EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture of the human erythrocyte ankyrin-1 complex.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 29, Issue 7, Page 706-718, Year 2022
掲載日	2022年7月14日
著者	Francesca Vallese / Kookjoo Kim / Laura Y Yen / Jake D Johnston / Alex J Noble / Tito Calì / Oliver Biggs Clarke /
PubMed 要旨	The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex, but how it tethers the spectrin-actin cytoskeleton to the lipid bilayer and the nature of its association ...The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex, but how it tethers the spectrin-actin cytoskeleton to the lipid bilayer and the nature of its association with the band 3 anion exchanger and the Rhesus glycoproteins remains unknown. Here we present structures of ankyrin-1 complexes purified from human erythrocytes. We reveal the architecture of a core complex of ankyrin-1, the Rhesus proteins RhAG and RhCE, the band 3 anion exchanger, protein 4.2, glycophorin A and glycophorin B. The distinct T-shaped conformation of membrane-bound ankyrin-1 facilitates recognition of RhCE and, unexpectedly, the water channel aquaporin-1. Together, our results uncover the molecular details of ankyrin-1 association with the erythrocyte membrane, and illustrate the mechanism of ankyrin-mediated membrane protein clustering.
リンク	Nat Struct Mol Biol / PubMed:35835865 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	2.17 - 25.0 Å
構造データ	26874 7uz3 EMDB-26874, PDB-7uz3: Band 3-Glycophorin A complex, outward facing 手法: EM (単粒子) / 解像度: 2.35 Å 26886 7uze EMDB-26886, PDB-7uze: Erythrocyte ankyrin-1 complex class 2 local refinement of AQP1 (C4 symmetry applied) 手法: EM (単粒子) / 解像度: 2.4 Å 26916 7uzq EMDB-26916, PDB-7uzq: Local refinement of RhAG-RhCE-ANK1(AR1-5), from consensus refinement of all classes 手法: EM (単粒子) / 解像度: 2.17 Å 26917 7uzs EMDB-26917, PDB-7uzs: Protein 4.2 (local refinement from consensus reconstruction of ankyrin complex classes) 手法: EM (単粒子) / 解像度: 2.2 Å 26918 7uzu EMDB-26918, PDB-7uzu: Ankyrin-1 (N-terminal region of membrane binding domain, local refinement from consensus reconstruction; bound to N-terminal peptide from band 3) 手法: EM (単粒子) / 解像度: 2.3 Å 26919 7uzv EMDB-26919, PDB-7uzv: Cytoplasmic domains of Band 3-I (local refinement from consensus reconstruction of ankyrin complexes) 手法: EM (単粒子) / 解像度: 2.5 Å 26940 7v07 EMDB-26940, PDB-7v07: Band 3-I-TM local refinement from erythrocyte ankyrin-1 complex consensus reconstruction 手法: EM (単粒子) / 解像度: 2.8 Å 26943 7v0k EMDB-26943, PDB-7v0k: Consensus refinement of human erythrocyte ankyrin-1 complex (Composite map) 手法: EM (単粒子) / 解像度: 2.4 Å 26944 7v0m EMDB-26944, PDB-7v0m: Local refinement of ankyrin-1 (N-terminal half), class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.7 Å 26948 7v0q EMDB-26948, PDB-7v0q: Local refinement of protein 4.2, class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.5 Å 26949 7v0s EMDB-26949, PDB-7v0s: Local refinement of RhAG/CE trimer, class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.5 Å 26950 7v0t EMDB-26950, PDB-7v0t: Local refinement of Band 3-I cytoplasmic domains, class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.7 Å 26951 7v0u EMDB-26951, PDB-7v0u: Local refinement of Band 3-II cytoplasmic domains, class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.0 Å 26952 7v0x EMDB-26952, PDB-7v0x: Local refinement of ankyrin-1 (C-terminal half), class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.0 Å 26953 7v0y EMDB-26953, PDB-7v0y: Local refinement of Band 3-III cytoplasmic domains, class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.0 Å 26954 7v19 EMDB-26954, PDB-7v19: Local refinement of Band 3-II transmembrane domains, class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.3 Å 26955 8crq EMDB-26955, PDB-8crq: Local refinement of Band 3-I transmembrane domains, class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.2 Å 26956 8crr EMDB-26956, PDB-8crr: Local refinement of Band 3-III transmembrane domains, class 1 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.0 Å 26958 8crt EMDB-26958, PDB-8crt: Local refinement of Rh trimer, glycophorin B and Band3-III transmembrane region, class 1a of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.0 Å 26960 8cs9 EMDB-26960, PDB-8cs9: Composite reconstruction of Class 1 of the erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.74 Å 26965 8csl EMDB-26965, PDB-8csl: Sub-tomogram averaging of erythrocyte ankyrin-1 complex 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å 26972 8csv EMDB-26972, PDB-8csv: Local refinement of Anykyrin-1 (N-terminal half of membrane binding domain) in Class 2 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.7 Å 26973 8csw EMDB-26973, PDB-8csw: Local refinement of protein 4.2 in Class 2 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.5 Å 26974 8csx EMDB-26974, PDB-8csx: Local refinement of RhAG/CE trimer in class 2 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.4 Å 26975 8csy EMDB-26975, PDB-8csy: Local refinement of cytoplasmic domains of band3-I in class 2 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 2.7 Å 26978 8ct2 EMDB-26978, PDB-8ct2: Local refinement of AQP1 tetramer (C1; refinement mask included D1 of protein 4.2 and Ankyrin-1 AR1-5) in Class 2 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.1 Å 26979 8ct3 EMDB-26979, PDB-8ct3: Local refinement of band3-I transmembrane region from class 2 of erythrocyte ankyrin-1 complex 手法: EM (単粒子) / 解像度: 3.3 Å 26988 8cte EMDB-26988, PDB-8cte: Class 2 of erythrocyte ankyrin-1 complex (Composite map) 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-CLR: CHOLESTEROL / コレステロ-ル / コレステロール ChemComp-PLC: DIUNDECYL PHOSPHATIDYL CHOLINE / O-(1-O,2-O-ジドデカノイル-L-グリセロ-3-ホスホ)コリン / リン脂質YM ChemComp-PIO: [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate / 1-O-(1-O,2-O-ジオクタノイル-L-グリセロ-3-ホスホ)-D-myo-イノシト-ル4,5-ビスりん酸 ChemComp-HOH: WATER / 水 ChemComp-AJP: Digitonin / ジギトニン / 可溶化剤YM / ジギトニン ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	homo sapiens (ヒト) human (ヒト)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / Membrane Protein (膜タンパク質) / Anion Exchange (イオン交換) / Erythrocyte (赤血球) / Glycoprotein (糖タンパク質) / STRUCTURAL PROTEIN (タンパク質) / Ankyrin (アンキリン) / TRANSPORT PROTEIN/STRUCTURAL PROTEIN / TRANSPORT PROTEIN-STRUCTURAL PROTEIN complex / ankyrin complex (アンキリン)