LQGYFRNVYL DDPNYDDKKK LMKEDFAKL KA

Macromolecule #38: NDUFB7

• Macromolecule: Name: NDUFB7 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 12.247089 KDa

Sequence

String:

EVFDNELVSY EGVDWEKIPN RFKRRRMPLS DEVMDEWKIP YRERDYCVHK LLELRKCVQK TFYRGECDHH RHEYHMCQHL ELRRRKAIK DLREELGR

Macromolecule #39: NDUFB8

• Macromolecule: Name: NDUFB8 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 20.488459 KDa

Sequence

String:

MLATRLRRPA VGLARTTIRM QARTRFMQME YWEVPMEADH PEYRPVVHDQ FLPFVGAPGV HWNPTPYPVG YDLWWNSFEA LAMDIHDMG HQESIGESLE KYLLGAGLCF AIFNFLILFD CHFWFSEVNA GHPLYPKQFH YLLRGGDPTD EIWLAQQQRE K ELMEHGKA LVKGEAKA

Macromolecule #40: NDUFB9

• Macromolecule: Name: NDUFB9 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 18.347 KDa

Sequence

String:

MKRSLLKLKD LKKVKVTQPQ SSIVLKPVPI GEPIIWSRVQ HLYWRALHVI EDFPWMGPSQ TSEQFDIMLS VKEEIQKNAN AAGALKDRL LEDFEAHIKR RDWMQRRVHY FANGGPGYQT FASERHTLEQ QGVWKAGTPI PFPKSDYNDD GPVEKGPYY

Macromolecule #41: NDUFB10

• Macromolecule: Name: NDUFB10 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 17.471033 KDa

Sequence

String:

MGQHFGKLGW DADDVYKVTT FEKHNYTVEE PRTPAFKAPR RPFPICDRPP MPDQEGLYET HADLYEMKRW VMWEWSVYCK ELSLVREEL KWCVAREGSN AHRECHDLAR QYTDMTRKLK MEERFSWLNH FKKPLVKDVL FDLKQ

Macromolecule #42: NDUFB11

• Macromolecule: Name: NDUFB11 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 13.048724 KDa

Sequence

String:

GGVSRPVRSK RETDFNSSDP WETPFGRIEH DDEDIPYTTT VYQPRRPTEW STDVIWHAVL LMFFVWFTTL QGILVPQNKE NDMAWEEAL RRRRLFREKQ V(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #43: Cytochrome c oxidase subunit 1

• Macromolecule: Name: Cytochrome c oxidase subunit 1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO / EC number: cytochrome-c oxidase
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 55.759504 KDa

Sequence

String:

MINNIMHMIN KYTLTTSHKI IGILYGYMGY IAGILGYIIS MLIRMELNTQ GLAIVRKVKE VTIYNNWITI HGLIMLFVFI MPVGIGFYG NYLIPMLIGT SELSMPRMNG ISFWMLIVGV VIFVISNVLM SKPISSGWTL YPPLSTRDAD NIGVNIDLSL L VVHVLGIS STIGSVNYIT TNKYNRHVGL TFMNINIYNF SIIVTSILLI GSLPILGVAI TGLLLDRNIN STIYDVIGDP VL YQHLFWF FGHPEVYVII LPVFGLTSLI LTSIIHKDIF GREGMMYCII SIGVVGYFVW AHHMFTVGLD IDSRSYFSIA TSI ISIPTS VKMFSYINTW ASGRGFRGNN SSWSFFSFLI CFCFGGFTGL LLSSGSLDIM LHDTYFVVGH FHTVLSLAAT FGLL IAHYF FLPIIFSYSI FESFSFYHTF LLLVGALLVF YPMHLAGLSG MARRVPEYAD IFIPFMTVGF HGTFLLIFST LTFIR SYFQ FLSHINHSNY L

UniProtKB: Cytochrome c oxidase subunit 1

Macromolecule #44: Cytochrome c oxidase subunit 2

• Macromolecule: Name: Cytochrome c oxidase subunit 2 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 22.443406 KDa

Sequence

String:

MRYGNREIES IILFFDQTII LYTSIINALI VGIIIIIRKW YNRKGICNQY IYHIKIEVIW TILPIIFLIV IVLHSVTVIY NLEINKGTN NKYINVIGNQ WYWIYNNIES RISSLGRIIL VDQPLFIKAN NNTHLIISSL DVIHSFALPT LGIKVDAIPG R INNISING LTQGLYVGYC SELCGSGHAF MPINLIVY

UniProtKB: Cytochrome c oxidase subunit 2

Macromolecule #45: Putative NADH dehydrogenase subunit 6

• Macromolecule: Name: Putative NADH dehydrogenase subunit 6 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 19.414947 KDa

Sequence

String:

MILNTNINYG GRNSRLCYLI PLMVHTIFLY LAYLIYYNIN LINIYLLLII IIFINIEWPL VIIYEYIYLT NYNIQNNLLY NIATFIFLE ILLFIGFYWL YINNIIHYPN NLPYNNNIAV NLLDHLNHNN NTDICLYIIL HNLLLLLYVT LILQLVHRYV Q L

UniProtKB: Putative NADH dehydrogenase subunit 6

Macromolecule #46: NDUFC2

• Macromolecule: Name: NDUFC2 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 22.010127 KDa

Sequence

String:

MDRSSVTPRQ VFLEPVELPF AKKDASLEIF TESQLKSLLE PADASLFKWK NLAKWKKEVR TLLPQEFQSW DPISNQETIV DLNPYLVGG IFGGFNAMAH NYLSCTVWWK VPWRFPMWMA AWAGVSAYLF EFRREVEYEN RKSRLLVNNY YNRVRTVIVE R EKRKNGPD HQFKDLPWMD REEQRSG

Macromolecule #47: COX4

• Macromolecule: Name: COX4 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 20.42748 KDa

