EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 3793, Year 2022
掲載日	2022年7月1日
著者	Brady A Travis / Jared V Peck / Raul Salinas / Brandon Dopkins / Nicholas Lent / Viet D Nguyen / Mario J Borgnia / Richard G Brennan / Maria A Schumacher /
PubMed 要旨	How bacteria sense and respond to nitrogen levels are central questions in microbial physiology. In Gram-positive bacteria, nitrogen homeostasis is controlled by an operon encoding glutamine ...How bacteria sense and respond to nitrogen levels are central questions in microbial physiology. In Gram-positive bacteria, nitrogen homeostasis is controlled by an operon encoding glutamine synthetase (GS), a dodecameric machine that assimilates ammonium into glutamine, and the GlnR repressor. GlnR detects nitrogen excess indirectly by binding glutamine-feedback-inhibited-GS (FBI-GS), which activates its transcription-repression function. The molecular mechanisms behind this regulatory circuitry, however, are unknown. Here we describe biochemical and structural analyses of GS and FBI-GS-GlnR complexes from pathogenic and non-pathogenic Gram-positive bacteria. The structures show FBI-GS binds the GlnR C-terminal domain within its active-site cavity, juxtaposing two GlnR monomers to form a DNA-binding-competent GlnR dimer. The FBI-GS-GlnR interaction stabilizes the inactive GS conformation. Strikingly, this interaction also favors a remarkable dodecamer to tetradecamer transition in some GS, breaking the paradigm that all bacterial GS are dodecamers. These data thus unveil unique structural mechanisms of transcription and enzymatic regulation.
リンク	Nat Commun / PubMed:35778410 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.96 - 3.5 Å
構造データ	25863 7tf6 EMDB-25863, PDB-7tf6: S. aureus GS(12)-Q-GlnR peptide 手法: EM (単粒子) / 解像度: 2.15 Å 25864 7tf7 EMDB-25864, PDB-7tf7: S. aureus GS(12) - apo 手法: EM (単粒子) / 解像度: 2.13 Å 25866 7tf9 EMDB-25866, PDB-7tf9: L. monocytogenes GS(14)-Q-GlnR peptide 手法: EM (単粒子) / 解像度: 2.61 Å 25867 7tfa EMDB-25867, PDB-7tfa: P. polymyxa GS(12)-Q-GlnR peptide 手法: EM (単粒子) / 解像度: 2.07 Å 25868 7tfb EMDB-25868, PDB-7tfb: P. polymyxa GS(14)-Q-GlnR peptide 手法: EM (単粒子) / 解像度: 2.28 Å 25869 7tfc EMDB-25869, PDB-7tfc: B. subtilis GS(14)-Q-GlnR peptide 手法: EM (単粒子) / 解像度: 1.96 Å 25870 7tfd EMDB-25870, PDB-7tfd: P. polymyxa GS(12) - apo 手法: EM (単粒子) / 解像度: 3.16 Å 25871 7tfe EMDB-25871, PDB-7tfe: L. monocytogenes GS(12) - apo 手法: EM (単粒子) / 解像度: 2.85 Å PDB-7tdp: Structure of Paenibacillus polymyxa GS bound to Met-Sox-P-ADP (Transition state complex) to 1.98 Angstom 手法: X-RAY DIFFRACTION / 解像度: 1.98 Å PDB-7tdv: Crystal structure of S. aureus glutamine synthetase in Met-Sox-P/ADP transition state complex 手法: X-RAY DIFFRACTION / 解像度: 2.92 Å PDB-7tea: Crystal structure of S. aureus GlnR-DNA complex 手法: X-RAY DIFFRACTION / 解像度: 2.35 Å PDB-7tec: Structure of the Listeria monocytogenes GlnR-DNA complex to 3.45 Angstrom 手法: X-RAY DIFFRACTION / 解像度: 3.45 Å PDB-7ten: Crystal structure of the Listeria monocytogenes GS-Met-Sox-P- ADP complex to 3.5 Angstrom 手法: X-RAY DIFFRACTION / 解像度: 3.5 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-P3S: L-METHIONINE-S-SULFOXIMINE PHOSPHATE ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER / 水 ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-GLN*: GLUTAMINE / グルタミン / グルタミン
由来	staphylococcus aureus (黄色ブドウ球菌) listeria monocytogenes (リステリア・モノサイトゲネス) paenibacillus polymyxa (パエニバシラス・ポリミキサ) bacillus subtilis (枯草菌) synthetic construct (人工物)
キーワード	LIGASE (リガーゼ) / glutamate-ammonium ligase / GlnR / GS / feedback inhibition (酵素阻害剤) / transcription coregulator / glnRA / Glutamine synthetase (グルタミンシンテターゼ) / S. aureus / femC / DNA BINDING PROTEIN/DNA / winged-HTH / transcription repressor / DNA BINDING PROTEIN (DNA結合タンパク質) / DNA BINDING PROTEIN-DNA complex / listeria (リステリア) / repressor (リプレッサー) / transcription (転写 (生物学)) / LIGASE/INHIBITOR / glutamate ammonium ligase / Met-Sox / transition state (遷移状態) / LIGASE-INHIBITOR complex / BIOSYNTHETIC PROTEIN (生合成) / glutamine synthetase repressor dodecamer / glutamine synthetase dodecamer / glutamine synthetase repressor tetradecamer