EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Flagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 1422, Year 2022
掲載日	2022年3月17日
著者	Mark A B Kreutzberger / Richard C Sobe / Amber B Sauder / Sharanya Chatterjee / Alejandro Peña / Fengbin Wang / Jorge A Giron / Volker Kiessling / Tiago R D Costa / Vincent P Conticello / Gad Frankel / Melissa M Kendall / Birgit E Scharf / Edward H Egelman /
PubMed 要旨	Flagellar filaments function as the propellers of the bacterial flagellum and their supercoiling is key to motility. The outer domains on the surface of the filament are non-critical for motility in ...Flagellar filaments function as the propellers of the bacterial flagellum and their supercoiling is key to motility. The outer domains on the surface of the filament are non-critical for motility in many bacteria and their structures and functions are not conserved. Here, we show the atomic cryo-electron microscopy structures for flagellar filaments from enterohemorrhagic Escherichia coli O157:H7, enteropathogenic E. coli O127:H6, Achromobacter, and Sinorhizobium meliloti, where the outer domains dimerize or tetramerize to form either a sheath or a screw-like surface. These dimers are formed by 180° rotations of half of the outer domains. The outer domain sheath (ODS) plays a role in bacterial motility by stabilizing an intermediate waveform and prolonging the tumbling of E. coli cells. Bacteria with these ODS and screw-like flagellar filaments are commonly found in soil and human intestinal environments of relatively high viscosity suggesting a role for the dimerization in these environments.
リンク	Nat Commun / PubMed:35301306 / PubMed Central
手法	EM (らせん対称) / EM (単粒子)
解像度	3.5 - 7.7 Å
構造データ	25211 7sn4 EMDB-25211, PDB-7sn4: Cryo-EM structure of the enterohemorrhagic E. coli O157:H7 flagellar filament 手法: EM (らせん対称) / 解像度: 3.6 Å EMDB-25212: Cryo-EM structure of the N319F, N323F EHEC O157:H7 flagellar filament 手法: EM (らせん対称) / 解像度: 7.7 Å 25213 7sn7 EMDB-25213, PDB-7sn7: Cryo-EM structure of the enteropathogenic E. coli O127:H6 flagellar filament 手法: EM (単粒子) / 解像度: 4.2 Å 25215 7sn9 EMDB-25215, PDB-7sn9: Cryo-EM structure of the Sinorhizobium meliloti flagellar filament 手法: EM (らせん対称) / 解像度: 3.5 Å 25382 7sqd EMDB-25382, PDB-7sqd: Cryo-EM structure of the Achromobacter flagellar filament 手法: EM (らせん対称) / 解像度: 3.7 Å 25386 7sqj EMDB-25386, PDB-7sqj: Cryo-EM structure of the seam subunits of the enteropathogenic E. coli O127:H6 flagellar filament 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-25388: Cryo-EM map of the EHEC O157:H7 flagellar filament outer domain sheath with D1 symmetry 手法: EM (らせん対称) / 解像度: 3.6 Å
由来	Escherichia coli (大腸菌) escherichia coli o157:h7 (大腸菌) escherichia coli o127:h6 (大腸菌) sinorhizobium meliloti (根粒菌) achromobacter sp. (バクテリア)
キーワード	STRUCTURAL PROTEIN (タンパク質) / Bacteria flagellar filament / motility (運動性) / flagellar polymorphism / Bacterial flagella (鞭毛) / soil