Sequence

String:

MGGDAHAHGG HGLHADPIFN GSKVVRSIQM MRSYHRLPVG PEPPHLKIRG APLHPWSMYT DKGGFFFGVG TQLPRNFFPK FLATTSAIM GVTYGLVWLY NAAGPRAKTQ TRKWKELESE DPRFPFQEIP EIYLPSDIDR DPNADCWRIL NQVPRKEKLL V EITDPLRN FDFKVAKIKE N

Macromolecule #48: NDUEG1

• Macromolecule: Name: NDUEG1 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 53.351969 KDa

Sequence

String:

MRRVGVYALR CARGHASSAL AAAPASETLT VGSLYSKKLR EYPHKVLWRR KTGFENSSWN QCRRSHEALA TNFSEIEVGD RALFITDCD SLMVNVALAI QKRGAQVCIV PAQGLTSNKL ETYIEQLRPK VIFLGKDKVS VPDPLGGDEA PQKMTLDLYS M IWKIFPIS NVVEGLPLQS KRYPFVKSVV LCSQVDAINQ HDMITNIKYF ETPRDQDYYE SPLVHIAMHI APDFPALSVV DS AGKWTTH SHTTLLNAGR LFAAKYFGKA EHVLLLPGSE STPGGILTLY AALHAHAVLC YGEDQLVTKG DCKRVTRAVH QHE ATAVVG TKAQWDFLLK YGDAATKKED ATKEWFKSLK WAAVFLNDVE DAGDLASKIR SSFGIDRVEC IRGSPETLNL TSDG SGKLF DSLSVVIKDK DGVATRGDAE GFVWIKGPHV SAQYWNHIGL MSAPKDPNGY VKLPYVGKKK PDGGFTITTV QPVRE LVL

Macromolecule #49: NDUEG2

• Macromolecule: Name: NDUEG2 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 51.015891 KDa

Sequence

String:

MVIGTFFKTG FEKGLPLHEQ VVRHLLPLVP KARKGFWPYY FAVNERVVLP RRAGAALNSR LRIPGKNRRE CLPTSASSPL ELAQLRKAT DKPVEDVKPQ VFVSTSSPSD AVPLHNESVH SKWLEALDEV NKTASTFSDA FEIQNESLSK EIFHRLAVPA S LKAGNIFA HDGAFGSNSA DDIKFTAVTH DPTAALFLRH MVNPVPQVDP VDFPNLFSVF HIHDYEFTDP RIVEEFDGVK KE QLGITSP RFVLYDLAER NVYVSGSSQD LRDAIVCLGG LVAFHLYGSL TLACNSFIDK DGKLTLVFGS EANLNSPQLF GAH HSLWTP NGVSRAWNGV TVEGAKAQFA SDLVEVTAKG PRLTAPLPLQ LGGTARPRGA NLLAGAAAGT PEPPLAVDPK LPWR PNVVS AAGAKFVFVG KEEAKLSVDD AAALFADSHA AYPLGFSTKK KLAAKFKELA ATAPGASFVT TPNVSSL

Macromolecule #50: NDUEG3

• Macromolecule: Name: NDUEG3 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 63.09698 KDa

Sequence

String:

SRNVLTKGWR FHAHAGTFQS ALRFEEFQLK KSVGDVVLRL QCAPVTGFDL DQVRGLQGKV PLPAVGGLSG VGVVTEGSGI FKEGDRAVL LGANGAWSQY AVSSANHLLS VPATIPVEYA SLLASGPFAA YRILKAAHLK AGDLVLVNGA HTAIGLAALQ I MSRNVLTK GWRFHAHAGT FQSALRFEEF QLKKSVGDVV LRLQCAPVTG FDLDQVRGLQ GKVPLPAVGG LSGVGVVTEG SG IFKEGDR AVLLGANGAW SQYAVSSANH LLSVPATIPV EYASLLASGP FAAYRILKAA HLKAGDLVLV NGAHTAIGLA ALQ IAKAWG IDAVGVAHGA PALQVEKLKQ MGLNVVSSFA LDPKQVFGTS QPKFAISLVG GNAAAYVTHL IGSDGHIITC PLAS DEPHI LPNVDLVNKN LTIQTFSPWK SLLSATATEN EQMVSELCDL IAAHKLKANA VVRHEFGNLL DAIREAEHGT HNAVI LHEG TEKTWDNKNH DIYMEIDDKL QANWDAAAAA QDPYLKTGRD QPWQVLAEAE EVALPDELRV KLAAVTTEAE LLAVLD TLT LKERHLLGLP ATQAITVSAE ELKKMVSEFA S

Macromolecule #51: NDUEG4

• Macromolecule: Name: NDUEG4 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 41.313383 KDa

Sequence

String:

MQSLKRAGTL SRPFLRCFYD AGAPQIFRSN VPGRPLPWRQ ERQVPPNPSQ SKWQWEPEHI PTAEEYEAFP EVITLYGGDG LLRSSVIQE LVQSPRVSTI RVGTPWPDEF ASKLPGEWQS KVVAEFVDIL DRHSVLAAAE GSQALVNMMD IPYECELTYY Q AHVGSAQM ISHAANTCMC SRVIHVSSLA SRVDSWSRYS ESKFRGEDMS LACFPWTTIL RFGPLVGKNS PALKQFASYM KY APIYPCV AKDTKIQPTF VGDAAKAILA ALGNPSTRQL QFDLGGPEVF KHADFIKEVM RLTKASRPVV PVPGVIGDSI VAL LQWLPD PLVTRDMVYL IRSHHIANHD SMRTWKDLLP EHKLKTMAEA LQ

Macromolecule #52: NDUEG5

• Macromolecule: Name: NDUEG5 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 29.182729 KDa

Sequence

String:

