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TitleVisualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.
Journal, issue, pagesNat Struct Mol Biol, Vol. 29, Issue 9, Page 942-953, Year 2022
Publish dateSep 12, 2022
AuthorsMichael Prattes / Irina Grishkovskaya / Victor-Valentin Hodirnau / Christina Hetzmannseder / Gertrude Zisser / Carolin Sailer / Vasileios Kargas / Mathias Loibl / Magdalena Gerhalter / Lisa Kofler / Alan J Warren / Florian Stengel / David Haselbach / Helmut Bergler /
PubMed AbstractThe AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from ...The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear export, a strict requirement for downstream maturation. The molecular mechanism of release remained elusive. Here, we report a series of cryo-EM structures that captured the extraction of Rlp24 from pre-60S particles by Saccharomyces cerevisiae Drg1. These structures reveal that Arx1 and the eukaryote-specific rRNA expansion segment ES27 form a joint docking platform that positions Drg1 for efficient extraction of Rlp24 from the pre-ribosome. The tips of the Drg1 N domains thereby guide the Rlp24 C terminus into the central pore of the Drg1 hexamer, enabling extraction by a hand-over-hand translocation mechanism. Our results uncover substrate recognition and processing by Drg1 step by step and provide a comprehensive mechanistic picture of the conserved modus operandi of AAA-ATPases.
External linksNat Struct Mol Biol / PubMed:36097293 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.8 Å
Structure data

14437

7z11

EMDB-14437, PDB-7z11:
Structure of substrate bound DRG1 (AFG2)
Method: EM (single particle) / Resolution: 3.2 Å

14471

7z34

EMDB-14471: Structure of pre-60S particle bound to DRG1(AFG2)
PDB-7z34: Structure of pre-60S particle bound to DRG1(AFG2).
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
  • saccharomyces cerevisiae s288c atcc 204508 / s288c (yeast)
KeywordsCHAPERONE / Ribosome biogenesis / AAA-ATPases / substrate recognition / single particle cryo-EM / RIBOSOME / large ribosomal subunit / substrate translocation mechanism

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