EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor.
ジャーナル・号・ページ	Cell Rep, Vol. 27, Issue 12, Page 3433-33446.e4, Year 2019
掲載日	2019年6月18日
著者	Célia Deville / Kamila Franke / Axel Mogk / Bernd Bukau / Helen R Saibil /
PubMed 要旨	AAA+ proteins form asymmetric hexameric rings that hydrolyze ATP and thread substrate proteins through a central channel via mobile substrate-binding pore loops. Understanding how ATPase and ...AAA+ proteins form asymmetric hexameric rings that hydrolyze ATP and thread substrate proteins through a central channel via mobile substrate-binding pore loops. Understanding how ATPase and threading activities are regulated and intertwined is key to understanding the AAA+ protein mechanism. We studied the disaggregase ClpB, which contains tandem ATPase domains (AAA1, AAA2) and shifts between low and high ATPase and threading activities. Coiled-coil M-domains repress ClpB activity by encircling the AAA1 ring. Here, we determine the mechanism of ClpB activation by comparing ATPase mechanisms and cryo-EM structures of ClpB wild-type and a constitutively active ClpB M-domain mutant. We show that ClpB activation reduces ATPase cooperativity and induces a sequential mode of ATP hydrolysis in the AAA2 ring, the main ATPase motor. AAA1 and AAA2 rings do not work synchronously but in alternating cycles. This ensures high grip, enabling substrate threading via a processive, rope-climbing mechanism.
リンク	Cell Rep / PubMed:31216466 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 6.2 Å
構造データ	4621 6qs4 EMDB-4621, PDB-6qs4: Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor. 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-4622: E. coli ClpB (DWB and K476C mutant) bound to casein - middle domain conformation 1 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-4623: E. coli ClpB (DWB and K476C mutant) bound to casein - middle domain conformation 2 手法: EM (単粒子) / 解像度: 3.8 Å 4624 6qs6 EMDB-4624, PDB-6qs6: ClpB (DWB and K476C mutant) bound to casein in presence of ATPgammaS - state KC-1 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-4625: E. coli ClpB (DWB and K476C mutant) bound to casein - state KC-2 手法: EM (単粒子) / 解像度: 3.4 Å 4626 6qs7 EMDB-4626, PDB-6qs7: ClpB (DWB and K476C mutant) bound to casein in presence of ATPgammaS - state KC-2A 手法: EM (単粒子) / 解像度: 3.8 Å 4627 6qs8 EMDB-4627, PDB-6qs8: ClpB (DWB and K476C mutant) bound to casein in presence of ATPgammaS - state KC-2B 手法: EM (単粒子) / 解像度: 3.9 Å 4940 6rn2 EMDB-4940, PDB-6rn2: ClpB (DWB mutant) bound to casein in presence of ATPgammaS - state WT-1 手法: EM (単粒子) / 解像度: 6.2 Å 4941 6rn3 EMDB-4941, PDB-6rn3: ClpB (DWB mutant) bound to casein in presence of ATPgammaS - state WT-2A 手法: EM (単粒子) / 解像度: 4.0 Å 4942 6rn4 EMDB-4942, PDB-6rn4: ClpB (DWB mutant) bound to casein in presence of ATPgammaS - state WT-2B 手法: EM (単粒子) / 解像度: 4.2 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	escherichia coli (大腸菌) escherichia coli (strain atcc 9637 / ccm 2024 / dsm 1116 / ncimb 8666 / nrrl b-766 / w) (大腸菌) bos taurus (ウシ) escherichia coli hvh 50 (4-2593475) (大腸菌)
キーワード	CHAPERONE (シャペロン) / disaggregase / proteostasis / AAA