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Structure paper

TitleComplete atomic structure of a native archaeal cell surface.
Journal, issue, pagesCell Rep, Vol. 37, Issue 8, Page 110052, Year 2021
Publish dateNov 23, 2021
AuthorsAndriko von Kügelgen / Vikram Alva / Tanmay A M Bharat /
PubMed AbstractMany prokaryotic cells are covered by an ordered, proteinaceous, sheet-like structure called a surface layer (S-layer). S-layer proteins (SLPs) are usually the highest copy number macromolecules in ...Many prokaryotic cells are covered by an ordered, proteinaceous, sheet-like structure called a surface layer (S-layer). S-layer proteins (SLPs) are usually the highest copy number macromolecules in prokaryotes, playing critical roles in cellular physiology such as blocking predators, scaffolding membranes, and facilitating environmental interactions. Using electron cryomicroscopy of two-dimensional sheets, we report the atomic structure of the S-layer from the archaeal model organism Haloferax volcanii. This S-layer consists of a hexagonal array of tightly interacting immunoglobulin-like domains, which are also found in SLPs across several classes of archaea. Cellular tomography reveal that the S-layer is nearly continuous on the cell surface, completed by pentameric defects in the hexagonal lattice. We further report the atomic structure of the SLP pentamer, which shows markedly different relative arrangements of SLP domains needed to complete the S-layer. Our structural data provide a framework for understanding cell surfaces of archaea at the atomic level.
External linksCell Rep / PubMed:34818541 / PubMed Central
MethodsEM (subtomogram averaging) / EM (single particle)
Resolution3.46 - 15.87 Å
Structure data

13632

7ptp

EMDB-13632, PDB-7ptp:
In-situ structure of pentameric S-layer protein
Method: EM (subtomogram averaging) / Resolution: 11.58 Å

13634

7ptr

EMDB-13634, PDB-7ptr:
Structure of hexameric S-layer protein from Haloferax volcanii archaea
Method: EM (single particle) / Resolution: 3.46 Å

13637

7ptt

EMDB-13637, PDB-7ptt:
In-situ structure of hexameric S-layer protein
Method: EM (subtomogram averaging) / Resolution: 7.968 Å

13638

7ptu

EMDB-13638, PDB-7ptu:
Structure of pentameric S-layer protein from Halofaerax volcanii
Method: EM (single particle) / Resolution: 3.87 Å

EMDB-13639:
In-vitro structure of inverted S-layer tube
Method: EM (subtomogram averaging) / Resolution: 15.87 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-BGC:
beta-D-glucopyranose / Glucose

Source
  • haloferax volcanii ds2 (archaea)
  • Halobacterium volcanii (archaea)
  • haloferax volcanii (strain atcc 29605 / dsm 3757 / jcm 8879 / nbrc 14742 / ncimb 2012 / vkm b-1768 / ds2) (archaea)
KeywordsSTRUCTURAL PROTEIN / S-layer csg

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