EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Euglena's atypical respiratory chain adapts to the discoidal cristae and flexible metabolism.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 1628, Year 2024
掲載日	2024年2月22日
著者	Zhaoxiang He / Mengchen Wu / Hongtao Tian / Liangdong Wang / Yiqi Hu / Fangzhu Han / Jiancang Zhou / Yong Wang / Long Zhou /
PubMed 要旨	Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron ...Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron transport chain. While structures of the electron transport chain complexes and supercomplexes of most other eukaryotic clades have been reported, no similar structure is currently available for Discoba, limiting the understandings of its core metabolism and leaving a gap in the evolutionary tree of eukaryotic bioenergetics. Here, we report high-resolution cryo-EM structures of Euglena's respirasome I + III + IV and supercomplex III + IV. A previously unreported fatty acid synthesis domain locates on the tip of complex I's peripheral arm, providing a clear picture of its atypical subunit composition identified previously. Individual complexes are re-arranged in the respirasome to adapt to the non-uniform membrane curvature of the discoidal cristae. Furthermore, Euglena's conformationally rigid complex I is deactivated by restricting ubiquinone's access to its substrate tunnel. Our findings provide structural insights for therapeutic developments against euglenozoan parasite infections.
リンク	Nat Commun / PubMed:38388527 / PubMed Central
手法	EM (単粒子)
解像度	2.69 - 3.14 Å
構造データ	EMDB-35662: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex I peripheral arm focused 手法: EM (単粒子) / 解像度: 2.73 Å EMDB-35663: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex I proximal membrane arm focused 手法: EM (単粒子) / 解像度: 2.72 Å EMDB-35664: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex I distal membrane arm focused 手法: EM (単粒子) / 解像度: 2.69 Å EMDB-35665: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex III2 focused 手法: EM (単粒子) / 解像度: 2.77 Å EMDB-35666: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex IV focused 手法: EM (単粒子) / 解像度: 2.76 Å EMDB-35667: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, complex III2 focused 手法: EM (単粒子) / 解像度: 2.85 Å EMDB-35668: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, complex IV focused 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-35669: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, complex IV focused 手法: EM (単粒子) / 解像度: 3.14 Å 35720 8iuf EMDB-35720, PDB-8iuf: Cryo-EM structure of Euglena gracilis super-complex I+III2+IV, composite 手法: EM (単粒子) / 解像度: 2.81 Å 35723 8iuj EMDB-35723: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, composite PDB-8iuj: Cryo-EM structure of Euglena gracilis super-complex III2+IV2, composite 手法: EM (単粒子) / 解像度: 3.06 Å EMDB-35819: Cryo-EM map of Euglena gracilis respirasome I+III2+IV 手法: EM (単粒子) / 解像度: 2.81 Å EMDB-35820: Cryo-EM map of Euglena gracilis supercomplex III2+IV2 手法: EM (単粒子) / 解像度: 3.06 Å EMDB-36094: Cryo-EM map of Euglena gracilis respiratory complex I in the deactive state, peripheral arm focused 手法: EM (単粒子) / 解像度: 3.07 Å EMDB-36096: Cryo-EM map of Euglena gracilis respiratory complex I in the deactive state, proximal membrane arm focused 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-36097: Cryo-EM map of Euglena gracilis respiratory complex I in the deactive state, distal membrane arm focused 手法: EM (単粒子) / 解像度: 3.03 Å EMDB-36098: Cryo-EM consensus map of Euglena gracilis respiratory complex I, deactive state 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-36099: Cryo-EM map of Euglena gracilis respiratory complex I in the turnover state, peripheral arm focused 手法: EM (単粒子) / 解像度: 2.82 Å EMDB-36100: Cryo-EM map of Euglena gracilis respiratory complex I in the turnover state, proximal membrane arm focused 手法: EM (単粒子) / 解像度: 2.87 Å EMDB-36101: Cryo-EM map of Euglena gracilis respiratory complex I in the turnover state, distal membrane arm focused 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-36102: Cryo-EM consensus map of Euglena gracilis respiratory complex I, turnover state 手法: EM (単粒子) / 解像度: 2.87 Å EMDB-36103: Cryo-EM map of Euglena gracilis respiratory complex I in the NADH state, peripheral arm focused 手法: EM (単粒子) / 解像度: 2.97 Å EMDB-36104: Cryo-EM map of Euglena gracilis respiratory complex I in the NADH state, proximal membrane arm focused 手法: EM (単粒子) / 解像度: 2.93 Å EMDB-36105: Cryo-EM map of Euglena gracilis respiratory complex I in the NADH state, distal membrane focused 手法: EM (単粒子) / 解像度: 2.94 Å EMDB-36106: Cryo-EM consensus map of Euglena gracilis respiratory complex I, NADH state 手法: EM (単粒子) / 解像度: 3.03 Å 36107 8j9h EMDB-36107: Cryo-EM composite map of Euglena gracilis respiratory complex I, deactive state PDB-8j9h: Cryo-EM structure of Euglena gracilis respiratory complex I, deactive state 手法: EM (単粒子) / 解像度: 3.11 Å 36108 8j9i EMDB-36108: Cryo-EM composite map of Euglena gracilis complex I, turnover state PDB-8j9i: Cryo-EM structure of Euglena gracilis complex I, turnover state 手法: EM (単粒子) / 解像度: 2.87 Å 36109 8j9j EMDB-36109: Cryo-EM composite map of Euglena gracilis complex I, NADH state PDB-8j9j: Cryo-EM structure of Euglena gracilis complex I, NADH state 手法: EM (単粒子) / 解像度: 3.03 Å
化合物	ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / 鉄・硫黄クラスター ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-K: Unknown entry / カリウムカチオン ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-S12: O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-ZN: Unknown entry ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH / ニコチンアミドアデニンジヌクレオチドリン酸 ChemComp-ZMP: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate ChemComp-HEA: HEME-A / Heme A ChemComp-CU: COPPER (II) ION / 銅 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PX2: 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE ChemComp-U10: UBIQUINONE-10 / 2-(3,7,11,15,19,23,27,31,35,39-デカメチルテトラコンタ-2,6,10,14,18,22,(以下略) / ユビキノン ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE / Heme B ChemComp-HEC: HEME C / Heme C ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN / フラビンモノヌクレオチド ChemComp-NAI*: 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE / NADH / ニコチンアミドアデニンジヌクレオチド
由来	euglena gracilis (ミドリムシ)
キーワード	ELECTRON TRANSPORT (電子伝達系) / Electron transport chain (電子伝達系) / supercomplex / membrane protein (膜タンパク質) / Euglena gracilis / complex / respiratory complex (呼吸器)