EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	An ancestral interaction module promotes oligomerization in divergent mitochondrial ATP synthases.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 5989, Year 2022
掲載日	2022年10月11日
著者	Ondřej Gahura / Alexander Mühleip / Carolina Hierro-Yap / Brian Panicucci / Minal Jain / David Hollaus / Martina Slapničková / Alena Zíková / Alexey Amunts /
PubMed 要旨	Mitochondrial ATP synthase forms stable dimers arranged into oligomeric assemblies that generate the inner-membrane curvature essential for efficient energy conversion. Here, we report cryo-EM ...Mitochondrial ATP synthase forms stable dimers arranged into oligomeric assemblies that generate the inner-membrane curvature essential for efficient energy conversion. Here, we report cryo-EM structures of the intact ATP synthase dimer from Trypanosoma brucei in ten different rotational states. The model consists of 25 subunits, including nine lineage-specific, as well as 36 lipids. The rotary mechanism is influenced by the divergent peripheral stalk, conferring a greater conformational flexibility. Proton transfer in the lumenal half-channel occurs via a chain of five ordered water molecules. The dimerization interface is formed by subunit-g that is critical for interactions but not for the catalytic activity. Although overall dimer architecture varies among eukaryotes, we find that subunit-g together with subunit-e form an ancestral oligomerization motif, which is shared between the trypanosomal and mammalian lineages. Therefore, our data defines the subunit-g/e module as a structural component determining ATP synthase oligomeric assemblies.
リンク	Nat Commun / PubMed:36220811 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 4.3 Å
構造データ	15559 8ap6 EMDB-15559, PDB-8ap6: Trypanosoma brucei mitochondrial F1Fo ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.2 Å 15560 8ap7 EMDB-15560, PDB-8ap7: membrane region of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 2.7 Å 15561 8ap8 EMDB-15561, PDB-8ap8: Peripheral stalk of Trypanosoma brucei mitochondrial ATP synthase 手法: EM (単粒子) / 解像度: 3.7 Å 15562 8ap9 EMDB-15562, PDB-8ap9: rotor of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.7 Å 15563 8apa EMDB-15563, PDB-8apa: rotational state 1a of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.7 Å 15564 8apb EMDB-15564, PDB-8apb: rotational state 1b of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.8 Å 15565 8apc EMDB-15565, PDB-8apc: rotational state 1c of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.5 Å 15566 8apd EMDB-15566, PDB-8apd: rotational state 1d of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.7 Å 15567 8ape EMDB-15567, PDB-8ape: rotational state 1e of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.7 Å 15568 8apf EMDB-15568, PDB-8apf: rotational state 2a of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 4.3 Å 15570 8apg EMDB-15570, PDB-8apg: rotational state 2b of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.5 Å 15571 8aph EMDB-15571, PDB-8aph: rotational state 2c of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.8 Å 15572 8apj EMDB-15572, PDB-8apj: rotational state 2d of Trypanosoma brucei mitochondrial ATP synthase 手法: EM (単粒子) / 解像度: 3.8 Å 15573 8apk EMDB-15573, PDB-8apk: rotational state 3 of the Trypanosoma brucei mitochondrial ATP synthase dimer 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-Q7G: 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, リン脂質YM / Discrete optimized protein energy ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-UTP: URIDINE 5'-TRIPHOSPHATE / UTP / UTPYM / ウリジン三リン酸 ChemComp-HOH: WATER / 水 ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM / ホスファチジルエタノールアミン
由来	trypanosoma brucei brucei (ブルーストリパノソーマ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / ATP synthase (ATP合成酵素) / mitochondria (ミトコンドリア)