MALAKGWRFS AHGGTWKAVL KLEDFPLTKT AGAAVLKVQA APVTPRDLDR IRGLYGALPL PAVAGTSGVG IVTQAGGAFK EGDRAVLAA ANPAGSYATL AAVDPAHLIK VPAALPVDVA ATLAVGPFAA YQILKLSGLK SGDSLALDGE ATLLGKSVAL L AKSRGITV VSGAAAGKDI KFALSLQGGR SASSLLGALG HGGQLLLHVA PSDEATVLDG ALVADKSVTI RSFAPAAVSA KE AEAMVEE VVELVKGNAL GLKVVRHDLA KLLEAVEEVT AGPSDTVHIL TLS

Macromolecule #53: NDUEG6

• Macromolecule: Name: NDUEG6 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 43.669457 KDa

Sequence

String:

MWRTSGCALR SRASFTTAVT AHASGVKAID PFKILSLPDS ATRDDLRNQF FELAKSNHPD VGGDKAKFQA IQDAYEDAIR IADQKHPVA PWDGISPMTY AQAWQGKDYW RKLWEEHWAA RLAHMYKHNA ELTTLEANKK WREAQYMQVK DWMVLAKDVL D PKTKAEWQ AGCELARDML LWTQANKKNY RRYFLSNQNV AVNMRQVYDE HEYWRQYENV QWAQWDAFFA RASAWALEHE EQ IRSVNST EGPLAAKFDY LFHGRLQYSS MSLEERLSRR AQEEKAYTRQ YWIAELMKAM RFSFRWVERF SRAFFPVLIL VVI AGYITD FQLIIRWLNI TRSETGALEV HNRKMDMVDW LLAGTPTPQN IEGTI

Macromolecule #54: NDUEG7

• Macromolecule: Name: NDUEG7 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 26.818773 KDa

Sequence

String:

QKLLPKISCK YLALFLGLAG ATTALGFGLA PPTELVQGKI KSAELQVGDL LTYKDGGHAK CHIKEHWAVY LGTGEEIAQL VGQDAAALK LQPEERYIGH RLANTHHEEP VVRIDPLSTA RPPQGGALYK AEAPALGEPE DREAIVKGIL ARRAEKDGSL A AKNCQHFS TWARYQYPYS NDSLETSSRF IVTRAGATAA AVFLGTLAVS QAPLFSTMFG AMGWMVGIFR PICNRQKVVT LQ KWEVEQ

Macromolecule #55: NDUEG8

• Macromolecule: Name: NDUEG8 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 23.994727 KDa

Sequence

String:

MTTHRPPLVT DRMFPWTWNS RYYMMTPLAT YGREDIFANP HLPADKKLHA AAVEGGEFMK DAYAPPHKAV SLVRRVGRKA MVGAGLYFH LFLMDKFVSK GLLDRNTSPY FYMTLYEKAI YCLLPNKNKP NEEDLLKLFR EARLESNYKS VSMWTYRLRS E RVPKSYFD KPPVSYDADR RTSGFVEWAF WMVGLESFGK SVVPEAW

Macromolecule #56: NDUEG9

• Macromolecule: Name: NDUEG9 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 18.272891 KDa

Sequence

String:

MSATFWKLTF GIPLSGDYDP TDIPNAEAEL LTHVIIKTIQ LCTVLGAVVA APVAAAAQPA ARTLAGLGAT AARYGKAGAL IGVPLGAFM HYARLQGITG IDPIYDRAYR LRHNANQHRA DRSFLRGAAL GAAVGYVTPI GVAGGYTAFS IAGLFLSGLY N TQLEAAAK AAEAAAAAAE

Macromolecule #57: NDUEG10

• Macromolecule: Name: NDUEG10 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 14.140555 KDa

Sequence

String:

MSKQNHGLHN RLIPIFVKEN AFNGFPLVNK GKWGSWLWFR NTQLKNLYHP LGAVLKWPRS FLMLNGTETA FWMVFFWAAA NTWLYSRQR KEFIKDCDYG (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #58: NDUEG11

• Macromolecule: Name: NDUEG11 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 11.472202 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)SL QCHDTRNRRD KCMVQHGFCA QQI EDHFEC LEQYYQKEKV DQLRREFRSQ SFQMVKQMKL EDLGHFDRYG NLR

Macromolecule #59: NDUEG11

• Macromolecule: Name: NDUEG11 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 11.323098 KDa

Sequence

String:

MHRIATRVAM QQRFYGVARP DVQNRVIQTV QPFVDICCRP RTEVKPTSHF VLDLNLPAVH LKQVVLALEG EFAAPVPDAQ FKNIVSVAD AVEYFAGHPQ AK

Macromolecule #60: NDUEG13

• Macromolecule: Name: NDUEG13 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 17.731408 KDa

Sequence

String:

MAHDKPHNEP PKRLLSKQEK TDMAVRENIE KPSWIKENGF QRWCYEMVRG VGRHPWPRYF ELTDFFGDKR HGDAIPRSWA AVKKLERGQ VIGPAVCVTL TLWVFVAGTI SKRNNTVWCD SIQEKYGFKQ PYSREFRTQH DGVLKPKLPA IY

Macromolecule #61: NDUFX

• Macromolecule: Name: NDUFX / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 37.196953 KDa

Sequence

String:

MTTIHAYTAT QKTVDGPSKK DWRGGRAAAI TMVFRPLFRS FVNPFSLARL MAFRPTSVLA LRTCQLRLGG GHGHGHGHGH GHGGHGGKP NKFENHIYRD ASGQPWFDLH KWELGRKPGK EDLEHPGRHE SFSQTKRLVS LTFIDKDGNP HNIKCEPGMN M ADAALQAG VSLDWLDGEG NQGFSAWSHM VISSPQFEML VPPDHREDKY LWDLEQWGTS HRNSRFVKFL FVQPWMEGMV VT HPFFADV VTPNFTDPFT GKIEDKDQAV APTYFVSKVV DPEKPDYKFP NPFELLWAGI DTYEQLWDYT RQSWLKRLEA RRQ KQQQA

