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Yorodumi- PDB-1yx0: Solution Structure of Bacillus subtilis Protein ysnE: The Northea... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1yx0 | ||||||
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Title | Solution Structure of Bacillus subtilis Protein ysnE: The Northeast Structural Genomics Consortium Target SR220 | ||||||
Components | hypothetical protein ysnE | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / NESG / GFT NMR / Structral Genomics / SR220 / PSI / Protein Structure Initiative / Northeast Structural Genomics Consortium | ||||||
Function / homology | Function and homology information N-acetyltransferase activity / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups Similarity search - Function | ||||||
Biological species | Bacillus subtilis subsp. subtilis str. 168 (bacteria) | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing torsion angle dynamics | ||||||
Authors | Liu, G. / Ma, L. / Shen, Y. / Xiao, R. / Acton, T. / Montelione, G.T. / Szyperski, T. / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: To be Published Title: Solution Structure of Bacillus subtilis Protein ysnE Authors: Liu, G. / Ma, L. / Shen, Y. / Xiao, R. / Acton, T. / Montelione, G.T. / Szyperski, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1yx0.cif.gz | 923.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1yx0.ent.gz | 772.8 KB | Display | PDB format |
PDBx/mmJSON format | 1yx0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1yx0_validation.pdf.gz | 344.1 KB | Display | wwPDB validaton report |
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Full document | 1yx0_full_validation.pdf.gz | 566.5 KB | Display | |
Data in XML | 1yx0_validation.xml.gz | 70.4 KB | Display | |
Data in CIF | 1yx0_validation.cif.gz | 87.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yx/1yx0 ftp://data.pdbj.org/pub/pdb/validation_reports/yx/1yx0 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 18148.590 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis subsp. subtilis str. 168 (bacteria) Species: Bacillus subtilis / Strain: subsp. subtilis str. 168 / Gene: ysnE / Production host: Escherichia coli (E. coli) / References: GenBank: 16079885, UniProt: P94562*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using GFT NMR techniques. |
-Sample preparation
Details | Contents: NMR Buffer 6.5 -- 5% D2O, 0.02% NaN3, 10mM DTT, 5mM CaCl2, 100mM NaCl, 20mM MES, pH 6.5 Solvent system: 90% H2O/10% D2O |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: distance geometry simulated annealing torsion angle dynamics Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |