+Open data
-Basic information
Entry | Database: PDB / ID: 3cro | ||||||
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Title | THE PHAGE 434 CRO/OR1 COMPLEX AT 2.5 ANGSTROMS RESOLUTION | ||||||
Components |
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Keywords | TRANSCRIPTION/DNA / PROTEIN-DNA COMPLEX / DOUBLE HELIX / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Phage 434 (virus) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.5 Å | ||||||
Authors | Mondragon, A. / Harrison, S.C. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1991 Title: The phage 434 Cro/OR1 complex at 2.5 A resolution. Authors: Mondragon, A. / Harrison, S.C. #1: Journal: J.Mol.Biol. / Year: 1989 Title: Structure of Phage 434 Cro Protein at 2.35 Angstroms Resolution Authors: Mondragon, A. / Wolberger, C. / Harrison, S.C. #2: Journal: Science / Year: 1988 Title: Recognition of a DNA Operator by the Repressor of Phage 434. A View at High Resolution Authors: Aggarwal, A.K. / Rodgers, D.W. / Drottar, M. / Ptashne, M. / Harrison, S.C. #3: Journal: Nature / Year: 1987 Title: Structure of the Repressor-Operator Complex of Bacteriophage 434 Authors: Anderson, J.E. / Ptashne, M. / Harrison, S.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3cro.cif.gz | 61.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3cro.ent.gz | 42.7 KB | Display | PDB format |
PDBx/mmJSON format | 3cro.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3cro_validation.pdf.gz | 430.5 KB | Display | wwPDB validaton report |
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Full document | 3cro_full_validation.pdf.gz | 449.4 KB | Display | |
Data in XML | 3cro_validation.xml.gz | 10.9 KB | Display | |
Data in CIF | 3cro_validation.cif.gz | 14.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cr/3cro ftp://data.pdbj.org/pub/pdb/validation_reports/cr/3cro | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | THE TWO PROTEIN DOMAINS HAVE BEEN ASSIGNED CHAIN INDICATORS *L* AND *R*. THE TWO DNA CHAINS HAVE BEEN ASSIGNED CHAIN INDICATORS *A* AND *B*. THE UNIT CELL CONTAINS TWO COMPLEXES EACH OF WHICH CONSISTS OF A PROTEIN DIMER AND TWO DIFFERENT STRANDS OF DNA. |
-Components
#1: DNA chain | Mass: 6106.990 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||||
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#2: DNA chain | Mass: 6156.027 Da / Num. of mol.: 1 / Source method: obtained synthetically | ||||
#3: Protein | Mass: 8076.619 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Phage 434 (virus) / Genus: Lambda-like viruses / Species: Enterobacteria phage lambda / References: UniProt: P03036 #4: Water | ChemComp-HOH / | Sequence details | THE DNA CHAINS ARE ALIGNED AS FOLLOWS *B* 5(PRIME) T-A-T-A-C-A-A-G-A-A-A-G-T-T-T-G-T-A-C-T *A* ...THE DNA CHAINS ARE ALIGNED AS FOLLOWS *B* 5(PRIME) T-A-T-A-C-A-A-G-A-A-A-G-T-T-T-G-T-A-C-T *A* 3(PRIME) T-A-T-G-T-T-C-T-T-T-C-A-A-A-C-A-T-G-A-A THE DNA CHAINS ARE NUMBERED SEQUENTIAL | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.95 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / pH: 6.2 / Details: pH 6.20, temperature 277.00K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 4 ℃ / Method: unknown / pH: 6.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ROTATING ANODE / Type: ELLIOTT GX-13 |
Detector | Type: XENTRONICS / Detector: AREA DETECTOR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Highest resolution: 2.5 Å / Num. obs: 7232 |
Reflection | *PLUS Highest resolution: 2.5 Å / Rmerge(I) obs: 0.101 |
-Processing
Software | Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
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Refinement | Resolution: 2.5→10 Å Details: DENSITY FOR THE LAST SIX CARBOXY TERMINAL RESIDUES (64 - 70) WAS NOT OBSERVED IN THE MAP AT ANY STAGE OF THE REFINEMENT AND THESE RESIDUES ARE PROBABLY DISORDERED.
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Refinement step | Cycle: LAST / Resolution: 2.5→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 10 Å / Rfactor obs: 0.22 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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