Macromolecule #62: NDUCA1

• Macromolecule: Name: NDUCA1 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 48.726906 KDa

Sequence

String:

MAKLTWLKNL LNPYSRVPPS TFLRFFPRLL NKFGSAERDI NAEFPGTVHK HIKTYQERFM EQGAGDRIAT KWNPKPWEKA YMGQPDHPM TKAEQAKKED FMVGIHWDRS AGGRWTPNDK FPLFDYEFPI HPGRIILRWL YKQGKEPVNM QRSILVTDDF A TPSVYPFG WHAPSAILIG DACISNDAAV FDHCVLRADR AAIWVGPKSH VLEGCTLTTA PPTPDRPALG SVLIGENTVV GA GSSLNAC WIGDHCIIGS GCTIGFGARI DDGAVVGAGS VVEDDQYIPA GEVWVGRPAR YLRKTGDVDT FTAVAENDTL RSL HLAYSE YETTHGNVWA ESDKVCDNLE EEVAHRLQAH DVARAMVSKN FDAKLLKLPK SLVADLMDIV SDDDHPNPKP TVSA QARQH FSSQWDFNRK QEQRPVFTGN YNSPTMSRDM A

Macromolecule #63: NDUCA2

• Macromolecule: Name: NDUCA2 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 28.857973 KDa

Sequence

String:

MPLPQKSTLV RLGAAIRECG QALDRWGSFL QGRYGHLEKL QRTRRINGFH NFFPEVKGVR FIAPSASVIG QVTVSPGSSI WYNSVVRGD RGKVTIGEDT HILERVVIRS GILSVRDVKI GKDVIIEPGA IISPCQIEDG AYIGANAVLM EGCKIGKGVV V GPGAVVTE FAELTQPGVY QGVPAKSATA LTTEAAEAIT TRRAEFAKLA EEHEEMNTKL IEKQTEERVI LKDILENQLN EG NEFTMRS HHVARAPNVS PGNIAAGSAA

Macromolecule #64: NDUCA3

• Macromolecule: Name: NDUCA3 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 28.0169 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)GAA IRECGQALDR WGSFLQGRYG HLEKLQRTRR INGFHNFFPE VK GVRFIAP SASVIGQVTV SPGSSIWYNS VVRGDRGKVT IGEDTHILER VVIRSGILSV RDVKIGKDVI IEPGAIISPC QIE DGAYIG ANAVLMEGCK IGKGVVVGPG AVVTEFAELT QPGVYQGVPA KSATALTTEA AEAITTRRAE FAKLAEEHEE MNTK LIEKQ TEERVILKDI LEDQLNEGNE FTMRSHHVAR APNVSPGNIA AGSA

Macromolecule #65: ND1

• Macromolecule: Name: ND1 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 79.682836 KDa

Sequence

String:

MLNSNIYIII YGGIIMYSIM IIIQMFLYNF SNKIYIEVEI NKYILSKNNI DIYWIICNCT IIIIITTLNH IINKIGIYNM IEYNICYWL IGTGLGLYIS PFIVFGYKFF VYIMDLNNYS LNIYHNNNKM NDIQQIYNGT NYNDTMIFFI KDINNIFTIY R SINFFMNW LYQMIYYGVR MWLVFVLHSF SLGSFGELIT VITDNNLIFN VFYIGLLGLG VILYLIVIFY LGIQIYVYIS FS LSFLHST ILLFLVNYIP HYNNKSIFNT FTNKSIYLIP VSLVDLININ IIFYILLLYT LLLFFIPLFL ASINYTYHYI YKY YNYNYN FINNNTNTNN NNKFINISYN FFIILIILNI INISSIYNIL QILLIMLIVL SLSSLLTVLE RKGLASSQRR IGPS YNGWF GLVQIVQDGI KLIYKDYNRY NNINNKYIMI SCILNFIYSY LLFIFIYIDL ILYINISYII FMIIIILMIN HITII ICGI VINNSKWTIL SSIRLILLYF MYDIIFLLIL LYLSPINNLG INLLYNNNNL NLNNYIESQF YYINLYKYPL LLYIYI FIV LIEAGRIPVD LIESESELIS GYSIEYSGFL YALFASAEYS IILFHSILLS LLFFSYYSFN ILFIHITILF FIFVIIR ST LPRFKYTNLF NLTYYYILPF ILTYLLLL

Macromolecule #66: ND2a

• Macromolecule: Name: ND2a / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 35.864434 KDa

Sequence

String:

MLYNNNLIII ILMISIIIGI SLQNILVNDI SELRWINRFN LDNFIIIYII LLYNNIILIL GIISLIISTN KNTTNNKVQL IHMIIIIIN TIYICNNNNN TIINIILMII TIDILSVLNI ILIQKGEGIW YYFLYQSLMT ILIWWVLILD LSSLLSFFYY Y KLGSGIGG YYIPSLYSSI IYYNINLMIY IGTTNIILMY NPIFLFNNFN HNYFLIISNF LFILYILYIW IFNGYLFINL WL YSISFST IILANIYYLF TSIDFIYYNL FYYIYYFTIS SIIIWFIFIL SLYFINNYNN HIN

Macromolecule #67: ND3

• Macromolecule: Name: ND3 / type: protein_or_peptide / ID: 67 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 34.504223 KDa

Sequence

String:

