+Open data
-Basic information
Entry | Database: PDB / ID: 2fyz | ||||||
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Title | Structural of Mumps virus fusion protein core | ||||||
Components | (Fusion glycoprotein F0) x 2 | ||||||
Keywords | PROTEIN BINDING / Mumps virus fusion protein core | ||||||
Function / homology | Function and homology information membrane => GO:0016020 / fusion of virus membrane with host plasma membrane / viral envelope / host cell plasma membrane / virion membrane Similarity search - Function | ||||||
Biological species | Mumps virus | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Lou, Z. / Xu, Y. / Liu, Y. / Rao, Z. | ||||||
Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2006 Title: Structural characterization of mumps virus fusion protein core Authors: Liu, Y. / Xu, Y. / Lou, Z. / Zhu, J. / Hu, X. / Gao, G.F. / Qiu, B. / Rao, Z. / Tien, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fyz.cif.gz | 70 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fyz.ent.gz | 52.5 KB | Display | PDB format |
PDBx/mmJSON format | 2fyz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2fyz_validation.pdf.gz | 468.4 KB | Display | wwPDB validaton report |
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Full document | 2fyz_full_validation.pdf.gz | 478 KB | Display | |
Data in XML | 2fyz_validation.xml.gz | 16.3 KB | Display | |
Data in CIF | 2fyz_validation.cif.gz | 22.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fy/2fyz ftp://data.pdbj.org/pub/pdb/validation_reports/fy/2fyz | HTTPS FTP |
-Related structure data
Related structure data | 1svfS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | There are 3 molecules in one ASU. They form the biological unit. |
-Components
#1: Protein | Mass: 6694.612 Da / Num. of mol.: 3 / Fragment: residues 119-181 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mumps virus / Genus: Rubulavirus / Plasmid: pGEX-6p-1 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P11236 #2: Protein/peptide | Mass: 5080.690 Da / Num. of mol.: 3 / Fragment: residues 438-485 / Mutation: A463T, N478I Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mumps virus / Genus: Rubulavirus / Plasmid: pGEX-6p-1 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P11236 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.75 Å3/Da / Density % sol: 55.35 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 15% PEG 800, 0.5M Lithium Sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 Å |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE |
Radiation | Monochromator: osmic mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 19670 / Num. obs: 17183 |
Reflection shell | Resolution: 2.2→2.3 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1SVF Resolution: 2.2→50 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
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Refine LS restraints |
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