EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of human γδ T cell receptor-CD3 complex.
ジャーナル・号・ページ	Nature, Year 2024
掲載日	2024年4月24日
著者	Weizhi Xin / Bangdong Huang / Ximin Chi / Yuehua Liu / Mengjiao Xu / Yuanyuan Zhang / Xu Li / Qiang Su / Qiang Zhou /
PubMed 要旨	Gamma delta (γδ) T cells, a unique T cell subgroup, are crucial in various immune responses and immunopathology. The γδ T cell receptor (TCR), which is generated by γδ T cells, recognizes a ...Gamma delta (γδ) T cells, a unique T cell subgroup, are crucial in various immune responses and immunopathology. The γδ T cell receptor (TCR), which is generated by γδ T cells, recognizes a diverse range of antigens independently of the major histocompatibility complex. The γδ TCR associates with CD3 subunits, initiating T cell activation and holding great potential in immunotherapy. Here we report the structures of two prototypical human Vγ9Vδ2 and Vγ5Vδ1 TCR-CD3 complexes, revealing two distinct assembly mechanisms that depend on Vγ usage. The Vγ9Vδ2 TCR-CD3 complex is monomeric, with considerable conformational flexibility in the TCRγ-TCRδ extracellular domain and connecting peptides. The length of the connecting peptides regulates the ligand association and T cell activation. A cholesterol-like molecule wedges into the transmembrane region, exerting an inhibitory role in TCR signalling. The Vγ5Vδ1 TCR-CD3 complex displays a dimeric architecture, whereby two protomers nestle back to back through the Vγ5 domains of the TCR extracellular domains. Our biochemical and biophysical assays further corroborate the dimeric structure. Importantly, the dimeric form of the Vγ5Vδ1 TCR is essential for T cell activation. These findings reveal organizing principles of the γδ TCR-CD3 complex, providing insights into the unique properties of γδ TCR and facilitating immunotherapeutic interventions.
リンク	Nature / PubMed:38657677
手法	EM (単粒子)
解像度	3.0 - 9.5 Å
構造データ	36147 8jbv EMDB-36147, PDB-8jbv: Extracellular domain of gamma delta TCR 手法: EM (単粒子) / 解像度: 3.02 Å 36149 8jc0 EMDB-36149, PDB-8jc0: V gamma9 V delta2 TCR and CD3 complex in LMNG 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-36152: Vgamma5 Vdelta1 TCR complex (MPDI/TMDI) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-36153: Vgamma5 Vdelta1 TCR complex (MPDII/TMDII) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-36155: Vgamma5 Vdelta1 TCR complex (MPD/TMD) 手法: EM (単粒子) / 解像度: 5.7 Å 36156 8jcb EMDB-36156, PDB-8jcb: Vgamma5 Vdelta1 T cell receptor complex 手法: EM (単粒子) / 解像度: 9.5 Å 37904 8wxe EMDB-37904, PDB-8wxe: Vgamma5Vdelta1 EH TCR-CD3 complex 手法: EM (単粒子) / 解像度: 4.0 Å 37914 8wy0 EMDB-37914, PDB-8wy0: T cell receptor delta 2 gamma 9 with F283A, F290A, and F291A 手法: EM (単粒子) / 解像度: 3.8 Å 37929 8wyi EMDB-37929, PDB-8wyi: T cell receptor delta 2 gamma 9 with TCRD TM domain chimera of TRAC 手法: EM (単粒子) / 解像度: 3.9 Å 39128 8yc0 EMDB-39128, PDB-8yc0: T cell receptor V delta2 V gamma9 in GDN 手法: EM (単粒子) / 解像度: 4.12 Å EMDB-39359: T cell receptor V delta2 V gamma9 in GDN (DeepEMhancer) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-39361: Vgamma5 Vdelta1 TCR complex (MPDI/TMDI, DeepEMhancer) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-39362: Vgamma5 Vdelta1 TCR complex (MPDII/TMDII, DeepEMhancer) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-39363: V gamma9 V delta2 TCR and CD3 complex in LMNG (DeepEMhancer) 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-39367: Vgamma5 Vdelta1 TCR-CD3 complex (EH mutant, DeepEMhancer) 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-39368: Extracellular domain of Vgamma5 Vdelta1 TCR (DeepEMhancer) 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-CLR: CHOLESTEROL / コレステロ-ル / コレステロール
由来	homo sapiens (ヒト)
キーワード	IMMUNE SYSTEM (免疫系) / Receptor / immunity / immune