MINNIIDIYI FNNNIINKSL INIIDIIILN IGILLILVEG SIRGIIGLII VGISINIYLI NNGYILISLL LIISIISISI IISLIKYNT MSIYKFNNNK YIQLFFILYL YIILINNNNY NNNINNIEII GYILIFESNI LLSLLILIMF MFIIILVKFN N KISINYNI IYFNVINYII LNIYILFIIA LLICYIPIII RYKSKIYKWS SDIYEVGIGV NMSNSIYNSI SNYNIGNYFI YF YIYLFIF LDIIINIIIL IYFININLFT YYFTLFFLIL AISYSLLFSY NFSNNF

Macromolecule #68: NADH-ubiquinone oxidoreductase chain 4

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 56.417312 KDa

Sequence

String:

MIYKIIILRY ISILIIMIIQ LMKMYNKEYR LISIISIISI LSIMNWPQSI KEINIYIINL IIGYEINLSY NYIGIYMSLI LDILIWISI LYERYYIKNN NTYNIIIYYI INNIFFSSND IITISLIYEF QTIPLLLIIN NSYNKINIYN KKGIGISNIL L ILYSIISG LLLYNSMYNI YLLNHTSNIS HNINILNYSY NHILINYILI NILISGSMKL SIFPFHIWLG KVHVEAPTIG SI LLAGISL KTGFYLHYLF IYLYQYIYIN YLIYFIYILF IGIIINNINI FYQIDTKRWI ALYSIIHMNL YYIIMLILLI SNN NNLYYI IFINILIYGM IGHSLISGGL FLIIGYIYDI TNNKNLYLIN NNIISSYIYF ILLLLLLANS SFPLFVLFIF ELLA FTSLS IFNILLSIFL LILSCSNLLS SLYIYYKYFY THYYSPIITF TSFDFILIIL AIPILIIIIL MGFNLLLPIH FLI

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

Macromolecule #69: ND5

• Macromolecule: Name: ND5 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 68.375359 KDa

Sequence

String:

MLIISIIKWL IISIIMNLSG NIIGINGLIY IIMLILVLSP IISWYVLYNI IIINIYNNKL IYLLSYNFIN YYNYNIDLEI GISIYEMIT VILLINVSYM INIYILKYLY KDKNVIRFVC IIMLFTYNMI LLIISNDLIM LFIGWEMIGI ISLLLINYYN N RIEATKAG LKAVVYNRIG DVFLLLSIIL SINMYNSNSI LLYNILISYM YYNINYININ LIIGMSFIIC AWSKSTQLGF QP WLLDAME GPTPVSALLH SATLVTAGII LLYKNRYILY YNSSLAILLL ILGGISCLLN SFSSINYLDI KRIVAYSTCT HIS LMIMIL GIDILINISE ISLLHLFYHG WSKSLIFMLC GYMISIIHSQ DLRFFGNLFQ HIPILFVIIN ISLLTILGFP GSYL SYSKD IILEFGLISI YGYNIILLFI IIILLSQGYS LGILLYLIYN YSYYNSTHNI YNFYSNKNNY IYIFAFLYLI IIIIY LPFL LYDILIYNNI SIMHHISYID PFSLIAFLGF ILSYYNYNYN HTLYIFNIHN NRLYIDKLLS SFMSIFSIHI IYYFQF ILE YGFIMHYLHI TNIIIFLIFL I

Macromolecule #70: MPP-beta

• Macromolecule: Name: MPP-beta / type: protein_or_peptide / ID: 70 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 53.520293 KDa

Sequence

String:

MTTPSLSSIL RHTRPIFKET LRAARPTLQN ALPNGFRIAS ESKDGDTCTV GVWIDAGSRW ETEKNNGVAH FLEHMNFKGT GKRSRQDIE FGMEKMGAHL NAYTSREHTC YYVKCFKKDV PEAVDILADI LLNSKRTEQD LDAERQTIVQ EKEDVEARID E VLMDHLHS AAFEGSGLGL SILGPLENIQ KSITKGMIDD FVKTHYTGPR MALVGSGAVD HGQLCDLASK YFGALPTGQP KP SGFTRFL GGDKRETNQL NPLTHVAVAF QTPGISHPDA IKIKVLEQLL GSYSRDKGEA AYSCFARAIV MDFYDPKVGQ FFR PNKAGH NPIHSLNAFW APYSDVGLLG FYAIAEPGKS YGHEWENILH YAMRELIRVS RNISEEEFER AKNQLKLQTM LQLD GTTNI ADDIGRQVLS FGARVPLASF FEQLDAISRE DLIRVAHEYF YDKDPVVAVI GDTDNVPEYD ALRAVTYSVD LGRA

Macromolecule #71: Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial

• Macromolecule: Name: Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial; type: protein_or_peptide / ID: 71 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 51.13266 KDa

Sequence

String:

MKSVVRSKGT QALFRRFSSA LGDSINPNQV GVGDNVIRVN GRLFEVDKVQ EKGLKTSVLD NGTKVITLDN GGSVAQLTFL YKDGPVYEN IFNAGISSFM KHALTKDGLT SSEYITKTFL QKAGIIVHEP TVVNKSAIAF TVEGFRDTLA QPAVADKFWQ S LLFPRFSP ENVKEVKRLV ELESKETKRD SPFAYLQDIL HKTAFKGSPL GHTSFVPAYN LGYIDSNKLF DRWDAHYGFG NI AVIATNI EHEAVLAAIT DSAWVARAHN KVGGVAAPAS KYSGGEGYDV VHRAKEFDDQ FTDVYSTYTA YAFKAPGRSN LKE HAASLV IAQALSNAVS PVLNTSFAPK RLEVFYQAYD TVGLIGLSSV QASNAQLKAF KAALSKIGTL SEADLAVHKS AALL TAYGN VESWRATQAT LIDSFNTTGQ PLSPLEIVSA IKAVSADTVK SVVATMLGSP ATLVHHGDSP CAPTLDALQ

UniProtKB: Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial

Macromolecule #72: Cytochrome b

• Macromolecule: Name: Cytochrome b / type: protein_or_peptide / ID: 72 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 42.476266 KDa

