EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into the assembly and energy transfer of the Lhcb9-dependent photosystem I from moss Physcomitrium patens.
ジャーナル・号・ページ	Nat Plants, Vol. 9, Issue 8, Page 1347-1358, Year 2023
掲載日	2023年7月20日
著者	Haiyu Sun / Hui Shang / Xiaowei Pan / Mei Li /
PubMed 要旨	In plants and green algae, light-harvesting complexes I and II (LHCI and LHCII) constitute the antennae of photosystem I (PSI), thus effectively increasing the cross-section of the PSI core. The moss ...In plants and green algae, light-harvesting complexes I and II (LHCI and LHCII) constitute the antennae of photosystem I (PSI), thus effectively increasing the cross-section of the PSI core. The moss Physcomitrium patens (P. patens) represents a well-studied primary land-dwelling photosynthetic autotroph branching from the common ancestor of green algae and land plants at the early stage of evolution. P. patens possesses at least three types of PSI with different antenna sizes. The largest PSI form (PpPSI-L) exhibits a unique organization found neither in flowering plants nor in algae. Its formation is mediated by the P. patens-specific LHC protein, Lhcb9. While previous studies have revealed the overall architecture of PpPSI-L, its assembly details and the relationship between different PpPSI types remain unclear. Here we report the high-resolution structure of PpPSI-L. We identified 14 PSI core subunits, one Lhcb9, one phosphorylated LHCII trimer and eight LHCI monomers arranged as two belts. Our structural analysis established the essential role of Lhcb9 and the phosphorylated LHCII in stabilizing the complex. In addition, our results suggest that PpPSI switches between different types, which share identical modules. This feature may contribute to the dynamic adjustment of the light-harvesting capability of PSI under different light conditions.
リンク	Nat Plants / PubMed:37474782
手法	EM (単粒子)
解像度	2.82 - 3.3 Å
構造データ	35018 8htu EMDB-35018, PDB-8htu: Cryo-EM structure of PpPSI-L 手法: EM (単粒子) / 解像度: 2.87 Å EMDB-35026: PpPSI-L overall map 手法: EM (単粒子) / 解像度: 2.87 Å EMDB-35027: Small Photosystem I moiety of Large Photosystem I from Physcomitrium patens 手法: EM (単粒子) / 解像度: 2.82 Å EMDB-35028: LHCII moiety of Large Photosystem I from Physcomitrium patens 手法: EM (単粒子) / 解像度: 3.05 Å EMDB-35033: OutLHCIs plus Lhcb9 moiety of Large Photosystem I from Physcomitrium patens 手法: EM (単粒子) / 解像度: 3.29 Å EMDB-35034: Outer LHCIs plus Lhcb9 moiety of Large Photosystem I from Physcomitrium patens processing with DeepEMhancer 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-CHL: CHLOROPHYLL B / Chlorophyll b ChemComp-CLA: CHLOROPHYLL A / クロロフィルa ChemComp-LUT: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / ルテイン ChemComp-XAT: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL / 13-cis-ビオラキサンチン / ビオラキサンチン ChemComp-NEX: (1R,3R)-6-{(3E,5E,7E,9E,11E,13E,15E,17E)-18-[(1S,4R,6R)-4-HYDROXY-2,2,6-TRIMETHYL-7-OXABICYCLO[4.1.0]HEPT-1-YL]-3,7,12,16-TETRAMETHYLOCTADECA-1,3,5,7,9,11,13,15,17-NONAENYLIDENE}-1,5,5-TRIMETHYLCYCLOHEXANE-1,3-DIOL ChemComp-LHG: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / ジパルミトイルホスファチジルグリセロ-ル / リン脂質YM / Phosphatidylglycerol ChemComp-BCR: BETA-CAROTENE / β-カロチン / Β-カロテン ChemComp-LMG: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE ChemComp-PQN: PHYLLOQUINONE / フィロキノン / フィロキノン ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-DGD: DIGALACTOSYL DIACYL GLYCEROL (DGDG) ChemComp-HOH: WATER / 水
由来	physcomitrium patens (ヒメツリガネゴケ)
キーワード	PHOTOSYNTHESIS (光合成) / Lhcb9 / PSI