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TitleCryo-Electron Tomography of Candida glabrata Plasma Membrane Proteins.
Journal, issue, pagesJ Fungi (Basel), Vol. 7, Issue 2, Year 2021
Publish dateFeb 6, 2021
AuthorsCristina Jiménez-Ortigosa / Jennifer Jiang / Muyuan Chen / Xuyuan Kuang / Kelley R Healey / Paul Castellano / Nikpreet Boparai / Steven J Ludtke / David S Perlin / Wei Dai /
PubMed AbstractFungal plasma membrane proteins have long been recognized as targets for the development of antifungal agents. Despite recent progress in experimental approaches and computational structural ...Fungal plasma membrane proteins have long been recognized as targets for the development of antifungal agents. Despite recent progress in experimental approaches and computational structural predictions, our knowledge of the structural dynamics and spatial distribution of these membrane proteins in the context of their native lipid environment remains limited. By applying cryo-electron tomography (cryoET) and subtomogram analysis, we aim to characterize the structural characteristics and spatial distribution of membrane proteins present in plasma membranes. This study has resulted in the identification of the membrane-embedded structure of the fungal H+-ATPase, Pma1. Tomograms of the plasma membrane revealed that Pma1 complexes are heterogeneously distributed as hexamers that cluster into distinct membrane microdomains. This study characterizes fungal membrane proteins in the native cellular landscape and highlights the unique potential of cryoET to advance our understanding of cellular biology and biological systems.
External linksJ Fungi (Basel) / PubMed:33562124 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution14.0 - 18.0 Å
Structure data

EMDB-23122:
Subtomogram average of the hexameric fungal plasma membrane proton pump Pma1 from protoplast membrane of Candida glabrata CBS138 strain
Method: EM (subtomogram averaging) / Resolution: 18.0 Å

EMDB-23123:
Subtomogram average of the hexameric fungal plasma membrane proton pump Pma1 from protoplast membrane of Candida glabrata KH238 strain
Method: EM (subtomogram averaging) / Resolution: 14.0 Å

Source
  • [Candida] glabrata (fungus)

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