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TitleStructural insights into the assembly and energy transfer of the Lhcb9-dependent photosystem I from moss Physcomitrium patens.
Journal, issue, pagesNat Plants, Vol. 9, Issue 8, Page 1347-1358, Year 2023
Publish dateJul 20, 2023
AuthorsHaiyu Sun / Hui Shang / Xiaowei Pan / Mei Li /
PubMed AbstractIn plants and green algae, light-harvesting complexes I and II (LHCI and LHCII) constitute the antennae of photosystem I (PSI), thus effectively increasing the cross-section of the PSI core. The moss ...In plants and green algae, light-harvesting complexes I and II (LHCI and LHCII) constitute the antennae of photosystem I (PSI), thus effectively increasing the cross-section of the PSI core. The moss Physcomitrium patens (P. patens) represents a well-studied primary land-dwelling photosynthetic autotroph branching from the common ancestor of green algae and land plants at the early stage of evolution. P. patens possesses at least three types of PSI with different antenna sizes. The largest PSI form (PpPSI-L) exhibits a unique organization found neither in flowering plants nor in algae. Its formation is mediated by the P. patens-specific LHC protein, Lhcb9. While previous studies have revealed the overall architecture of PpPSI-L, its assembly details and the relationship between different PpPSI types remain unclear. Here we report the high-resolution structure of PpPSI-L. We identified 14 PSI core subunits, one Lhcb9, one phosphorylated LHCII trimer and eight LHCI monomers arranged as two belts. Our structural analysis established the essential role of Lhcb9 and the phosphorylated LHCII in stabilizing the complex. In addition, our results suggest that PpPSI switches between different types, which share identical modules. This feature may contribute to the dynamic adjustment of the light-harvesting capability of PSI under different light conditions.
External linksNat Plants / PubMed:37474782
MethodsEM (single particle)
Resolution2.82 - 3.3 Å
Structure data

35018

8htu

EMDB-35018, PDB-8htu:
Cryo-EM structure of PpPSI-L
Method: EM (single particle) / Resolution: 2.87 Å

EMDB-35026: PpPSI-L overall map
Method: EM (single particle) / Resolution: 2.87 Å

EMDB-35027: Small Photosystem I moiety of Large Photosystem I from Physcomitrium patens
Method: EM (single particle) / Resolution: 2.82 Å

EMDB-35028: LHCII moiety of Large Photosystem I from Physcomitrium patens
Method: EM (single particle) / Resolution: 3.05 Å

EMDB-35033: OutLHCIs plus Lhcb9 moiety of Large Photosystem I from Physcomitrium patens
Method: EM (single particle) / Resolution: 3.29 Å

EMDB-35034: Outer LHCIs plus Lhcb9 moiety of Large Photosystem I from Physcomitrium patens processing with DeepEMhancer
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-CHL:
CHLOROPHYLL B / Chlorophyll b

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-LUT:
(3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Lutein

ChemComp-XAT:
(3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / Violaxanthin

ChemComp-NEX:
(1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-HOH:
WATER / Water

Source
  • physcomitrium patens (plant)
KeywordsPHOTOSYNTHESIS / Lhcb9 / PSI

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