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TitleCryo-EM structure of anchorless RML prion reveals variations in shared motifs between distinct strains.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 4005, Year 2022
Publish dateJul 13, 2022
AuthorsForrest Hoyt / Heidi G Standke / Efrosini Artikis / Cindi L Schwartz / Bryan Hansen / Kunpeng Li / Andrew G Hughson / Matteo Manca / Olivia R Thomas / Gregory J Raymond / Brent Race / Gerald S Baron / Byron Caughey / Allison Kraus /
PubMed AbstractLittle is known about the structural basis of prion strains. Here we provide a high (3.0 Å) resolution cryo-electron microscopy-based structure of infectious brain-derived fibrils of the mouse ...Little is known about the structural basis of prion strains. Here we provide a high (3.0 Å) resolution cryo-electron microscopy-based structure of infectious brain-derived fibrils of the mouse anchorless RML scrapie strain which, like the recently determined hamster 263K strain, has a parallel in-register β-sheet-based core. Several structural motifs are shared between these ex vivo prion strains, including an amino-proximal steric zipper and three β-arches. However, detailed comparisons reveal variations in these shared structural topologies and other features. Unlike 263K and wildtype RML prions, the anchorless RML prions lack glycophosphatidylinositol anchors and are severely deficient in N-linked glycans. Nonetheless, the similarity of our anchorless RML structure to one reported for wildtype RML prion fibrils in an accompanying paper indicates that these post-translational modifications do not substantially alter the amyloid core conformation. This work demonstrates both common and divergent structural features of prion strains at the near-atomic level.
External linksNat Commun / PubMed:35831291 / PubMed Central
MethodsEM (single particle)
Resolution3.0 Å
Structure data

25824

7td6

EMDB-25824, PDB-7td6:
aRML prion fibril
Method: EM (single particle) / Resolution: 3.0 Å

Source
  • mus musculus (house mouse)
  • house mouse (house mouse)
KeywordsPROTEIN FIBRIL / infectious prion / amyloid / parallel in-register

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