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-Structure paper
タイトル | Structural basis of membrane skeleton organization in red blood cells. |
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ジャーナル・号・ページ | Cell, Vol. 186, Issue 9, Page 1912-1929.e18, Year 2023 |
掲載日 | 2023年4月27日 |
著者 | Ningning Li / Siyi Chen / Kui Xu / Meng-Ting He / Meng-Qiu Dong / Qiangfeng Cliff Zhang / Ning Gao / |
PubMed 要旨 | The spectrin-based membrane skeleton is a ubiquitous membrane-associated two-dimensional cytoskeleton underneath the lipid membrane of metazoan cells. Mutations of skeleton proteins impair the ...The spectrin-based membrane skeleton is a ubiquitous membrane-associated two-dimensional cytoskeleton underneath the lipid membrane of metazoan cells. Mutations of skeleton proteins impair the mechanical strength and functions of the membrane, leading to several different types of human diseases. Here, we report the cryo-EM structures of the native spectrin-actin junctional complex (from porcine erythrocytes), which is a specialized short F-actin acting as the central organizational unit of the membrane skeleton. While an α-/β-adducin hetero-tetramer binds to the barbed end of F-actin as a flexible cap, tropomodulin and SH3BGRL2 together create an absolute cap at the pointed end. The junctional complex is strengthened by ring-like structures of dematin in the middle actin layers and by patterned periodic interactions with tropomyosin over its entire length. This work serves as a structural framework for understanding the assembly and dynamics of membrane skeleton and offers insights into mechanisms of various ubiquitous F-actin-binding factors in other F-actin systems. |
リンク | Cell / PubMed:37044097 |
手法 | EM (単粒子) |
解像度 | 2.96 - 9.5 Å |
構造データ | EMDB-35301, PDB-8iah: EMDB-35302, PDB-8iai: EMDB-35303: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment EMDB-35305: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Central segment EMDB-35307: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment, TMCC1 optimized EMDB-35308: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Pointed-end segment, TMCC2 optimized EMDB-35311: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Central segment, TMCC1 optimized EMDB-35312: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, Central segment, TMCC2 optimized EMDB-35317: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment EMDB-35318: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, adducin optimized EMDB-35319: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, TMCC1 optimized EMDB-35320: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, TMCC2 optimized EMDB-35321: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, the second spectrin repeat dimer optimized EMDB-35322: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, the first two spectrin repeat dimers optimized EMDB-35324: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Barbed-end segment, the third to fifth spectrin repeat dimers optimized EMDB-35325: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Barbed-end segment EMDB-35327: Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State II, Barbed-end segment, adducin/TMCC1 optimized EMDB-35329, PDB-8ib2: |
化合物 | ChemComp-ADP: |
由来 |
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キーワード | MEMBRANE PROTEIN / Macrocomplex / membrane skeleton / spectrin-actin junction |