Sequence

String:

MNKISLYRSY STTILLSPAY SLGFCASIFI VIQIISGYIL ASNYIASTNE SFNIIHNVIM RELDTGWLIR FNHINGCAFL FIVIYMHIY RSLYHNSITK TSVWIVGIIM YILICGIAFT GYSLVYGQMS LWAIVVICSL VTAIPFIGNK LLILIWGGNI V SSVTLQRI FCIHYLLPLL LILFIIIHLY NLHNVNSTGD NYFINNRYDR INFYPLLLIR DVFIGSNILI IYNIFVYYYS DL FGHPDNY VPANPLVTPS EIMPEFYLLP FYALIRAIPH KVLGIIIMVL FLLSLTNLYP IYFIRFYNNI NILQRSLLLL LLL DLVIAS KLCLLINHYE SFYLLLILSI LCVLSHHIYN TSFNFSNSIS NL

UniProtKB: Cytochrome b

Macromolecule #73: Cytochrome c1, heme protein

• Macromolecule: Name: Cytochrome c1, heme protein / type: protein_or_peptide / ID: 73 / Number of copies: 2 / Enantiomer: LEVO / EC number: quinol-cytochrome-c reductase
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 27.889436 KDa

Sequence

String:

GVDSHPPALP WPHFQWFQGL DWRSVRRGKE VYEQVFAPCH SLSFIKYRHF EAFMSKEEVK NMAASFEVDD DPDEKGEARK RPGKRFDTV VQPYKNEQEA RYANNGALPP DLSVITNARH GGVDYIYALL TGYGRPVPGG VQLSTTQWYN PYFHGGIIGM P PPLTDDMI EYEDGTPASV PQMAKDVTCF LEWCSNPWWD ERKLLGYKTI ATLAVIAVSS GYYNRFLSGL WRSRRLAFRP FN YSK

UniProtKB: Cytochrome c1, heme protein

Macromolecule #74: UQCRFS1

• Macromolecule: Name: UQCRFS1 / type: protein_or_peptide / ID: 74 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 27.825922 KDa

Sequence

String:

MFKQLSRMYS TSPAVANAVG GLATSQIRDL VGNPAKSGKP VVKMPPYLKP SQLRPSRGAY SDEFVRENYI KAADPEDPYN YRSRAFTYA AKIPMWAGLL AGTRVTVVYL MSQFMPSKSS LALANIEVDI GDIPEGKTVT IMWQGKPVFL RKRTDAEIED M RAVPMDAL KDPQTDEERL GEGRWAVFMA VCTHLGCVPV IDQGSYNAYF CPCHGSHYDH SGRIRKGPAP LNLEVPPFKL LD DSTLFIG NADAA

Macromolecule #75: UQCRH

• Macromolecule: Name: UQCRH / type: protein_or_peptide / ID: 75 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 8.207571 KDa

Sequence

String:

GKMAAEQVSV SELKLKFEEE CAHHHCHDIV KKVEVCEESI KGKEGKNCLF QHARLWECVD HCASPKVFAL LK

Macromolecule #76: UQCRB

• Macromolecule: Name: UQCRB / type: protein_or_peptide / ID: 76 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 27.404115 KDa

Sequence

String:

MNDAPILAKL GLSKVNTYYF QRRKHIFALP ITIPNFPLAA LGIFRSTDMF EERTLERYSR DEREKTLGKA ALLLKEAKEK GNYSELVRL DPTDPRHPYY FEHPWQSALK MDTVSLTPYQ QYLRWHCLTY RAMHEWDRQG LLYDDLMQPK ALATDPFLEE A ILRLPYNQ RVERERRLSR AYDLALRREY LPDEDCIHPE DDVAYLHPYY HMVVDEHREQ HENPVDVYSR

Macromolecule #77: UQCRQ

• Macromolecule: Name: UQCRQ / type: protein_or_peptide / ID: 77 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 9.948402 KDa

Sequence

String:

EGHSAYVALE QRVNTRQKGI IQRHLSPYDV DIRGNGFRQI YRLGMQKIRY FGLIGTPCLL VAGAYRGIWV FCERLEDERY WSNAL

Macromolecule #78: UQCR9

• Macromolecule: Name: UQCR9 / type: protein_or_peptide / ID: 78 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 6.939355 KDa

Sequence

String:

MLANFSRFFY KTFLQSNATL IPLTLVFTY(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #79: UQCR10

• Macromolecule: Name: UQCR10 / type: protein_or_peptide / ID: 79 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 17.577297 KDa

Sequence

String:

MALEIKREWH VVPATKKFAD VPTEWPAKEH ERFALPYGPS EGRIVSFMRA SRIRNRLLFT LNPTGYTEDT YVTKEALRRQ ALAESKNLW YLPERPVVAD QLETIVNRST FFVVKGHKNK GLIGIAGARH NPLVWLPTLA LGGVWAWRWA SAGTI

Macromolecule #80: Ubiquinol-cytochrome-C reductase complex subunit IX, mitochondrial

• Macromolecule: Name: Ubiquinol-cytochrome-C reductase complex subunit IX, mitochondrial; type: protein_or_peptide / ID: 80 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 11.300364 KDa

Sequence

String:

MQTHVRRVAL QALRPCLRAG LMAPKFPVRF ATTAVSGELL TKTPYTRPGY AAQWTCLVVL FLKNQLLMRL FFAFVAYVVA MKVFGARFH VDHDEDATPA E

UniProtKB: Ubiquinol-cytochrome-C reductase complex subunit IX, mitochondrial

Macromolecule #81: NDUFS2

• Macromolecule: Name: NDUFS2 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 45.251949 KDa

Sequence

String:

