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TitleDistinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausing.
Journal, issue, pagesMol Cell, Vol. 84, Issue 7, Page 1243-11256.e5, Year 2024
Publish dateApr 4, 2024
AuthorsBonnie G Su / Seychelle M Vos /
PubMed AbstractMetazoan gene expression regulation involves pausing of RNA polymerase (Pol II) in the promoter-proximal region of genes and is stabilized by DSIF and NELF. Upon depletion of elongation factors, NELF ...Metazoan gene expression regulation involves pausing of RNA polymerase (Pol II) in the promoter-proximal region of genes and is stabilized by DSIF and NELF. Upon depletion of elongation factors, NELF appears to accompany elongating Pol II past pause sites; however, prior work indicates that NELF prevents Pol II elongation. Here, we report cryoelectron microscopy structures of Pol II-DSIF-NELF complexes with NELF in two distinct conformations corresponding to paused and poised states. The paused NELF state supports Pol II stalling, whereas the poised NELF state enables transcription elongation as it does not support a tilted RNA-DNA hybrid. Further, the poised NELF state can accommodate TFIIS binding to Pol II, allowing for Pol II reactivation at paused or backtracking sites. Finally, we observe that the NELF-A tentacle interacts with the RPB2 protrusion and is necessary for pausing. Our results define how NELF can support pausing, reactivation, and elongation by Pol II.
External linksMol Cell / PubMed:38401543 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 3.5 Å
Structure data

42267

8uha

EMDB-42267, PDB-8uha:
Structure of paused transcription complex Pol II-DSIF-NELF - tilted
Method: EM (single particle) / Resolution: 3.5 Å

42270

8uhd

EMDB-42270, PDB-8uhd:
Structure of paused transcription complex Pol II-DSIF-NELF - post-translocated
Method: EM (single particle) / Resolution: 2.8 Å

42280

8uhg

EMDB-42280, PDB-8uhg:
Structure of paused transcription complex Pol II-DSIF-NELF - poised post-translocated
Method: EM (single particle) / Resolution: 2.7 Å

42285

8ui0

EMDB-42285, PDB-8ui0:
Structure of poised transcription complex Pol II-DSIF-NELF - pre-translocated
Method: EM (single particle) / Resolution: 2.7 Å

42303

8uis

EMDB-42303, PDB-8uis:
Structure of transcription complex Pol II-DSIF-NELF-TFIIS
Method: EM (single particle) / Resolution: 3.23 Å

EMDB-42304: Structure of Pol II-DSIF-NELF - post-translocated
Method: EM (single particle) / Resolution: 2.8 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
  • sus scrofa (pig)
  • synthetic construct (others)
KeywordsTRANSCRIPTION/DNA/RNA / Nucleic acids / transcription / RNA polymerase II / NELF / DSIF / pausing / TRANSCRIPTION-DNA-RNA complex / TRANSCRIPTION/RNA/DNA / TRANSCRIPTION-RNA-DNA complex

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