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Structure paper

TitleStructure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions.
Journal, issue, pagesScience, Vol. 350, Issue 6260, Page aab4070, Year 2015
Publish dateOct 30, 2015
AuthorsJiansen Jiang / Henry Chan / Darian D Cash / Edward J Miracco / Rachel R Ogorzalek Loo / Heather E Upton / Duilio Cascio / Reid O'Brien Johnson / Kathleen Collins / Joseph A Loo / Z Hong Zhou / Juli Feigon /
PubMed AbstractTelomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-ends, using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). We report the ...Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-ends, using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). We report the cryo-electron microscopy structure of Tetrahymena telomerase at ~9 angstrom resolution. In addition to seven known holoenzyme proteins, we identify two additional proteins that form a complex (TEB) with single-stranded telomere DNA-binding protein Teb1, paralogous to heterotrimeric replication protein A (RPA). The p75-p45-p19 subcomplex is identified as another RPA-related complex, CST (CTC1-STN1-TEN1). This study reveals the paths of TER in the TERT-TER-p65 catalytic core and single-stranded DNA exit; extensive subunit interactions of the TERT essential N-terminal domain, p50, and TEB; and other subunit identities and structures, including p19 and p45C crystal structures. Our findings provide structural and mechanistic insights into telomerase holoenzyme function.
External linksScience / PubMed:26472759 / PubMed Central
MethodsEM (single particle) / X-ray diffraction / NMR (solution)
Resolution2.301 - 9.4 Å
Structure data

EMDB-6442:
CryoEM structure of endogenously assembled Tetrahymena telomerase holoenzyme at 9.4 Angstrom resolution
Method: EM (single particle) / Resolution: 9.4 Å

EMDB-6443:
CryoEM structure of endogenously assembled Tetrahymena telomerase holoenzyme at 8.9 Angstrom resolution
Method: EM (single particle) / Resolution: 8.9 Å

PDB-5dfm:
Structure of Tetrahymena telomerase p19 fused to MBP
Method: X-RAY DIFFRACTION / Resolution: 2.301 Å

PDB-5dfn:
Structure of Tetrahymena Telomerase P45 C-terminal domain
Method: X-RAY DIFFRACTION / Resolution: 2.382 Å

PDB-5kmz:
Solution NMR structure of Tetrahymena telomerase RNA pseudoknot
Method: SOLUTION NMR

Chemicals

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-HOH:
WATER / Water

Source
  • tetrahymena thermophila (eukaryote)
  • escherichia coli (E. coli)
KeywordsNUCLEAR PROTEIN / Telomerase / P19 / CST complex / Ten1 / OB-fold / Oligonucleotide Binding Fold / P45 / Stn1 / Winged Helix / WH domain / WHtH / Winged Helix turn Helix / RNA / pseudoknot / triplex

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