EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of substrate progression through the bacterial chaperonin cycle.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 120, Issue 50, Page e2308933120, Year 2023
掲載日	2023年12月12日
著者	Scott Gardner / Michele C Darrow / Natalya Lukoyanova / Konstantinos Thalassinos / Helen R Saibil /
PubMed 要旨	The bacterial chaperonin GroEL-GroES promotes protein folding through ATP-regulated cycles of substrate protein binding, encapsulation, and release. Here, we have used cryoEM to determine structures ...The bacterial chaperonin GroEL-GroES promotes protein folding through ATP-regulated cycles of substrate protein binding, encapsulation, and release. Here, we have used cryoEM to determine structures of GroEL, GroEL-ADP·BeF, and GroEL-ADP·AlF-GroES all complexed with the model substrate Rubisco. Our structures provide a series of snapshots that show how the conformation and interactions of non-native Rubisco change as it proceeds through the GroEL-GroES reaction cycle. We observe specific charged and hydrophobic GroEL residues forming strong initial contacts with non-native Rubisco. Binding of ATP or ADP·BeF to GroEL-Rubisco results in the formation of an intermediate GroEL complex displaying striking asymmetry in the ATP/ADP·BeF-bound ring. In this ring, four GroEL subunits bind Rubisco and the other three are in the GroES-accepting conformation, suggesting how GroEL can recruit GroES without releasing bound substrate. Our cryoEM structures of stalled GroEL-ADP·AlF-Rubisco-GroES complexes show Rubisco folding intermediates interacting with GroEL-GroES via different sets of residues.
リンク	Proc Natl Acad Sci U S A / PubMed:38064510 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 4.4 Å
構造データ	15939 8ba7 EMDB-15939, PDB-8ba7: CryoEM structure of nucleotide-free GroEL-Rubisco. 手法: EM (単粒子) / 解像度: 4.4 Å 15940 8ba8 EMDB-15940, PDB-8ba8: CryoEM structure of GroEL-ADP.BeF3-Rubisco. 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-15941: CryoEM reconstruction of GroEL-ATP-Rubisco. 手法: EM (単粒子) / 解像度: 4.3 Å 15942 8ba9 EMDB-15942, PDB-8ba9: CryoEM structure of GroEL-GroES-ADP.AlF3-Rubisco. 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-15943: CryoEM reconstruction of GroEL-GroES-ADP.AlF3-Rubisco, class I. 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-15945: CryoEM reconstruction of GroEL-GroES-ADP.AlF3-Rubisco, class III. 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-15946: CryoEM reconstruction of GroEL-GroES-ADP.AlF3-Rubisco, class IV. 手法: EM (単粒子) / 解像度: 4.1 Å
化合物	ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-HOH: WATER / 水 ChemComp-AF3*: ALUMINUM FLUORIDE / トリフルオロアルミニウム / フッ化アルミニウム
由来	escherichia coli (大腸菌) escherichia coli k-12 (大腸菌)
キーワード	CHAPERONE (シャペロン) / GroEL / Rubisco (リブロース1,5-ビスリン酸カルボキシラーゼ/オキシゲナーゼ) / complex / nucleotide (ヌクレオチド) / Chameleon (カメレオン科) / GroES