EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of membrane skeleton organization in red blood cells.
ジャーナル・号・ページ	Cell, Vol. 186, Issue 9, Page 1912-1929.e18, Year 2023
掲載日	2023年4月27日
著者	Ningning Li / Siyi Chen / Kui Xu / Meng-Ting He / Meng-Qiu Dong / Qiangfeng Cliff Zhang / Ning Gao /
PubMed 要旨	The spectrin-based membrane skeleton is a ubiquitous membrane-associated two-dimensional cytoskeleton underneath the lipid membrane of metazoan cells. Mutations of skeleton proteins impair the ...The spectrin-based membrane skeleton is a ubiquitous membrane-associated two-dimensional cytoskeleton underneath the lipid membrane of metazoan cells. Mutations of skeleton proteins impair the mechanical strength and functions of the membrane, leading to several different types of human diseases. Here, we report the cryo-EM structures of the native spectrin-actin junctional complex (from porcine erythrocytes), which is a specialized short F-actin acting as the central organizational unit of the membrane skeleton. While an α-/β-adducin hetero-tetramer binds to the barbed end of F-actin as a flexible cap, tropomodulin and SH3BGRL2 together create an absolute cap at the pointed end. The junctional complex is strengthened by ring-like structures of dematin in the middle actin layers and by patterned periodic interactions with tropomyosin over its entire length. This work serves as a structural framework for understanding the assembly and dynamics of membrane skeleton and offers insights into mechanisms of various ubiquitous F-actin-binding factors in other F-actin systems.
リンク	Cell / PubMed:37044097
手法	EM (単粒子)
解像度	2.96 - 9.5 Å
構造データ	35301 8iah EMDB-35301, PDB-8iah: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Global map 手法: EM (単粒子) / 解像度: 3.6 Å 35302 8iai EMDB-35302, PDB-8iai: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Global map 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-35303: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment 手法: EM (単粒子) / 解像度: 2.96 Å EMDB-35305: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Central segment 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-35307: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment, TMCC1 optimized 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-35308: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment, TMCC2 optimized 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-35311: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Central segment, TMCC1 optimized 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-35312: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Central segment, TMCC2 optimized 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-35317: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-35318: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, adducin optimized 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-35319: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, TMCC1 optimized 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-35320: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, TMCC2 optimized 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-35321: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, the second spectrin repeat dimer optimized 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-35322: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, the first two spectrin repeat dimers optimized 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-35324: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, the third to fifth spectrin repeat dimers optimized 手法: EM (単粒子) / 解像度: 9.5 Å EMDB-35325: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Barbed-end segment 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-35327: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Barbed-end segment, adducin/TMCC1 optimized 手法: EM (単粒子) / 解像度: 3.8 Å 35329 8ib2 EMDB-35329, PDB-8ib2: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment, headpiece domain of dematin optimized 手法: EM (単粒子) / 解像度: 3.8 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM / アデノシン二リン酸
由来	sus scrofa (ブタ) pig (ブタ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Macrocomplex / membrane skeleton / spectrin-actin junction