PDB-1suv: Structure of Human Transferrin Receptor-Transferrin Complex

Entry
Summary
Database / ID	PORTEIN DATA BANK (PDB) / 1suv
Title	Structure of Human Transferrin Receptor-Transferrin Complex
Descriptor	Transferrin receptor protein 1 Serotransferrin
Keywords	METAL TRANSPORT, Protein Complex
Authors	Cheng, Y., Zak, O., Aisen, P., Harrison, S.C., Walz, T.
Date	Deposition: 2004-03-26, Release: 2004-04-13
PDBj Mine pages	Summary, Structural Details, Experimental Details, Functional Details
Other databases	RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST
Structure Visualization
Movies	Movie Page #1: Depositted structure unit, Made by Jmol
Structure viewers	Yorodumi, jV4, Jmol, Biological unit (Images, jV)
Related Structure Data
Related Entries	PDB-1a8e PDB-1cx8 PDB-1jnf
Similar strucutres (beta)	Diagram EMDB-1283 EMDB-2090 EMDB-1711 PDB-3zue List of similar structure data about Omokage system

Article
Citation - primary
Article	Cell, Vol. 116, Issue 4, Page 565-76, Year 2004
Title	Structure of the human transferrin receptor-transferrin complex.
Authors	Yifan Cheng, Olga Zak, Philip Aisen, Stephen C Harrison, Thomas Walz Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
Keywords	Animals, CHO Cells, Cricetinae, Cryoelectron Microscopy, Electrons, Humans, Image Processing, Computer-Assisted, Iron (chemistry, 7439-89-6), Microscopy, Electron, Models, Molecular, Protein Binding, Protein Conformation, Protein Structure, Tertiary, Receptors, Transferrin (chemistry), Time Factors, Transferrin (chemistry)
Links	PubMed: 14980223, PII: S0092867404001308

Components
ID 1 : TfR1, TR, TfR, Trfr, CD71 antigen, T9, p90
Image
Description	Transferrin receptor protein 1
Type	polymer
Formula weight	71623.695 Da
Number of molecules	2
Source	Method: Isolated from a genetically manipulated source Gene: human, Homo, TFRC, ID:9606, Homo sapiens Host: Chinese hamster, Cricetulus, ovary, ID:10029, Cricetulus griseus
Links	UniProt: P02786, Sequence view
ID 2 : Transferrin, Siderophilin, Beta-1-metal binding globulin, PRO1400
Image
Description	Serotransferrin, N-lobe
Type	polymer
Fragment	repeat 1
Formula weight	36408.684 Da
Number of molecules	2
Source	Method: Isolated from a genetically manipulated source Gene: human, Homo, TF, ID:9606, Homo sapiens Host: Chinese hamster, Cricetulus, kidney, ID:10029, Cricetulus griseus
Links	UniProt: P02787, Sequence view
ID 3 : Transferrin, Siderophilin, Beta-1-metal binding globulin
Image
Description	Serotransferrin, C-lobe
Type	polymer
Fragment	repeat 2
Formula weight	38300.738 Da
Number of molecules	2
Source	Method: Isolated from a genetically manipulated source Gene: human, Homo, TF, ID:9606, Homo sapiens Host: Chinese hamster, Cricetulus, kidney, ID:10029, Cricetulus griseus
Links	Sequence view
ID 4 : CARBONATE ION
Image
Description	CARBONATE ION
Type	non-polymer
Formula weight	60.009 Da
Number of molecules	4
Source	Method: Obtained synthetically
ID 5 : FE (III) ION
Image
Description	FE (III) ION
Type	non-polymer
Formula weight	55.847 Da
Number of molecules	4
Source	Method: Obtained synthetically

Sample
Assembly
Aggregation state	PARTICLE
Name	Human Transferrin Receptor - Transferrin Complex
Experiment
Reconstruction method	SINGLE PARTICLE
Specimen type	VITREOUS ICE (CRYO EM)
Sample preparation
pH	7.4

Processing
Single particle entity
Symmetry type	CYCLIC
3D reconstruction
Actual pixel size	2.74 A/pix
CTF correction method	CTF correction for each particle
Details	using program FREALIGN
Method	Fourier Space reconstruction
Nominal pixel size	2.8 A/pix
Resolution	7.5 A
3D fitting
Refinement Protocol	rigid body
Refinement Space	REAL
Target criteria	visual fit using program O followed by rigid body refinement using program MAVE
Refine hist
Total atoms	20572
Ligand atoms	20
Protein atoms	20552