Title A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers. Journal, issue, pages Nat Commun , Vol. 14, Page 7956-7956, Year 2023Publish date Feb 16, 2023 (structure data deposition date) AuthorsWranik, M. / Kepa, M.W. / Beale, E.V. / James, D. / Bertrand, Q. / Weinert, T. / Furrer, A. / Glover, H. / Gashi, D. / Carrillo, M. ...Wranik, M. / Kepa, M.W. / Beale, E.V. / James, D. / Bertrand, Q. / Weinert, T. / Furrer, A. / Glover, H. / Gashi, D. / Carrillo, M. / Kondo, Y. / Stipp, R.T. / Khusainov, G. / Nass, K. / Ozerov, D. / Cirelli, C. / Johnson, P.J.M. / Dworkowski, F. / Beale, J.H. / Stubbs, S. / Zamofing, T. / Schneider, M. / Krauskopf, K. / Gao, L. / Thorn-Seshold, O. / Bostedt, C. / Bacellar, C. / Steinmetz, M.O. / Milne, C. / Standfuss, J. External links Nat Commun / PubMed:38042952Methods X-ray diffraction Resolution 1.31 - 3.2 Å Structure data PDB-8cl5 : Structure viewer Lysozyme in matrix of lipidic cubic phase of monoolein (LCP) Method : X-RAY DIFFRACTION / Resolution : 1.31 Å
PDB-8cl6 : Structure viewer Lysozyme in matrix of hydroxyethylcellulose (HEC) Method : X-RAY DIFFRACTION / Resolution : 1.32 Å
PDB-8cl7 : Structure viewer Krokinobacter eikastus rhodopsin 2 (KR2) in dark state Method : X-RAY DIFFRACTION / Resolution : 1.76 Å
PDB-8cl8 : Structure viewer Krokinobacter eikastus rhodopsin 2 (KR2) extrapolated map 1us after light activation Method : X-RAY DIFFRACTION / Resolution : 2.4 Å
PDB-8cl9 : Structure viewerTubulin-DARPin D1 complexMethod : X-RAY DIFFRACTION / Resolution : 2.5 Å
PDB-8clb : Structure viewerColchicine bound to tubulin (T2R-TTL) complexMethod : X-RAY DIFFRACTION / Resolution : 3.0 Å
PDB-8clc : Structure viewerTubulin (T2R-TTL) complexMethod : X-RAY DIFFRACTION / Resolution : 2.7 Å
PDB-8cld : Structure viewerAnsamitocin P3 bound to tubulin (T2R-TTL) complexMethod : X-RAY DIFFRACTION / Resolution : 3.2 Å
PDB-8cle : Structure viewerVinblastine bound to tubulin (T2R-TTL) complexMethod : X-RAY DIFFRACTION / Resolution : 3.2 Å
PDB-8clf : Structure viewerZ-SolQ2Br bound to tubulin (T2R-TTL) complexMethod : X-RAY DIFFRACTION / Resolution : 2.7 Å
PDB-8clg : Structure viewerEpothilone A and Colchicine bound to tubulin (T2R-TTL) complexMethod : X-RAY DIFFRACTION / Resolution : 2.8 Å
PDB-8clh : Structure viewerDrug cocktail (Colchicine, Epothilone A, Peloruside, Ansamitocin P3, Vinblastine) bound to tubulin (T2R-TTL) complexMethod : X-RAY DIFFRACTION / Resolution : 2.5 Å
Chemicals ChemComp-EDO : Structure viewer 1,2-ETHANEDIOL / Ethylene glycol
ChemComp-1PE : Structure viewer PENTAETHYLENE GLYCOL / precipitant*YM / Polyethylene glycol
ChemComp-GTP : Structure viewer GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate
ChemComp-GDP : Structure viewer GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate
ChemComp-LOC : Structure viewer N-[(7S)-1,2,3,10-tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[d]heptalen-7-yl]ethanamide / medication, antiinflammatory*YM / Colchicine
ChemComp-ACP : Structure viewer PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM
ChemComp-BKF : Structure viewer (1S,2S,3S,5S,6S,16Z,18Z,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl 2-methylpropanoate
ChemComp-VLB : Structure viewer (2ALPHA,2'BETA,3BETA,4ALPHA,5BETA)-VINCALEUKOBLASTINE / medication, chemotherapy*YM / Vinblastine
ChemComp-V1O : Structure viewer 5-[[4-(2-bromoethyl)-3,5-dimethoxy-phenyl]diazenyl]-2-methoxy-phenol
Source asplenium bulbiferum subsp. bulbiferum (hen and chickens fern)dokdonia eikasta (bacteria)synthetic construct (others) bos taurus (cattle)mus musculus (house mouse)pavo cristatus (Indian peafowl)rattus norvegicus (Norway rat)gallus gallus (chicken) Show more 4 items Show less Keywords HYDROLASE / lysozyme / lysozyme activity / catalytic activity / hydrolase activity / MEMBRANE PROTEIN / KR2 / Light driven sodium pump / Sodium Pump / Photoactivation / Photocycle / CELL CYCLE / Drug-tubulin-complex Cell cycle inhibition