EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The open gate of the AMPA receptor forms a Ca binding site critical in regulating ion transport.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 4, Page 688-700, Year 2024
掲載日	2024年2月26日
著者	Terunaga Nakagawa / Xin-Tong Wang / Federico J Miguez-Cabello / Derek Bowie /
PubMed 要旨	Alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionic acid receptors (AMPARs) are cation-selective ion channels that mediate most fast excitatory neurotransmission in the brain. Although their gating ...Alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionic acid receptors (AMPARs) are cation-selective ion channels that mediate most fast excitatory neurotransmission in the brain. Although their gating mechanism has been studied extensively, understanding how cations traverse the pore has remained elusive. Here we investigated putative ion and water densities in the open pore of Ca-permeable AMPARs (rat GRIA2 flip-Q isoform) at 2.3-2.6 Å resolution. We show that the ion permeation pathway attains an extracellular Ca binding site (site-G) when the channel gate moves into the open configuration. Site-G is highly selective for Ca over Na, favoring the movement of Ca into the selectivity filter of the pore. Seizure-related N619K mutation, adjacent to site-G, promotes channel opening but attenuates Ca binding and thus diminishes Ca permeability. Our work identifies the importance of site-G, which coordinates with the Q/R site of the selectivity filter to ensure the preferential transport of Ca through the channel pore.
リンク	Nat Struct Mol Biol / PubMed:38409505
手法	EM (単粒子)
解像度	2.29 - 5.59 Å
構造データ	29359 8fp4 EMDB-29359, PDB-8fp4: GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 500mM NaCl, 330uM CTZ, and 100mM glutamate (Open-Na610) 手法: EM (単粒子) / 解像度: 2.4 Å 29360 8fp9 EMDB-29360, PDB-8fp9: GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100mM glutamate (Open-CaNaMg) 手法: EM (単粒子) / 解像度: 2.44 Å 29361 8fpc EMDB-29361, PDB-8fpc: Conformation 1 of the ligand binding domain (LBDconf1) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100mM glutamate (Open-CaNaMg) 手法: EM (単粒子) / 解像度: 2.78 Å 29363 8fpg EMDB-29363, PDB-8fpg: GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100uM CNQX (Closed-CaNaMg) 手法: EM (単粒子) / 解像度: 2.32 Å 29364 8fph EMDB-29364, PDB-8fph: Conformation 2 of the ligand binding domain (LBDconf2) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100mM glutamate (Open-CaNaMg) 手法: EM (単粒子) / 解像度: 3.14 Å 29367 8fpk EMDB-29367, PDB-8fpk: LBD conformation 1 of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100uM CNQX (Closed-CaNaMg) 手法: EM (単粒子) / 解像度: 2.76 Å 29368 8fpl EMDB-29368, PDB-8fpl: LBD conformation 2 (LBDconf2) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100uM CNQX (Closed-CaNaMg) 手法: EM (単粒子) / 解像度: 2.81 Å 29369 8fps EMDB-29369, PDB-8fps: GluA2 flip Q isoform N619K mutant of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100mM glutamate (Open-CaNaMg/N619K) 手法: EM (単粒子) / 解像度: 2.38 Å 29370 8fpv EMDB-29370, PDB-8fpv: LBD conformation 1 (LBDconf1) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 500mM NaCl, 330uM CTZ, and 100mM glutamate (Open-Na610) 手法: EM (単粒子) / 解像度: 3.08 Å 29371 8fpy EMDB-29371, PDB-8fpy: LBD conformation 2 (LBDconf2) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 500mM NaCl, 330uM CTZ, and 100mM glutamate (Open-Na610) 手法: EM (単粒子) / 解像度: 2.98 Å 29372 8fpz EMDB-29372, PDB-8fpz: LBD conformation 3 (LBDconf3) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 500mM NaCl, 330uM CTZ, and 100mM glutamate (Open-Na610) 手法: EM (単粒子) / 解像度: 