MPARADARTM QHFTLNFGPA HPAAHGVLRL ILEMNHEYII RADPHIGFLH RGTEKLMEMK PVIQVTPYMD RLDYMSAMAN EHAFIMAVE KLCEIPVPLR GSYIRVIFLE ITRLLNHLLA VACAAADVGA LTPILWTFEE REKLMTFYEC VSGS(2MR)MH PN FFRPGGVASD LPYGFLDQLY EFINQFAARL DEVEDLLTQN NIWRERLKAG YMDLDRALSY GFSGPMLRAT GVAWDLRV T APYEVYPYLD VEVPYGVNSD SYDRYLIRMQ EMRNSIRIIH QCINDIVPGD YRLHDAKFAT IPFRDCKDSM ENLIAHFKY YSEGFRIPEG FVYCGTESPK GELGVYLQSN GDSLPYRVRI RAPGFYHLAA LPALAQDTMF PDLVTVIGTL DIVFGEVDR

Macromolecule #82: NDUFS3

• Macromolecule: Name: NDUFS3 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 32.466703 KDa

Sequence

String:

MFRRVATHVV PKLSTLLRAR NSIKFVTTQE VPPVHQGREV KSTPQNKGKD EFVRYLSSVL YPMASEIMWD DVTNDVTINV YPQYIRPVA KMLRDHQQFQ YKVLVDVTCV DTMTNLKRFD VVYNFLSVVF RHRVMVRASV DDASGIDSLT PLFHSAMWAE R EAWDMFGV FFVGHPDLRR MLSDYGFEGH PLRKDFPLGG YTEVYWDAPN STIKYASPPI FREEYRDYEN IETEWDDLYH RC NTAWAYD RMVKQGMESC DADYTYDDPA AHKKPQQTQG

Macromolecule #83: NDUFS4

• Macromolecule: Name: NDUFS4 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 23.736867 KDa

Sequence

String:

AGLAARPLHT SAPRLIFPFR RPFKSEPEDE TKIPDCYVNG KPIADGQGYQ AIGLAEHFER IRRNVNIRRR VPSRFTQMVG GRRARTFSI HFERGSVERE WNQGGRLGVE PFAMMRRKMK NFPTVEDAEA FALSAGWKIN SSNPGNMRFN PRVGGNRREF K QRYEFAKD SYMAKDSGKG AQYPQDPHSS GTRGTSSFQA KVTKRVVQQE

Macromolecule #84: NDUFS5

• Macromolecule: Name: NDUFS5 / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 14.244042 KDa

Sequence

String:

MDRYGKKDES TWWVLHPFHE KTYTEDLGKV STVEPSVCDE YIELFKRCAA KSLDPEAQCR SYQEDLKECR LGAKQRKFLS QYEFATNSF TVNEKNDFRN RWHETFFRGS LL(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)

Macromolecule #85: NDUFS6

• Macromolecule: Name: NDUFS6 / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 17.101748 KDa

Sequence

String:

MATTSRLSKV TVNIMPASRA LGVKPHRHRK QWQMPEVSFL KDVFSDPLMT EQKITERFIK AEFGPNRIDV RRAVPPIVVP KTFKHAWCD GNWGNRSTHG HPRISIRMKA GKVSQCKYCE NKFVRDDFPA FFAKREQVAA EEKYHDED

Macromolecule #86: NDUFS7

• Macromolecule: Name: NDUFS7 / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 22.837303 KDa

Sequence

String:

PQVKLLATWN PYNDPSLESV LQGDRKQTHM QRYAERQTLL GEELTPGQTH IRPGDNSVSW VSTTVSQVFK WGRSGSVWPL TFGLACCAI EMMQAYASRY DLDRYGTVPR GSPRQSDLII VAGTLTNKMA PCLRKVYDQM ATPCYVISMG SCANGGGYYH F SYAVVRGC NRVVPVDIYV PGCPPTAEAL VYGILLLQRK IKMG(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #87: NDUFS8

• Macromolecule: Name: NDUFS8 / type: protein_or_peptide / ID: 87 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 24.370025 KDa

Sequence

String:

MQHLKLARRG LPLQQLRSFH WTAPPLLRQP HPDVPKDVSG LGMGLAFMNQ FLLKEIMKAM WIVTIYTFRQ PSVTIHYPYE RNTKSTRFR GEHALLIYPD GDERCITCQL CEVTCPAQAI AIDGVEDDDG SRMASRFDLD MHKCIYCGLC QEACPVDAIV E TPHAEFCS TEYDSLLYDK ARLLENGIRW TSAIEYMVAK ERSDFPTNSQ RFKS

Macromolecule #88: NDUFV1

• Macromolecule: Name: NDUFV1 / type: protein_or_peptide / ID: 88 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 57.940191 KDa

Sequence

String:

MLLRGVRCGA LAGKQRRSII QVPDKAPKTP AGFLQDKDRI FQNIYNDHGQ GLKNAQKLGD WYRTKDLLAK GPEWIIDQIK KSGLRGRGG AGFPSGLKWS FMPKKPPADG RPSYVVINAD ESEPGTCKDR EIMRMEPHKL VEGTLIAGFA MRARAAYIYV R GEFYNETL SVEKAIKEAY DAGLLGDNAC GSGYKFDIVL CRGAGAYICG EETGLIESLE GKSGKPRLKP PFPANIGLWG CP TIVTNVE TVSVSPTILR RGPEWFASFG RKNNSGTKLF CISGHVNTPC TVEESMSIPL RELLEKHCGG VRGGWDNLLA VIP GGSSVP VLPKSICDDV LMDFDALMDE GSALGTAAVI VMDKSTDLIK ALARISMFYK NESCNQCTPC REGAGVMANI MDRI WKGNG FGPEVDMIKD IADNVELRSI CALGAAAAWP IQGLHKHFRE EMIQRIEDFT DISYNLHKDE MFGHGAAFDW RRTPK TFHG TLDSFQDSTY RPPAANNNAY RGQYPHVPIY EKEKSEADKI AA

