Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural basis of membrane skeleton organization in red blood cells.
Journal, issue, pages	Cell, Vol. 186, Issue 9, Page 1912-1929.e18, Year 2023
Publish date	Apr 27, 2023
Authors	Ningning Li / Siyi Chen / Kui Xu / Meng-Ting He / Meng-Qiu Dong / Qiangfeng Cliff Zhang / Ning Gao /
PubMed Abstract	The spectrin-based membrane skeleton is a ubiquitous membrane-associated two-dimensional cytoskeleton underneath the lipid membrane of metazoan cells. Mutations of skeleton proteins impair the ...The spectrin-based membrane skeleton is a ubiquitous membrane-associated two-dimensional cytoskeleton underneath the lipid membrane of metazoan cells. Mutations of skeleton proteins impair the mechanical strength and functions of the membrane, leading to several different types of human diseases. Here, we report the cryo-EM structures of the native spectrin-actin junctional complex (from porcine erythrocytes), which is a specialized short F-actin acting as the central organizational unit of the membrane skeleton. While an α-/β-adducin hetero-tetramer binds to the barbed end of F-actin as a flexible cap, tropomodulin and SH3BGRL2 together create an absolute cap at the pointed end. The junctional complex is strengthened by ring-like structures of dematin in the middle actin layers and by patterned periodic interactions with tropomyosin over its entire length. This work serves as a structural framework for understanding the assembly and dynamics of membrane skeleton and offers insights into mechanisms of various ubiquitous F-actin-binding factors in other F-actin systems.
External links	Cell / PubMed:37044097
Methods	EM (single particle)
Resolution	2.96 - 9.5 Å
Structure data	35301 8iah EMDB-35301, PDB-8iah: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Global map Method: EM (single particle) / Resolution: 3.6 Å 35302 8iai EMDB-35302, PDB-8iai: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Global map Method: EM (single particle) / Resolution: 3.5 Å EMDB-35303: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment Method: EM (single particle) / Resolution: 2.96 Å EMDB-35305: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Central segment Method: EM (single particle) / Resolution: 3.0 Å EMDB-35307: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment, TMCC1 optimized Method: EM (single particle) / Resolution: 3.2 Å EMDB-35308: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment, TMCC2 optimized Method: EM (single particle) / Resolution: 3.6 Å EMDB-35311: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Central segment, TMCC1 optimized Method: EM (single particle) / Resolution: 3.3 Å EMDB-35312: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Central segment, TMCC2 optimized Method: EM (single particle) / Resolution: 4.0 Å EMDB-35317: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment Method: EM (single particle) / Resolution: 3.6 Å EMDB-35318: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, adducin optimized Method: EM (single particle) / Resolution: 3.6 Å EMDB-35319: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, TMCC1 optimized Method: EM (single particle) / Resolution: 4.2 Å EMDB-35320: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, TMCC2 optimized Method: EM (single particle) / Resolution: 4.0 Å EMDB-35321: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, the second spectrin repeat dimer optimized Method: EM (single particle) / Resolution: 4.0 Å EMDB-35322: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, the first two spectrin repeat dimers optimized Method: EM (single particle) / Resolution: 4.4 Å EMDB-35324: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, the third to fifth spectrin repeat dimers optimized Method: EM (single particle) / Resolution: 9.5 Å EMDB-35325: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Barbed-end segment Method: EM (single particle) / Resolution: 3.7 Å EMDB-35327: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Barbed-end segment, adducin/TMCC1 optimized Method: EM (single particle) / Resolution: 3.8 Å 35329 8ib2 EMDB-35329, PDB-8ib2: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment, headpiece domain of dematin optimized Method: EM (single particle) / Resolution: 3.8 Å
Chemicals	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate
Source	sus scrofa (pig) pig (pig)
Keywords	MEMBRANE PROTEIN / Macrocomplex / membrane skeleton / spectrin-actin junction