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TitleRecovery of particulate methane monooxygenase structure and activity in a lipid bilayer.
Journal, issue, pagesScience, Vol. 375, Issue 6586, Page 1287-1291, Year 2022
Publish dateMar 18, 2022
AuthorsChristopher W Koo / Frank J Tucci / Yuan He / Amy C Rosenzweig /
PubMed AbstractBacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using ...Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using inactive, detergent-solubilized pMMO lack several conserved regions neighboring the proposed active site. We show that reconstituting pMMO in nanodiscs with lipids extracted from the native organism restores methane oxidation activity. Multiple nanodisc-embedded pMMO structures determined by cryo-electron microscopy to 2.14- to 2.46-angstrom resolution reveal the structure of pMMO in a lipid environment. The resulting model includes stabilizing lipids, regions of the PmoA and PmoC subunits not observed in prior structures, and a previously undetected copper-binding site in the PmoC subunit with an adjacent hydrophobic cavity. These structures provide a revised framework for understanding and engineering pMMO function.
External linksScience / PubMed:35298269 / PubMed Central
MethodsEM (single particle)
Resolution2.14 - 3.65 Å
Structure data

EMDB-24826, PDB-7s4h:
CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.14 Angstrom resolution
Method: EM (single particle) / Resolution: 2.14 Å

EMDB-24827, PDB-7s4i:
CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.26 Angstrom resolution
Method: EM (single particle) / Resolution: 2.26 Å

EMDB-24828, PDB-7s4j:
CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.16 Angstrom resolution
Method: EM (single particle) / Resolution: 2.16 Å

EMDB-24829, PDB-7s4k:
CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.34 Angstrom resolution
Method: EM (single particle) / Resolution: 2.36 Å

EMDB-24830, PDB-7s4l:
CryoEM structure of Methylotuvimicrobium alcaliphilum 20Z pMMO in a POPC nanodisc at 2.46 Angstrom resolution
Method: EM (single particle) / Resolution: 2.46 Å

EMDB-24831, PDB-7s4m:
CryoEM structure of Methylocystis sp. str. Rockwell pMMO in a POPC nanodisc at 2.42 Angstrom resolution
Method: EM (single particle) / Resolution: 2.42 Å

EMDB-25683, PDB-7t4o:
CryoEM structure of Methylococcus capsulatus (Bath) pMMO treated with potassium cyanide in a native lipid nanodisc at 3.65 Angstrom resolution
Method: EM (single particle) / Resolution: 3.65 Å

EMDB-25684: CryoEM structure of Methylococcus capsulatus (Bath) pMMO treated with potassium cyanide and copper in a native lipid nanodisc at 3.62 angstrom resolution
PDB-7t4p: CryoEM structure of Methylococcus capsulatus (Bath) pMMO treated with potassium cyanide and copper in a native lipid nanodisc at 3.62 Angstrom resolution
Method: EM (single particle) / Resolution: 3.62 Å

Chemicals

ChemComp-CU:
COPPER (II) ION / Copper

ChemComp-D10:
DECANE / Decane

ChemComp-PLC:
DIUNDECYL PHOSPHATIDYL CHOLINE / phospholipid*YM

ChemComp-HXG:
1,2-dihexanoyl-sn-glycero-3-phosphocholine

ChemComp-P1O:
1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DDPC, phospholipid*YM

ChemComp-HOH:
WATER / Water

ChemComp-6ER:
(S)-2,3-bis(hexanoyloxy)propyl(2-(trimethylammonio)ethyl)phosphate

ChemComp-D12:
DODECANE / Dodecane

Source
  • Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) (bacteria)
  • methylococcus capsulatus str. bath (bacteria)
  • Methylotuvimicrobium alcaliphilum 20Z (bacteria)
  • methylomicrobium alcaliphilum (strain dsm 19304 / ncimb 14124 / vkm b-2133 / 20z) (bacteria)
  • methylocystis sp. atcc 49242 (bacteria)
KeywordsOXIDOREDUCTASE / Complex

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