EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites.
ジャーナル・号・ページ	Mol Cell, Vol. 81, Issue 23, Page 4891-44906.e8, Year 2021
掲載日	2021年12月2日
著者	Frank Bürmann / Louise F H Funke / Jason W Chin / Jan Löwe /
PubMed 要旨	The ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion. MukBEF ...The ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion. MukBEF activity within the replication terminus macrodomain is suppressed by the sequence-specific unloader MatP. Here, we present the complete atomic structure of MukBEF in complex with MatP and DNA as determined by electron cryomicroscopy (cryo-EM). The complex binds two distinct DNA double helices corresponding to the arms of a plectonemic loop. MatP-bound DNA threads through the MukBEF ring, while the second DNA is clamped by the kleisin MukF, MukE, and the MukB ATPase heads. Combinatorial cysteine cross-linking confirms this topology of DNA loop entrapment in vivo. Our findings illuminate how a class of near-ubiquitous DNA organizers with important roles in genome maintenance interacts with the bacterial chromosome.
リンク	Mol Cell / PubMed:34739874 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 13.0 Å
構造データ	12656 7nyw EMDB-12656, PDB-7nyw: Cryo-EM structure of the MukBEF-MatP-DNA head module 手法: EM (単粒子) / 解像度: 3.1 Å 12657 7nyx EMDB-12657, PDB-7nyx: Cryo-EM structure of the MukBEF-MatP-DNA monomer (closed conformation) 手法: EM (単粒子) / 解像度: 4.6 Å 12658 7nyy EMDB-12658, PDB-7nyy: Cryo-EM structure of the MukBEF monomer 手法: EM (単粒子) / 解像度: 6.8 Å 12659 7nyz EMDB-12659, PDB-7nyz: Cryo-EM structure of the MukBEF-MatP-DNA monomer (partially open conformation) 手法: EM (単粒子) / 解像度: 6.5 Å 12660 7nz0 EMDB-12660, PDB-7nz0: Cryo-EM structure of the MukBEF-MatP-DNA monomer (open conformation) 手法: EM (単粒子) / 解像度: 6.3 Å 12662 7nz2 EMDB-12662, PDB-7nz2: Cryo-EM structure of the MukBEF-MatP-DNA tetrad 手法: EM (単粒子) / 解像度: 11.0 Å 12663 7nz3 EMDB-12663, PDB-7nz3: Cryo-EM structure of apposed MukBEF-MatP monomers on DNA 手法: EM (単粒子) / 解像度: 11.0 Å 12664 7nz4 EMDB-12664, PDB-7nz4: Cryo-EM structure of the MukBEF dimer 手法: EM (単粒子) / 解像度: 13.0 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-PNS*: 4'-PHOSPHOPANTETHEINE / パンテテイン4′-りん酸 / Phosphopantetheine
由来	photorhabdus thracensis (バクテリア) escherichia coli bl21(de3) (大腸菌) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN (DNA結合タンパク質) / SMC-kleisin complex / ATPase (ATPアーゼ)