[English] 日本語
Yorodumi- PDB-1tib: CONFORMATIONAL LABILITY OF LIPASES OBSERVED IN THE ABSENCE OF AN ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1tib | ||||||
---|---|---|---|---|---|---|---|
Title | CONFORMATIONAL LABILITY OF LIPASES OBSERVED IN THE ABSENCE OF AN OIL-WATER INTERFACE: CRYSTALLOGRAPHIC STUDIES OF ENZYMES FROM THE FUNGI HUMICOLA LANUGINOSA AND RHIZOPUS DELEMAR | ||||||
Components | LIPASE | ||||||
Keywords | HYDROLASE(CARBOXYLIC ESTERASE) | ||||||
Function / homology | Function and homology information triacylglycerol lipase / triacylglycerol lipase activity / lipid catabolic process Similarity search - Function | ||||||
Biological species | Thermomyces lanuginosus (fungus) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.84 Å | ||||||
Authors | Derewenda, U. / Swenson, L. / Wei, Y. / Derewenda, Z.S. | ||||||
Citation | Journal: J.Lipid Res. / Year: 1994 Title: Conformational lability of lipases observed in the absence of an oil-water interface: crystallographic studies of enzymes from the fungi Humicola lanuginosa and Rhizopus delemar. Authors: Derewenda, U. / Swenson, L. / Wei, Y. / Green, R. / Kobos, P.M. / Joerger, R. / Haas, M.J. / Derewenda, Z.S. #1: Journal: Nat.Struct.Biol. / Year: 1994 Title: An Unusual Buried Polar Cluster in a Family of Fungal Lipases Authors: Derewenda, U. / Swenson, L. / Green, R. / Wei, Y. / Dodson, G.G. / Yamaguchi, S. / Haas, M.J. / Derewenda, Z.S. #2: Journal: Protein Eng. / Year: 1994 Title: Current Progress in Crystallographic Studies of New Lipases from Filamentous Fungi Authors: Derewenda, U. / Swenson, L. / Green, R. / Wei, Y. / Yamaguchi, S. / Joerger, R. / Haas, M.J. / Derewenda, Z.S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1tib.cif.gz | 73 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1tib.ent.gz | 53.8 KB | Display | PDB format |
PDBx/mmJSON format | 1tib.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1tib_validation.pdf.gz | 367.4 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1tib_full_validation.pdf.gz | 383.6 KB | Display | |
Data in XML | 1tib_validation.xml.gz | 8.9 KB | Display | |
Data in CIF | 1tib_validation.cif.gz | 14.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ti/1tib ftp://data.pdbj.org/pub/pdb/validation_reports/ti/1tib | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Atom site foot note | 1: CIS PROLINE - PRO 207 / 2: CIS PROLINE - PRO 218 |
-Components
#1: Protein | Mass: 29342.484 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermomyces lanuginosus (fungus) / References: UniProt: O59952, triacylglycerol lipase |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.09 Å3/Da / Density % sol: 41.13 % | ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Reflection | *PLUS Highest resolution: 1.85 Å / % possible obs: 100 % / Rmerge(I) obs: 0.0472 |
---|
-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.84→7.5 Å / σ(F): 0 /
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.84→7.5 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.18 / Rfactor obs: 0.167 / Highest resolution: 1.85 Å / Num. reflection obs: 18756 / Rfactor Rwork: 0.167 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
|