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Structure of Human Transferrin Receptor-Transferrin Complex

by single particle reconstruction, at 7.5 A resolution

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#1: Depositted structure unit, Image by Jmol

Entry
Summary
Database / IDPORTEIN DATA BANK (PDB) / 1suv
TitleStructure of Human Transferrin Receptor-Transferrin Complex
DescriptorTransferrin receptor protein 1
Serotransferrin
KeywordsMETAL TRANSPORT, Protein Complex
AuthorsCheng, Y., Zak, O., Aisen, P., Harrison, S.C., Walz, T.
DateDeposition: 2004-03-26, Release: 2004-04-13
PDBj Mine pagesSummary, Structural Details, Experimental Details, Functional Details
Other databasesRCSB-PDB, PDBe, FSSP, SCOP
Structure Visualization
MoviesMovie Page

#1: Depositted structure unit, Image by Jmol

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Article
Citation - primary
ArticleCell, Vol. 116, Issue 4, Page 565-76, Year 2004
TitleStructure of the human transferrin receptor-transferrin complex.
AuthorsYifan Cheng, Olga Zak, Philip Aisen, Stephen C Harrison, Thomas Walz
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
KeywordsAnimals, CHO Cells, Cricetinae, Cryoelectron Microscopy, Electrons, Humans, Image Processing, Computer-Assisted, Iron (chemistry), Microscopy, Electron, Models, Molecular, Protein Binding, Protein Conformation, Protein Structure, Tertiary, Receptors, Transferrin (chemistry), Time Factors, Transferrin (chemistry)
LinksPubMed: 14980223, PII: S0092867404001308
Components
ID 1 : TfR1, TR, TfR, Trfr, CD71 antigen, T9, p90
Image
DescriptionTransferrin receptor protein 1
Typepolypeptide(L)
Formula weight71623.695 Da
Number of molecules2
ID1
SourceMethod: Isolated from a genetically manipulated source
Gene: human, Homo, TFRC, ID:9606, Homo sapiens
Host: Chinese hamster, Cricetulus, ovary, ID:10029, Cricetulus griseus
LinksUniProt: P02786, Sequence view
ID 2 : Transferrin, Siderophilin, Beta-1-metal binding globulin, PRO1400
Image
DescriptionSerotransferrin, N-lobe
Typepolypeptide(L)
Fragmentrepeat 1
Formula weight36408.684 Da
Number of molecules2
ID2
SourceMethod: Isolated from a genetically manipulated source
Gene: human, Homo, TF, ID:9606, Homo sapiens
Host: Chinese hamster, Cricetulus, kidney, ID:10029, Cricetulus griseus
LinksUniProt: P02787, Sequence view
ID 3 : Transferrin, Siderophilin, Beta-1-metal binding globulin
Image
DescriptionSerotransferrin, C-lobe
Typepolypeptide(L)
Fragmentrepeat 2
Formula weight38300.738 Da
Number of molecules2
ID3
SourceMethod: Isolated from a genetically manipulated source
Gene: human, Homo, TF, ID:9606, Homo sapiens
Host: Chinese hamster, Cricetulus, kidney, ID:10029, Cricetulus griseus
LinksSequence view
ID 4 : CARBONATE ION
Image
DescriptionCARBONATE ION
Typenon-polymer
Formula weight60.009 Da
Number of molecules4
ID4
SourceMethod: Obtained synthetically
ID 5 : FE (III) ION
Image
DescriptionFE (III) ION
Typenon-polymer
Formula weight55.847 Da
Number of molecules4
ID5
SourceMethod: Obtained synthetically
Sample
Assembly
Aggregation statePARTICLE
NameHuman Transferrin Receptor - Transferrin Complex
Experiment
Reconstruction methodSINGLE PARTICLE
Specimen typeVITREOUS ICE (CRYO EM)
Sample preparation
pH7.4
Experiment
MethodELECTRON MICROSCOPY
Electron Microscopy
Imaging
MicroscopeModel: FEI TECNAI F20
Date2001-10-15
Electron gun
Electron sourceFIELD EMISSION GUN
Accelerating voltage200 kV
Electron dose20 e/A**2
Illumination modeFLOOD BEAM
Lens
ModeBRIGHT FIELD
MagnificationCalibrated: 51160 X, Nominal: 50000 X
CsNominal: 2.0 mm
Nominal defocusMax: 5000 nm, Min: 2500 nm
Specimen holder
Tilt angleMin: 0 degrees, Max: 0 degrees
Temperature93 Kelvin
Detector
TypeKODAK SO163 FILM
Processing
Single particle entity
Symmetry typeCYCLIC
3D reconstruction
Actual pixel size2.74 A/pix
CTF correction methodCTF correction for each particle
Detailsusing program FREALIGN
MethodFourier Space reconstruction
Nominal pixel size2.8 A/pix
Resolution7.5 A
3D fitting
Refinement Protocolrigid body
Refinement SpaceREAL
Target criteriavisual fit using program O followed by rigid body refinement using program MAVE
3D fitting list
3D Fitting ID1
Refine hist
Cycle idLAST
Refine idELECTRON MICROSCOPY
Total atoms20572
Ligand atoms20
Protein atoms20552
Download
PDB format
Allpdb1suv.ent.gz
pdb1suv.ent (uncompressed file)
Header onlypdb1suv.ent.gz
mmCIF format
mmCIF1suv.cif.gz
XML format
All1suv.xml.gz
No-atom1suv-noatom.xml.gz
Ext-atom1suv-extatom.xml.gz
Movie files
movie #1
.mp4 (H.264/MPEG-4 AVC format), 2.9 MB
.webm (WebM/VP8 format), 3.8 MB