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TitleStructures of human pannexin-1 in nanodiscs reveal gating mediated by dynamic movement of the N terminus and phospholipids.
Journal, issue, pagesSci Signal, Vol. 15, Issue 720, Page eabg6941, Year 2022
Publish dateFeb 8, 2022
AuthorsMaki Kuzuya / Hidemi Hirano / Kenichi Hayashida / Masakatsu Watanabe / Kazumi Kobayashi / Tohru Terada / Md Iqbal Mahmood / Florence Tama / Kazutoshi Tani / Yoshinori Fujiyoshi / Atsunori Oshima /
PubMed AbstractPannexin (PANX) family proteins form large-pore channels that mediate purinergic signaling. We analyzed the cryo-EM structures of human PANX1 in lipid nanodiscs to elucidate the gating mechanism and ...Pannexin (PANX) family proteins form large-pore channels that mediate purinergic signaling. We analyzed the cryo-EM structures of human PANX1 in lipid nanodiscs to elucidate the gating mechanism and its regulation by the amino terminus in phospholipids. The wild-type channel has an amino-terminal funnel in the pore, but in the presence of the inhibitor probenecid, a cytoplasmically oriented amino terminus and phospholipids obstruct the pore. Functional analysis using whole-cell patch-clamp and oocyte voltage clamp showed that PANX1 lacking the amino terminus did not open and had a dominant negative effect on channel activity, thus confirming that the amino-terminal domain played an essential role in channel opening. These observations suggest that dynamic conformational changes in the amino terminus of human PANX1 are associated with lipid movement in and out of the pore. Moreover, the data provide insight into the gating mechanism of PANX1 and, more broadly, other large-pore channels.
External linksSci Signal / PubMed:35133866
MethodsEM (single particle)
Resolution3.4 - 4.5 Å
Structure data

31489

7f8j

EMDB-31489, PDB-7f8j:
Cryo-EM structure of human pannexin-1 in a nanodisc
Method: EM (single particle) / Resolution: 3.6 Å

31490

7f8n

EMDB-31490, PDB-7f8n:
Human pannexin-1 showing a conformational change in the N-terminal domain and blocked pore
Method: EM (single particle) / Resolution: 3.4 Å

31491

7f8o

EMDB-31491, PDB-7f8o:
Cryo-EM structure of the C-terminal deletion mutant of human PANX1 in a nanodisc
Method: EM (single particle) / Resolution: 3.6 Å

32768

7wsv

EMDB-32768, PDB-7wsv:
Cryo-EM structure of the N-terminal deletion mutant of human pannexin-1 in a nanodisc
Method: EM (single particle) / Resolution: 4.5 Å

Chemicals

ChemComp-LBN:
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / phospholipid*YM / POPC

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / ATP release channel / vertebrate innexin homolog / MEMBRANE PROTEIN

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