3.01 Å 29373 8fq1 EMDB-29373, PDB-8fq1: GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 150mM CaCl2, 330uM CTZ, and 100mM L-glutamate (Open-Ca150) 手法: EM (単粒子) / 解像度: 5.59 Å 29375 8fq2 EMDB-29375, PDB-8fq2: LBD conformation 1 (LBDconf1) of GluA2 flip Q isoform N619K mutant of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100mM glutamate (Open-CaNaMg/N619K) 手法: EM (単粒子) / 解像度: 3.14 Å 29376 8fq3 EMDB-29376, PDB-8fq3: LBD conformation 2 (LBDconf2) of GluA2 flip Q isoform N619K mutant of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100mM glutamate (Open-CaNaMg/N619K) 手法: EM (単粒子) / 解像度: 3.17 Å 29378 8fq5 EMDB-29378, PDB-8fq5: GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 140mM NMDG, 330uM CTZ, and 100mM L-glutamate (Open-Na110) 手法: EM (単粒子) / 解像度: 2.34 Å 29379 8fq6 EMDB-29379, PDB-8fq6: LBD of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 150mM CaCl2, 330uM CTZ, and 100mM L-glutamate (Open-Ca150) 手法: EM (単粒子) / 解像度: 3.14 Å 29380 8fq8 EMDB-29380, PDB-8fq8: LBD conformation 1 (LBDconf1) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 140mM NMDG, 330uM CTZ, and 100mM L-glutamate (Open-Na110) 手法: EM (単粒子) / 解像度: 3.11 Å 29381 8fqa EMDB-29381, PDB-8fqa: LBD conformation 2 (LBDconf2) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 140mM NMDG, 330uM CTZ, and 100mM L-glutamate (Open-Na110) 手法: EM (単粒子) / 解像度: 3.35 Å 29382 8fqb EMDB-29382, PDB-8fqb: GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 10mM CaCl2, 140mM NMDG, 330uM CTZ, and 100mM L-glutamate (Open-Ca10) 手法: EM (単粒子) / 解像度: 2.36 Å 29384 8fqd EMDB-29384, PDB-8fqd: LBD conformation 1 (LBDconf1) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 10mM CaCl2, 140mM NMDG, 330uM CTZ, and 100mM L-glutamate (Open-Ca10) 手法: EM (単粒子) / 解像度: 3.01 Å 29385 8fqe EMDB-29385, PDB-8fqe: LBD conformation 2 (LBDconf2) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 10mM CaCl2, 140mM NMDG, 330uM CTZ, and 100mM L-glutamate (Open-Ca10) 手法: EM (単粒子) / 解像度: 3.14 Å 29386 8fqf EMDB-29386, PDB-8fqf: GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 150mM NaCl, 330uM CTZ, and 100mM glutamate (Open-Na260) 手法: EM (単粒子) / 解像度: 2.29 Å 29387 8fqg EMDB-29387, PDB-8fqg: LBD conformation 1 (LBDconf1) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 150mM NaCl, 330uM CTZ, and 100mM glutamate (Open-Na260) 手法: EM (単粒子) / 解像度: 3.01 Å 29388 8fqh EMDB-29388, PDB-8fqh: LBD conformation 2 (LBDconf2) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 150mM NaCl, 330uM CTZ, and 100mM glutamate (Open-Na260) 手法: EM (単粒子) / 解像度: 3.43 Å 29394 8fr0 EMDB-29394, PDB-8fr0: LBD conformation 3 (LBDconf3) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 150mM NaCl, 330uM CTZ, and 100mM glutamate (Open-Na260) 手法: EM (単粒子) / 解像度: 3.04 Å
化合物	ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-HOH: WATER / 水 ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-GLU: GLUTAMIC ACID / グルタミン酸 / グルタミン酸 ChemComp-CYZ: CYCLOTHIAZIDE / (3S)-6-クロロ-3,4-ジヒドロ-3-[(1S,4S,5S)-ビシクロ[2.2.1]ヘプタ-2-エン-5-イル](以下略) / Cyclothiazide 化合物画像なし ChemComp-Y4Y: Unknown entry
由来	Rattus (クマネズミ属) rattus norvegicus (ドブネズミ) mus musculus (ハツカネズミ)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / ionotropic glutamate receptor / ligand gated ion channel (リガンド依存性イオンチャネル) / AMPA receptor (AMPA型グルタミン酸受容体) / TARP gamma-2 / ionotropic glutamate receptors / AMPA receptors / ion channel (イオンチャネル) / ligand-gated ion channel (リガンド依存性イオンチャネル) / auxiliary subunit / stargazing / TARP gamma2 / Inotropic glutamate receptors / TARP / stargazin / glutamate (グルタミン酸) / calcium (カルシウム) / neurotransmitter receptor (神経伝達物質受容体) / synaptic transmission