Macromolecule #89: NDUFV2

• Macromolecule: Name: NDUFV2 / type: protein_or_peptide / ID: 89 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 25.189109 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)HIEWHN VRNAASDPFD FLPELYPEVY KILAKFPETK QQSAIIPLLH LAQRQNGGWV PLPG MLKIA EICEVPQKRV FECATFYCMF NHKPVGKYHI QVCVTTPCMI RGADEILHGL CHHLGVGEEE VTADGLFSIC EMECM GACV HAPMICISDY SNPPNYRYDY IEDLTLEQAI EVVDNLKKGI YPKVGSQQGR ASAIPLAGRT SLFDPPRGPF CRDLDK A

Macromolecule #90: poly(UNK)

• Macromolecule: Name: poly(UNK) / type: protein_or_peptide / ID: 90 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Euglena gracilis (euglena)
• Molecular weight: Theoretical: 1.039273 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #91: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 91 / Number of copies: 4 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

Macromolecule #92: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 92 / Number of copies: 6 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

Macromolecule #93: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 93 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #94: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

• Macromolecule: Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 94 / Number of copies: 41 / Formula: PC1
• Molecular weight: Theoretical: 790.145 Da

Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

Macromolecule #95: 1,2-Distearoyl-sn-glycerophosphoethanolamine

• Macromolecule: Name: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 95 / Number of copies: 9 / Formula: 3PE
• Molecular weight: Theoretical: 748.065 Da

Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM / Phosphatidylethanolamine

Macromolecule #96: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}p...

• Macromolecule: Name: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine; type: ligand / ID: 96 / Number of copies: 1 / Formula: S12
• Molecular weight: Theoretical: 523.597 Da

Chemical component information

ChemComp-S12:
O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine

Macromolecule #97: CARDIOLIPIN

• Macromolecule: Name: CARDIOLIPIN / type: ligand / ID: 97 / Number of copies: 31 / Formula: CDL
• Molecular weight: Theoretical: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

Macromolecule #98: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 98 / Number of copies: 3 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #99: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; type: ligand / ID: 99 / Number of copies: 1 / Formula: NDP
• Molecular weight: Theoretical: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

Macromolecule #100: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

• Macromolecule: Name: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate; type: ligand / ID: 100 / Number of copies: 2 / Formula: ZMP
• Molecular weight: Theoretical: 568.704 Da

Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

Macromolecule #101: HEME-A

• Macromolecule: Name: HEME-A / type: ligand / ID: 101 / Number of copies: 2 / Formula: HEA
• Molecular weight: Theoretical: 852.837 Da

Chemical component information

ChemComp-HEA:
HEME-A / Heme A

Macromolecule #102: COPPER (II) ION

• Macromolecule: Name: COPPER (II) ION / type: ligand / ID: 102 / Number of copies: 3 / Formula: CU
• Molecular weight: Theoretical: 63.546 Da

Chemical component information

ChemComp-CU:
COPPER (II) ION / Copper

Macromolecule #103: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 103 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #104: 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE

• Macromolecule: Name: 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE / type: ligand / ID: 104 / Number of copies: 3 / Formula: PX2
• Molecular weight: Theoretical: 535.671 Da

Chemical component information

ChemComp-PX2:
1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE

Macromolecule #105: UBIQUINONE-10

• Macromolecule: Name: UBIQUINONE-10 / type: ligand / ID: 105 / Number of copies: 1 / Formula: U10
• Molecular weight: Theoretical: 863.343 Da

Chemical component information

ChemComp-U10:
UBIQUINONE-10 / Coenzyme Q10

Macromolecule #106: PROTOPORPHYRIN IX CONTAINING FE

• Macromolecule: Name: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 106 / Number of copies: 4 / Formula: HEM
• Molecular weight: Theoretical: 616.487 Da

Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

Macromolecule #107: HEME C

• Macromolecule: Name: HEME C / type: ligand / ID: 107 / Number of copies: 2 / Formula: HEC
• Molecular weight: Theoretical: 618.503 Da

Chemical component information

ChemComp-HEC:
HEME C / Heme C

Macromolecule #108: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 108 / Number of copies: 1 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.3 mg/mL

Buffer

pH: 7.4
Component:

Concentration	Formula	Name
30.0 mM	Tris	Trimethylsilyl
100.0 mM	NaClSodium chloride	Sodium chloride
0.002 %	PMSF	Phenylmethylsulfonyl fluoridePMSF
0.1 %	GDN	Glyco-diosgenin
1.0 mM	EDTAEthylenediaminetetraacetic acid	Ethylene diamine tetraacetic acid

Details: SEC buffer (30 mM Tris pH 7.4, 100 mM NaCl, 0.002% PMSF, 0.1% GDN (w/v), 1mM EDTA)

• Grid: Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.039 kPa / Details: 15mA
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 105000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: FEI FALCON IV (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 9807 / Average exposure time: 7.8 sec. / Average electron dose: 51.5 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 3232325
• Startup model: Type of model: INSILICO MODEL; In silico model: 3D ab-inito model reconstruction in cryosparc
• Initial angle assignment: Type: COMMON LINE / Software - Name: cryoSPARC (ver. v3.3.2)
• Final 3D classification: Number classes: 4 / Avg.num./class: 98936 / Software - Name: cryoSPARC (ver. v3.3.2)
• Final angle assignment: Type: COMMON LINE / Software - Name: cryoSPARC (ver. v3.3.2)
• Final reconstruction: Number classes used: 1 / Applied symmetry - Point group: C₁ (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.81 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Version: v3.3.2 / Number images used: 345048

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: RIGID BODY FIT

Output model

PDB-8iuf:
Cryo-EM structure of Euglena gracilis super-complex I+III2+IV, composite