EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Implications of a multiscale structure of the yeast nuclear pore complex.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 18, Page 3283-33302.e5, Year 2023
掲載日	2023年9月21日
著者	Christopher W Akey / Ignacia Echeverria / Christna Ouch / Ilona Nudelman / Yi Shi / Junjie Wang / Brian T Chait / Andrej Sali / Javier Fernandez-Martinez / Michael P Rout /
PubMed 要旨	Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from ...Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules. Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D density maps from cryogenic electron microscopy and AlphaFold2 models. Key features of the inner and outer rings were integrated into a comprehensive model. We resolved flexible connectors that tie together the core scaffold, along with equatorial transmembrane complexes and a lumenal ring that anchor this channel within the pore membrane. The organization of the nuclear double outer ring reveals an architecture that may be shared with ancestral NPCs. Additional connections between the core scaffold and the central transporter suggest that under certain conditions, a degree of local organization is present at the periphery of the transport machinery. These connectors may couple conformational changes in the scaffold to the central transporter to modulate transport. Collectively, this analysis provides insights into assembly, transport, and NPC evolution.
リンク	Mol Cell / PubMed:37738963 / PubMed Central
手法	EM (単粒子)
解像度	6.6 - 31.7 Å
構造データ	EMDB-41114: Nuclear double outer ring of the yeast NPC 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-41116: Lumenal ring of the isolated yeast NPC 手法: EM (単粒子) / 解像度: 14.7 Å 41117 8t9l EMDB-41117, PDB-8t9l: Pom34-Pom152 membrane attachment site yeast NPC 手法: EM (単粒子) / 解像度: 7.0 Å EMDB-41119: Cytoplasmic outer ring of the yeast NPC 手法: EM (単粒子) / 解像度: 22.0 Å EMDB-41120: Central transporter/plug yeast NPC 手法: EM (単粒子) / 解像度: 28.0 Å EMDB-41121: Double outer ring connectors of the yeast NPC 手法: EM (単粒子) / 解像度: 29.5 Å EMDB-41122: Connectors in cytoplasmic outer ring of yeast NPC 手法: EM (単粒子) / 解像度: 31.7 Å EMDB-41123: Composite multiscale structure of the yeast NPC 手法: EM (単粒子) / 解像度: 28.0 Å 41285 8tie EMDB-41285, PDB-8tie: Double nuclear outer ring of Nup84-complexes from the yeast NPC 手法: EM (単粒子) / 解像度: 8.1 Å 41300 8tj5 EMDB-41300, PDB-8tj5: Inner spoke ring of the yeast NPC 手法: EM (単粒子) / 解像度: 6.6 Å
由来	saccharomyces cerevisiae (パン酵母)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / nuclear pore complex (核膜孔) / nucleocytoplasmic transport / nucleoporin (ヌクレオポリン) / membrane protein (膜タンパク質) / translocase (輸送酵素)